BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0035.p (317 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate A... 466 e-133 gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate A... 324 7e-90 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 97 2e-21 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 88 9e-19 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 82 5e-17 >gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 466 bits (1200), Expect = e-133, Method: Compositional matrix adjust. Identities = 233/288 (80%), Positives = 259/288 (89%) Query: 30 MHLSPPIRDEYEIALTPLDDVPLERTLSLPQRIWQQSWVRKTVILLVLALIWEIVARVQD 89 M +PPIRDEYEI LTPL + L+R SLPQR+WQQ WVRK ILL+LAL+WE VAR Q+ Sbjct: 1 MAQTPPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQN 60 Query: 90 NDLLLPGFVQTAHAFVDGVATGELIGKVWISLKVLVKGYLIGIALAFGLTTLAVSTQLGR 149 NDLLLP F+QTA AF+DG+ +GEL GK W+SL+VL+K Y++GI AF LTTLAVSTQ GR Sbjct: 61 NDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGR 120 Query: 150 DLLSTLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTL 209 DLL TLTSMFNPLPAIA+LP+ALLWFGLG+ SLIFVLVHSVLWALA+NTYAGFLGVSDTL Sbjct: 121 DLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTL 180 Query: 210 RMAGRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGW 269 RMAGRNYGLRGL FVL +LVPAALPSILAGLRIGWAFAWRTLIAAELVFGA+SGKGGLGW Sbjct: 181 RMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGW 240 Query: 270 YIFQNRNELYTDKVFAGLAMVILIGLVVENFGFNTIERLTVKRWGMQR 317 YIFQNRNELYTDKVFAGLAMVILIGL+VEN FNT+ER+TVK+WGMQR Sbjct: 241 YIFQNRNELYTDKVFAGLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 >gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 289 Score = 324 bits (830), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 172/289 (59%), Positives = 217/289 (75%), Gaps = 1/289 (0%) Query: 30 MHLSPP-IRDEYEIALTPLDDVPLERTLSLPQRIWQQSWVRKTVILLVLALIWEIVARVQ 88 MH + P +R E + P ++R LS + +++ W+RKTVILLVLA IWE+ R Sbjct: 1 MHTNLPFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWL 60 Query: 89 DNDLLLPGFVQTAHAFVDGVATGELIGKVWISLKVLVKGYLIGIALAFGLTTLAVSTQLG 148 DN LL P F +T AF+ G+ +G L+ + +S++ L+ GY +GI LA LTT+A+S+++G Sbjct: 61 DNALLFPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIG 120 Query: 149 RDLLSTLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDT 208 DLL TLTSMFNPLPAIA+LPLAL+WFGLG S+IFVLVHSVLWA+ALNT+ GF VS+T Sbjct: 121 NDLLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNT 180 Query: 209 LRMAGRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLG 268 LRM G+NYGLR V +L+PAA PSIL GL++GWAFAWRTLIAAELVFG SSG GGLG Sbjct: 181 LRMVGQNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLG 240 Query: 269 WYIFQNRNELYTDKVFAGLAMVILIGLVVENFGFNTIERLTVKRWGMQR 317 WYIF+NRN+L T VF+GL VI+IGLVVEN F IE+ T++RWGMQ Sbjct: 241 WYIFENRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 67/189 (35%), Positives = 105/189 (55%), Gaps = 4/189 (2%) Query: 110 TGELIGKVWISLKVLVKGYLIGIALAFGLTTLAVSTQLGRDLLSTLTSMFNPLPAIAMLP 169 +GEL+ SL +V G+LIG LA L L S+Q L++ + P+P IA +P Sbjct: 70 SGELVMDAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIP 129 Query: 170 LALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTLRMAGRNYGLRGLRFVLFMLV 229 LA+LWFGLG +F++ + + +NT AG V A RN G L +++ Sbjct: 130 LAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVML 189 Query: 230 PAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGWYIFQNRNELYTDKVFAGLAM 289 PAA+P IL+G+RIG A+ +I AE++ + GLG+ I + R ++DK+ AG+ Sbjct: 190 PAAVPYILSGVRIGIGTAFIVVIVAEMI----AVSNGLGFRIMEAREYFWSDKIIAGMIT 245 Query: 290 VILIGLVVE 298 + L+GL ++ Sbjct: 246 IGLLGLAID 254 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 88.2 bits (217), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 68/220 (30%), Positives = 109/220 (49%), Gaps = 5/220 (2%) Query: 81 WEIVARVQ-DNDLLLPGFVQTAHAFVDGVATGELIGKVWISLKVLVKGYLIGIALAFGLT 139 WE+ A+ + +LP + A + A+GEL + +S G+ +G L L Sbjct: 47 WEVAAQAGWLSSRILPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILG 106 Query: 140 TLAVSTQLGRDLLSTLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTY 199 L+ S + LL T M +PA+A++PL +LWFG+ E + +F++ V + + LNT+ Sbjct: 107 LLSGSLRSAELLLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTF 166 Query: 200 AGFLGVSDTLRMAGRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFG 259 G V L ++YGL G +++P ALP+IL GLR W LI AE + Sbjct: 167 HGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETI-- 224 Query: 260 ASSGKGGLGWYIFQNRNELYTDKVFAGLAMVILIGLVVEN 299 S + G+G+ R L TD V G+ + L+G + + Sbjct: 225 --SAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADT 262 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 82.4 bits (202), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 58/189 (30%), Positives = 89/189 (47%), Gaps = 4/189 (2%) Query: 93 LLPGFVQTAHAFVDGVATGELIGKVWISLKVLVKGYLIGIALAFGLTTLAVSTQLGRDLL 152 LLP + AH GEL+ + SL + G++IG ALA L L ++ LL Sbjct: 52 LLPPPSELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALL 111 Query: 153 STLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTLRMA 212 +P++A +PL LLW G+ E + ++ + + LN AG + L Sbjct: 112 EPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEV 171 Query: 213 GRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGWYIF 272 G GL + +L+PA+LP + AGLR G + AW L+AAEL+ + G+G+ + Sbjct: 172 GTTSGLNTQALIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELI----AATKGVGYLLS 227 Query: 273 QNRNELYTD 281 R D Sbjct: 228 DGRESSRPD 236 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 995,471 Number of extensions: 41847 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 15 Length of query: 317 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 232 Effective length of database: 1,217,395 Effective search space: 282435640 Effective search space used: 282435640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)