BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0099.0005.p
         (226 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313899|ref|YP_003777991.1| RND family efflux transporter M...   185   2e-48

>gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP
           subunit [Herbaspirillum seropedicae SmR1]
          Length = 406

 Score =  185 bits (470), Expect = 2e-48,   Method: Compositional matrix adjust.
 Identities = 86/123 (69%), Positives = 109/123 (88%)

Query: 81  SDASXQKFDGKVARTAASIDTATRTMQVEVSLPNPDGALMPGAFVQVALPLKPSGALVVS 140
           S+   +KF+GKVARTA +ID ATR+MQVEV+L N D AL+PGAFVQVALP+KPSGAL+V+
Sbjct: 267 SELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVALPIKPSGALLVA 326

Query: 141 TDVLLFRREGTMVAAVDAGNKIRLKRVQIGRNFGQTVEVLDGITGDERLILNPSDSVAEG 200
            D L+FRR+GT+VAAVD  N++RLK++++GRNFGQTVEVLDG+ GDE LILNPSD++AEG
Sbjct: 327 ADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQGDEHLILNPSDALAEG 386

Query: 201 DEV 203
           D+V
Sbjct: 387 DQV 389



 Score = 66.2 bits (160), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 50/81 (61%), Positives = 67/81 (82%), Gaps = 3/81 (3%)

Query: 1  MSQERHSQLGIHPLDPGEAGELLKRRQIVRRTRLIVIIVLVLLAAGAARTVISRVFNARE 60
          MS +RHS+LGIHPL  GE  +   R+Q++RR R+++++VL+LLAAGAARTVISRV NARE
Sbjct: 1  MSHDRHSELGIHPLSEGEPQQ---RQQMMRRARVVLVVVLLLLAAGAARTVISRVANARE 57

Query: 61 LEAGTAERARQYVKTAIAKSS 81
          L+AGTAERA+ +V+ A  +SS
Sbjct: 58 LQAGTAERAKMFVRVAPVRSS 78


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.131    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 548,652
Number of extensions: 18158
Number of successful extensions: 83
Number of sequences better than 1.0e-08: 5
Number of HSP's gapped: 85
Number of HSP's successfully gapped: 6
Length of query: 226
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 144
Effective length of database: 1,231,600
Effective search space: 177350400
Effective search space used: 177350400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 129 (54.3 bits)