BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0098.0036.p (284 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 384 e-108 gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 193 1e-50 gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 73 2e-14 gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 62 5e-11 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 384 bits (986), Expect = e-108, Method: Compositional matrix adjust. Identities = 207/280 (73%), Positives = 235/280 (83%), Gaps = 6/280 (2%) Query: 10 GKGYYTVKKGDTLYRIALESGQSYRDIVAWNNLTNPNDIKVDQVLRVAPPDGVQTGAVVA 69 G+GYYTVKKGDTLYRIALE+GQ++RDIVAWNNLTNPNDIKVDQ LRVAPPD A A Sbjct: 54 GRGYYTVKKGDTLYRIALEAGQNWRDIVAWNNLTNPNDIKVDQTLRVAPPDRAPGTAQTA 113 Query: 70 PAAGAS--GIEVRPLGA-STSAGAAPAATGANKTGPRGDKRAYSDSTLAELEKPDA--AP 124 A + G+EV+PLGA TSA AAP G+NKTGPRGDKR YS+S LAELEKPDA AP Sbjct: 114 AIAASPAAGLEVKPLGAPGTSAPAAPVPVGSNKTGPRGDKRPYSESALAELEKPDAPSAP 173 Query: 125 SSAATAPAAAPAATAQASAKPDAAHPAEKPAVAAAAADDDAVSWMWPADGKVVGTFDGGK 184 +++ T+ A+A + + +P AA A + AAADD+ VSWMWPA+GKVVGTF+GGK Sbjct: 174 AASGTSVASAAPSAPAKAEQPHAADKPAA-AASQAAADDEGVSWMWPAEGKVVGTFEGGK 232 Query: 185 KGVDIAGKSGQPVLAAGAGKVMYAGAGIRGYGNLVIVKHTNNLLSAYAHNKTILVKEGQA 244 KGVDIAGK+GQ VLAAGAGKVMYAG+GIRGYGNLVI+KHT+NLLSAYAHN+TILVKEGQ+ Sbjct: 233 KGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLVIIKHTSNLLSAYAHNQTILVKEGQS 292 Query: 245 VTKGQKIAEMGNSDSDSVKLHFEIRQQGKPVDPSKYLPPR 284 VTKGQKIAEMG SDSD+VKLHFEIRQQGKPVDPSKYLPPR Sbjct: 293 VTKGQKIAEMGKSDSDTVKLHFEIRQQGKPVDPSKYLPPR 332 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 193 bits (491), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 122/273 (44%), Positives = 150/273 (54%), Gaps = 50/273 (18%) Query: 12 GYYTVKKGDTLYRIALESGQSYRDIVAWNNLTNPNDIKVDQVLRVAPPDGVQTGAVVAPA 71 GYYTVK+GDTLY I + QS ++ WN L NP+D++V Q + V PP GV Sbjct: 32 GYYTVKRGDTLYSIGRDFDQSPANLRTWNRLRNPDDLEVGQRILVRPPVGVV-------- 83 Query: 72 AGASGIEVRPLGASTSAGAAPAATGANKTGPRGDKRAYSDSTLAELEKPDAAPSSAATAP 131 A T AG +P ++ ++ + KP APS P Sbjct: 84 ------------ARTGAGQSPRPAASSS----------PSASGSGSRKP--APSRPTAKP 119 Query: 132 AAAPAATAQASAKPDAAHPAEKPAVAAAAADDDAVSWMWPADGKVVGTFDGGKKGVDIAG 191 AA P + PA A A D W WP GK + D KKG+DI G Sbjct: 120 AAKP-------------QDDDAPAGAEAKVD-----WSWPTAGKSAPSADSYKKGIDIIG 161 Query: 192 KSGQPVLAAGAGKVMYAGAGIRGYGNLVIVKHTNNLLSAYAHNKTILVKEGQAVTKGQKI 251 GQ V+AA AGKV YAG GIRGYGN+VI+KHT LLS YAHNKTILVKEGQ VT+GQ+I Sbjct: 162 SEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVYAHNKTILVKEGQTVTRGQQI 221 Query: 252 AEMGNSDSDSVKLHFEIRQQGKPVDPSKYLPPR 284 A MGNSD++ VKL+FEIR+ GKP++ LP R Sbjct: 222 ATMGNSDTNKVKLYFEIRRNGKPINVMAVLPAR 254 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 73.2 bits (178), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 43/121 (35%), Positives = 67/121 (55%), Gaps = 8/121 (6%) Query: 171 PADGKVVGTF-----DG-GKKGVDIAGKSGQPVLAAGAGKVMYAGAGIRGYGNLVIVKHT 224 P G ++ F DG +G+ I G V A AG+V++A +RG+GNL+IV H Sbjct: 351 PVKGDLMAKFGSRRGDGPTSRGLFIRAHEGAEVRAVAAGRVVFAD-WLRGFGNLIIVDHG 409 Query: 225 NNLLSAYAHNKTILVKEGQAVTKGQKIAEMGNS-DSDSVKLHFEIRQQGKPVDPSKYLPP 283 N ++ Y +N+++L + G V G IA GNS ++ L+FE+R QG+ DP ++ Sbjct: 410 NQYMTIYGNNQSVLKRAGDLVKAGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVTS 469 Query: 284 R 284 R Sbjct: 470 R 470 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 62.0 bits (149), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 27/68 (39%), Positives = 44/68 (64%), Gaps = 1/68 (1%) Query: 215 YGNLVIVKHTNNLLSAYAHNKTILVKEGQAVTKGQKIAEMGNS-DSDSVKLHFEIRQQGK 273 +GN++ + H NN+++ YAH + VK G V +GQ +A++G++ S LHFE+R +G Sbjct: 238 FGNMLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGI 297 Query: 274 PVDPSKYL 281 DP K+L Sbjct: 298 AQDPRKFL 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.309 0.127 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 722,657 Number of extensions: 26471 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 5 Length of query: 284 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 200 Effective length of database: 1,222,130 Effective search space: 244426000 Effective search space used: 244426000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 130 (54.7 bits)