BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0004.p (292 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 308 4e-85 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 70 2e-13 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 58 9e-10 gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 55 1e-08 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 308 bits (788), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 154/190 (81%), Positives = 162/190 (85%), Gaps = 6/190 (3%) Query: 1 MGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASY 60 +GKPLHDAIIVGAGEIGVPAFV+T+CICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASY Sbjct: 416 LGKPLHDAIIVGAGEIGVPAFVATLCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASY 475 Query: 61 ILSRTLVPTLVMLLMGGVHGAQGDTGGGKPSLLQRLYRGFDSRFEHVRRAYTLVLSSLLS 120 +LSRTLVPTLVMLLMG + G K SLLQRLYR FD+RFE VR YTLVLSSLL+ Sbjct: 476 LLSRTLVPTLVMLLMG----SHGQVDSAKQSLLQRLYRAFDNRFERVRHGYTLVLSSLLA 531 Query: 121 HRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIEETARGGRLPRH 180 HRRRFALAFLGFC+LSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIEETAR Sbjct: 532 HRRRFALAFLGFCLLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIEETARAADEVER 591 Query: 181 --RVLFPAGR 188 R L PA Sbjct: 592 VIRTLIPASE 601 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 70.5 bits (171), Expect = 2e-13, Method: Composition-based stats. Identities = 43/150 (28%), Positives = 71/150 (47%), Gaps = 10/150 (6%) Query: 7 DAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASYILSRTL 66 +A + GAGEIG +V + VF+P+ + G+ LF A V +A S I+S TL Sbjct: 434 EAALKGAGEIGFTIISISVSLVAVFIPLLLMGGIVGRLFREFAVTVTLTIAVSVIISLTL 493 Query: 67 VPTLVMLLMGGVHGAQGDTGGGKPSLLQRLYRGFDSRFEHVRRAYTLVLSSLLSHRRRFA 126 P L + HG + +LY+ F+ F+ + Y L ++ H+ Sbjct: 494 TPMLCSRFLKNEHGQRHG----------KLYQWFERCFDAMLAGYKRGLDVVMKHQFITL 543 Query: 127 LAFLGFCVLSCLLYPFLGRDFFPNVDGGQI 156 ++F+ L+ +L+ F+ + FFP D G I Sbjct: 544 MSFIATLALTAVLFIFIPKGFFPQQDNGVI 573 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 58.2 bits (139), Expect = 9e-10, Method: Composition-based stats. Identities = 41/146 (28%), Positives = 64/146 (43%), Gaps = 14/146 (9%) Query: 30 VFVPMFFLSGVARFLFVPLAEAVVFAMAASYILSRTLVPTLVMLLM---GGVHGAQGDTG 86 VF+PM F G ++ + +V AMA S +++ TL P L L+ G H QG Sbjct: 454 VFIPMAFFGGSTGVIYRQFSITIVSAMALSVMVALTLTPALCATLLKPHAGGHVQQG--- 510 Query: 87 GGKPSLLQRLYRGFDSRFEHVRRAYTLVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRD 146 R + F+ F+ Y ++ LL +R L + V +L+ L Sbjct: 511 --------RFFTWFNRSFDRSATRYQGGVAGLLKRGKRGLLMYALIAVAMAVLFMRLPTS 562 Query: 147 FFPNVDGGQIRLHMRMPTGTRIEETA 172 F P D G + +++PTG E+TA Sbjct: 563 FLPEEDQGVLMAQIQLPTGATAEQTA 588 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 54.7 bits (130), Expect = 1e-08, Method: Composition-based stats. Identities = 44/165 (26%), Positives = 80/165 (48%), Gaps = 21/165 (12%) Query: 9 IIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASYILSRTLVP 68 + A E P + I IV++PMF L+GV +F P+A VV A+ + ILS T +P Sbjct: 441 VFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGAMILSVTFIP 500 Query: 69 TLVMLLMGGVHGAQGDTGGGKPSLLQRLYRGFDSRFEHVRRAYTLVLSSLLSHRRRFALA 128 + L + G+ K ++L +R Y +L +++ + + L Sbjct: 501 AAIALFI-------GEKVAEKENILMLW----------AKRLYEPMLKTIMEN-KPVVLT 542 Query: 129 FLGFCVLSC-LLYPFLGRDFFPNVDGGQIRLH-MRMPTGTRIEET 171 F G +L C ++ LG +F P+++ G + + +R+P GT + ++ Sbjct: 543 FAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIP-GTSLSQS 586 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 728,888 Number of extensions: 26758 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 5 Length of query: 292 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 208 Effective length of database: 1,222,130 Effective search space: 254203040 Effective search space used: 254203040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)