BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0011.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 573 e-164 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 336 2e-93 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 176 2e-45 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 122 3e-29 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 122 7e-29 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 573 bits (1476), Expect = e-164, Method: Compositional matrix adjust. Identities = 278/320 (86%), Positives = 304/320 (95%) Query: 13 LEGAQLIGAGLLLAAANFIAVLDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITV 72 L G QL+GAG+LLAAANFIAVLDTTIANVSVSTI+GALGAS+SQGTYVITSYAVAEAITV Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 73 PLTGWLANRFGTVRVFITSMILFGVFSALCGLANSLGMLIAFRVLQGLAGGPLMPLSQTL 132 PLTGWLANRFGTVRVFI SM++FG+FSALCG+ANSLGMLIAFRVLQGLAGGPLMPLSQTL Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 133 LLRIFPKEKAPAAVGLWAMTTLIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGW 192 LLRIFPKEKAPAAVGLWAMTTL+APIAGPILGGVLCDQYSWPYIF+INVPVALICGY+GW Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 193 KMLKRYESPLLKLPIDKIGLILMIVWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFI 252 +MLKRYE+ + KLPID++GL+LM+VWVAALQLMLDEGKEKDWFASTEIV+LA+ A FI Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFI 265 Query: 253 AFMIWELTERNPIVDLRVFRHRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHS 312 AF+IWELTERNPIVDLRVFRHRG+TASVTTICLAFGAFFG+ VLTPLWLQGYMGYTAT S Sbjct: 266 AFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 325 Query: 313 GETTALTGILAVLTAPKCRR 332 G +ALTGILAV+TAP + Sbjct: 326 GCISALTGILAVMTAPMVAK 345 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 336 bits (861), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 169/327 (51%), Positives = 227/327 (69%), Gaps = 3/327 (0%) Query: 12 PLEGAQLIGAGLLLAAANFIAVLDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAIT 71 PL GA L+ L ++ A F+ VLD++IANV++ TISG LG S +GT+VITS+A A AI+ Sbjct: 16 PLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAIS 75 Query: 72 VPLTGWLANRFGTVRVFITSMILFGVFSALCGLANSLGMLIAFRVLQGLAGGPLMPLSQT 131 +PLTGWL R G VR+F+TS++LF + S LCGLA +L +L+A RVLQGL GP++PLSQ+ Sbjct: 76 IPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQS 135 Query: 132 LLLRIFPKEKAPAAVGLWAMTTLIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMG 191 LLL +PK K+ A+ LW MT ++AP+AGP LGG + D Y+WP+IF+IN+PV LI + Sbjct: 136 LLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVT 195 Query: 192 WKMLKRYESPLLKLPIDKIGLILMIVWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGF 251 + + E+P KLPIDKIGL L++ WVAA Q+MLD+GK+ DWFAS IV L +IA F Sbjct: 196 LAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAF 255 Query: 252 IAFMIWELTERNPIVDLRVFRHRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATH 311 F++WEL +P+VDLR+F R + I + +G FFG V+ P WLQ Y+GY + Sbjct: 256 AYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSID 315 Query: 312 SGETTALTGILAVLTAPKCRRPDEFLP 338 SG +TA GI AV+ P R FLP Sbjct: 316 SGLSTAPIGIFAVILMPLIGR---FLP 339 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 176 bits (447), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 104/303 (34%), Positives = 170/303 (56%), Gaps = 7/303 (2%) Query: 30 FIAVLDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI 89 +A LD +I N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G + + Sbjct: 27 LMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVLLL 86 Query: 90 TSMILFGVFSALCGLANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLW 149 T+ LF +FS LCGL+ +L M+I RV QG GG L+P + T++ P+ + P ++ Sbjct: 87 TAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMF 146 Query: 150 AMTTLIAPIAGPILGGVLCDQYSWPYIFFINVPV--ALICGYMGWKMLKRYESPLLK--L 205 T ++ P+ GP++GG L + SW Y FFIN+PV ALI + + YE P + Sbjct: 147 GATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLL---VAIPYERPKFEQLA 203 Query: 206 PIDKIGLILMIVWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFMIWELTERNPI 265 D G+ + + + L ++L+EG+ + WF+S +I LA + GFI I ++ R+P+ Sbjct: 204 EADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPV 263 Query: 266 VDLRVFRHRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHSGETTALTGILAVL 325 + LR+ R R + + V + A +G + P +L G GY + SG L+G+ +L Sbjct: 264 IQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMIL 323 Query: 326 TAP 328 P Sbjct: 324 MMP 326 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 122 bits (307), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 85/291 (29%), Positives = 147/291 (50%), Gaps = 6/291 (2%) Query: 24 LLAAANFIAVLDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFG 83 ++AA F+ LDTTI ++ ++ + G ++ + +T+Y + A+ +P++GW+A+RFG Sbjct: 35 IVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFG 94 Query: 84 TVRVFITSMILFGVFSALCGLANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAP 143 + VF ++ +F + S LCG + ++ A RVLQGL G ++P+ + +++R K++ Sbjct: 95 SRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLM 154 Query: 144 AAVGLWAMTTLIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGWKMLKRYESPLL 203 A+ ++AP+ GP +GG + SW +IF +NVP L + M+ R E + Sbjct: 155 KAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGL-AAFTAVMMVVRNEHGVS 213 Query: 204 KLPIDKIGLILMIVWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFMIWELTER- 262 K P+D +G +L AAL +L + S V L +A + + W +R Sbjct: 214 KRPLDLVGFLLS---GAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRI 270 Query: 263 -NPIVDLRVFRHRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHS 312 +P++D R Y+ +V T L L PL Q G TA S Sbjct: 271 EHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQS 321 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 122 bits (305), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 79/289 (27%), Positives = 151/289 (52%), Gaps = 14/289 (4%) Query: 34 LDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFITSMI 93 +D+T+ + S+ ++ + +TSY V+ A+ +P++GW+A+RFG++ +F +++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 94 LFGVFSALCGLANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTT 153 +F + S LCG++++L +A R LQG+ G ++P+ + ++LR K A+ + Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 154 LIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGWKMLKRYESPLLKLPIDKIGLI 213 L P+ GP LGG + + W +IFFIN+P++++ Y+ + + ++ + P+D G + Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVA-PLDLKGFL 179 Query: 214 LMIVWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFMIWELTERN-PIVDLRVFR 272 L + + L + L S E+ + ++A F+A +W + P++DL + + Sbjct: 180 LSALGCSLLMMGLATAGRH--LVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLK 237 Query: 273 HRGYTASVTTICLAFGAFFGATV-----LTPLWLQGYMGYTATHSGETT 316 A V G+F+ + L PL LQ GYT HSG T Sbjct: 238 IPTLRAGVFG-----GSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLT 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,206,799 Number of extensions: 50670 Number of successful extensions: 299 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 21 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)