BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0025.p (175 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309413|ref|YP_003773505.1| methyl transferase [Herbaspiril... 270 3e-74 >gi|300309413|ref|YP_003773505.1| methyl transferase [Herbaspirillum seropedicae SmR1] Length = 232 Score = 270 bits (691), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 128/164 (78%), Positives = 143/164 (87%), Gaps = 2/164 (1%) Query: 4 FDTGCGVGLSTLNLATQFPDHFVIGIDQSEDRLGRNTQWDGPPPGNLCFIRADLVDYWRL 63 DTGCGVGLSTL+LA+Q PDHFVIGIDQS DRL R+T WDGP P NLCF+RADLVDYWRL Sbjct: 65 LDTGCGVGLSTLHLASQHPDHFVIGIDQSADRLARHTHWDGPEPANLCFVRADLVDYWRL 124 Query: 64 LLEAKVFPARQYLLYPNPWPKIGHLARRWRWHGHAVFPSIIALGGVIECRSNWRIYVEEF 123 L+A V PAR YLLYPNPWPKIGHLARRW+ GHAVFP+I+ALGG IECRSNWRIYVEEF Sbjct: 125 ALQAGVRPARHYLLYPNPWPKIGHLARRWQ--GHAVFPAIVALGGQIECRSNWRIYVEEF 182 Query: 124 AAALTQLSGRPTACEAYATDTPITPFERKYLASGHALWRCRSEL 167 AAAL+QLSG+ EA+ + PITPFERKYLASGHALWRC+++L Sbjct: 183 AAALSQLSGQRIQAEAWRPERPITPFERKYLASGHALWRCQADL 226 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.482 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 656,080 Number of extensions: 28361 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 61 Number of HSP's successfully gapped: 1 Length of query: 175 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 96 Effective length of database: 1,245,805 Effective search space: 119597280 Effective search space used: 119597280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)