BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0099.0003.p
         (271 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr...   458   e-130
gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr...    65   5e-12
gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr...    55   4e-09

>gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump
           protein [Herbaspirillum seropedicae SmR1]
          Length = 1063

 Score =  458 bits (1178), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 221/248 (89%), Positives = 240/248 (96%)

Query: 1   MSMVELALRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMG 60
           M+MV LALRRPYTFIVMA+LIVL+TPL L  MATDIFPEINIPV+SIIWNYNGLSAQEMG
Sbjct: 1   MNMVALALRRPYTFIVMALLIVLATPLTLKNMATDIFPEINIPVVSIIWNYNGLSAQEMG 60

Query: 61  QRIAAQNERGLTTVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQL 120
           QRIAAQNERGLTTVVSDIEHIESQSLAGV+VIKVFFQP ANIQ AIAQ VASVQ+Q+RQL
Sbjct: 61  QRIAAQNERGLTTVVSDIEHIESQSLAGVSVIKVFFQPKANIQTAIAQVVASVQTQVRQL 120

Query: 121 PPGITPPLVIKYSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYGG 180
           PPGITPPLVIKYSASSIPVMQLALSSPTLPEQ++FDAA+ TLRPQL+TIPGVAVP+PYGG
Sbjct: 121 PPGITPPLVIKYSASSIPVMQLALSSPTLPEQTVFDAAVQTLRPQLVTIPGVAVPYPYGG 180

Query: 181 KTRLISVDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFGATEYTVKMNGSPEAVAG 240
           K+RLISVDLDTQALQARGL+P+DVVNA+NTQNLILPSGTAKFGATEY+VK+NGSPEAVAG
Sbjct: 181 KSRLISVDLDTQALQARGLAPIDVVNAINTQNLILPSGTAKFGATEYSVKINGSPEAVAG 240

Query: 241 LNDLPVRT 248
           LN+LPVRT
Sbjct: 241 LNELPVRT 248


>gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump
           protein [Herbaspirillum seropedicae SmR1]
          Length = 1051

 Score = 65.1 bits (157), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 65/259 (25%), Positives = 109/259 (42%), Gaps = 10/259 (3%)

Query: 1   MSMVELALRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMG 60
           MS+    ++RP    ++A+ I+L    V   +     P+++ P I +     G S + M 
Sbjct: 1   MSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60

Query: 61  QRIAAQNERGLTTVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQL 120
             +A   ER  + +++ +  + SQS  G   I + F    NI  A     A++ +   QL
Sbjct: 61  SNVAQPLERQFS-LIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQL 119

Query: 121 PPGI-TPPLVIKYSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYG 179
           P  +  PP   K + +  P+M +++ S  LP   + D A N L  Q+  I GV +    G
Sbjct: 120 PANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNG 179

Query: 180 GKTRLISVDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFGATEYTVKMNGSPEAVA 239
            +   + + +D   L   GLS  D+   + T  +  P GT       +TV  N       
Sbjct: 180 QQKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAE 239

Query: 240 GLNDL--------PVRTKK 250
             ND+        P+R K 
Sbjct: 240 QWNDMVLAWRNGAPIRVKD 258


>gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump
           protein [Herbaspirillum seropedicae SmR1]
          Length = 1078

 Score = 55.5 bits (132), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 57/252 (22%), Positives = 109/252 (43%), Gaps = 10/252 (3%)

Query: 8   LRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMGQRIAAQN 67
           ++RP    ++ + I L+  +    +     P+++ P ISI  +  G S + M   +A   
Sbjct: 8   IQRPIATTLLTIGIALAGIVAFRLLPVSPLPQVDYPTISISASMPGASPETMAATVATPL 67

Query: 68  ERGLTTVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQLPPGI-TP 126
           ER +   ++ +  + S S      I + F  + +I  A     A++ +    LP G+ + 
Sbjct: 68  ERAMG-AIAGVTEMTSTSSQNSTRITLQFDLSRDIDGAARDVQAALNAARNLLPTGMPSN 126

Query: 127 PLVIKYSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYGGKTRLIS 186
           P   K + +  P++ LA++S T+ +  ++DAA + +  +L  + GV      G     + 
Sbjct: 127 PTYRKVNPADAPILILAMTSDTMTQGQMYDAADSIVAQKLSQVRGVGQVSVGGSSQPAVR 186

Query: 187 VDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFGATEYTVKMNG--------SPEAV 238
           ++L+  AL   G+   DV  AV   N   P G  +     + +  N         +P  V
Sbjct: 187 IELNPTALNKYGIGTADVRTAVAATNANRPKGLLEDQDRNWQIYANDQAKTAAEYAPLIV 246

Query: 239 AGLNDLPVRTKK 250
           +  N  PVR + 
Sbjct: 247 SYRNGTPVRVQD 258


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.133    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 744,255
Number of extensions: 29313
Number of successful extensions: 141
Number of sequences better than 1.0e-08: 9
Number of HSP's gapped: 135
Number of HSP's successfully gapped: 9
Length of query: 271
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 187
Effective length of database: 1,222,130
Effective search space: 228538310
Effective search space used: 228538310
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)