BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0098.0010.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 444 e-126 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 283 1e-77 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 132 3e-32 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 108 7e-25 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 64 2e-11 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 444 bits (1143), Expect = e-126, Method: Compositional matrix adjust. Identities = 240/293 (81%), Positives = 263/293 (89%) Query: 1 MPIALWALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTA 60 MPIALWAL ISAFAIGTTEFVIMGLLPEVAANLG +LSSAGLLVTGYALGVFVGAPL+TA Sbjct: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA 60 Query: 61 TTGKLPRKTLLVSLMVLFVIGNVGCAIAPDYATLMISRIVASFSHGAFFGVGSVVATGLV 120 TTG+LPRKTLLV+LMV+FV+GN+ CAIAPDY TLMI R+VASF+HGAFFGVGSVVAT LV Sbjct: 61 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV 120 Query: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQAYGWRSTFWAVSAIGVVALLATAYLVPR 180 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQ+YGWR+TFWAV+ IGV+AL ATA LVPR Sbjct: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 Query: 181 SDDRQDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQAGAVSPI 240 S ++ DF E+RVL RPQV+LGLLMTVLGFGGVFAAFTYIAPILTD++GF A AVSPI Sbjct: 181 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI 240 Query: 241 LLLFGVGLVGGNLLGGKFADKYLMPTLVVSLVLLAGVLVLFSFTSHYKLAAVI 293 LLLFG+GLV GNL GGK ADK LM TL+ SLVLLA VLVLF T K+AA+I Sbjct: 241 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAII 293 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 283 bits (724), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 157/292 (53%), Positives = 201/292 (68%) Query: 5 LWALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGK 64 L+ALA++AF IGTTEFVIMGLLP+VA +LG T +AG+LV+GYALGV GAP++ T K Sbjct: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 Query: 65 LPRKTLLVSLMVLFVIGNVGCAIAPDYATLMISRIVASFSHGAFFGVGSVVATGLVPKEK 124 PRK LV LM LF++GNV CA+AP+Y LM++R+V +F H AFFG+GSVVA LV +K Sbjct: 65 WPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQK 124 Query: 125 QASAIALMFTGLTVANVLGVPFGTWLGQAYGWRSTFWAVSAIGVVALLATAYLVPRSDDR 184 +A A+ALMFTGLTVAN+LGVP GT G A GWRSTF AV+AIGV+A + ++P+ Sbjct: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPM 184 Query: 185 QDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQAGAVSPILLLF 244 Q + E R + +V L LL + L +F+ TYIA IL D+TGF S +LLLF Sbjct: 185 QAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWVLLLF 244 Query: 245 GVGLVGGNLLGGKFADKYLMPTLVVSLVLLAGVLVLFSFTSHYKLAAVISFF 296 G G+ G L+GGK AD LMP + + +A VL LFS TSHYK + + F Sbjct: 245 GAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMF 296 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 132 bits (333), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 91/270 (33%), Positives = 149/270 (55%), Gaps = 3/270 (1%) Query: 8 LAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLPR 67 LA+ FAIGT F+I LLP +AA+ G ++ LV + L + + +P+ T + + R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KTLLVSLMVLFVIGNVGCAIAPDYATLMISRIVASFSHGAFFGVGSVVATGLVPKEKQAS 127 + +L++ M F N+ A + + L+++R++ + + G + S +A +V ++ Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AIALMFTGLTVANVLGVPFGTWLGQAYGWRSTFWAVSAIGVVALLATAYLVPRS--DDRQ 185 A+A++ G+T + LG+P G +G A+GWR+TF AVSA+G VAL VPR Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 186 DADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQAGAVSPILLLFG 245 A R + V+G+PQV L +T+L G +AA+ YIAP L+ + F +S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 246 VGLVGGNLLGGKFADKYLMPTLV-VSLVLL 274 G +GG D+Y +V VSL+LL Sbjct: 269 AAAAVGVSVGGTLNDRYGSARVVRVSLLLL 298 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 108 bits (269), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 75/236 (31%), Positives = 127/236 (53%) Query: 7 ALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLP 66 +LA+ F++ T EF+ LL +A++L + +AG VT A+ V AP + T + Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 67 RKTLLVSLMVLFVIGNVGCAIAPDYATLMISRIVASFSHGAFFGVGSVVATGLVPKEKQA 126 RK +++ L +L ++ N+ AP+ L+++R++ + G F+ + + +A LVP A Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQAYGWRSTFWAVSAIGVVALLATAYLVPRSDDRQD 186 A++L+ TG++VA V P G ++G +GWRS F A + +VA+ +PR + Sbjct: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 Query: 187 ADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQAGAVSPILL 242 + +LGR V + LL +L G FA FTYI P++ IT +S +LL Sbjct: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLL 276 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 63/260 (24%), Positives = 108/260 (41%), Gaps = 7/260 (2%) Query: 8 LAISAFAIGTTEFVIMGLLPE-VAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLP 66 LA FA+ +F++M L + ++ T GL+V+ YA L + Sbjct: 27 LAFLQFAV-ILDFMLMSPLGALIMPSMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFD 85 Query: 67 RKTLLVSLMVLFVIGNVGCAIAPDYATLMISRIVASFSHGAFFGVGSVVATGLVPKEKQA 126 RK LL+ FV+G V C +A + +L+++RIV G + +AT L + Sbjct: 86 RKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRG 145 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQAYGWRSTFWAVSAIGVVALLATAYLVPRSD---- 182 + ++ T + VLG+P G +L + W F A++A+G+ L + D Sbjct: 146 RVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGLAGGLVVLVRMQPVDGHLT 205 Query: 183 -DRQDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQAGAVSPIL 241 ++ + + L P+ L T L G F + + L G + I Sbjct: 206 LKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIY 265 Query: 242 LLFGVGLVGGNLLGGKFADK 261 L+ G+ + L G+ ADK Sbjct: 266 LVTGLCTIAFGPLIGRAADK 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 888,981 Number of extensions: 36279 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 301 Number of HSP's successfully gapped: 12 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)