BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0095.0016.p (1157 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspi... 214 2e-56 gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspi... 193 6e-50 >gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1291 Score = 214 bits (546), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 182/645 (28%), Positives = 291/645 (45%), Gaps = 57/645 (8%) Query: 453 DGHQALKSYLMLAEPPRSDTAFLTPQLIRRW----QLDAHLLAGEQR-DLGERLLTFYSN 507 D + ALK+YLMLA+ R++++ L Q+ R W + + + EQ ERL++F Sbjct: 603 DAYNALKTYLMLADKSRAESSHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFSLE 662 Query: 508 HLAAHPDW-KITPSAGLVQAARQTLLAAIGVQHADTTLYRHIVTAATDKYPDQTLASLTV 566 + P W I LV AR+ L + A +Y + A ++ T+A + V Sbjct: 663 QIN-DPSWPTIDNKLTLVDQARENLRRVVRGMPARERVYADVKARAATRFASMTVARI-V 720 Query: 567 GTDPRGLIRNHAIVSGVFTRQAYEGAIAPAIVEAAKRNTVARDWVLTGQAQPATQTPDQS 626 G R L+ + G FTR A+E + A EAA + + DWVL A+ T + S Sbjct: 721 GDKDRELVMGSYAIPGTFTRDAWEKFVQEAFKEAANKELQSADWVLKTSAKDDL-TLEGS 779 Query: 627 PQALQAALTAQYFAEYAEQWQMFMNNVQWEAAPTMPAAIEQLKLMTDARQSPVIALMKSL 686 P+ +Q AL QY EYA +WQ F+ V + A + + D SP+ ++ ++ Sbjct: 780 PEQIQKALVTQYKTEYAREWQKFLQGVSIVELRNLDDATNAMNRLGDPITSPMKKVINTV 839 Query: 687 QYQGTAGVRQASLSDTLVAKAQD---------LLTATKATTPPALQND-----PAGPLGS 732 Y T+ SL D +A A+ +L + A P D P GP+G Sbjct: 840 -YDETSW-DNPSLVDAGLANAKSGFMGWFKETILRRSPAPAPTQFNADGTTAIPMGPVGR 897 Query: 733 TFSPVLRLIGNADGNGNGNGNGNGGADSTNTHVPANSDLSLQRYLDRISALRLRLQQINS 792 FS V RL+ + D NG+ LQ YLD +S LR RL I + Sbjct: 898 EFSGVARLVVSKDKNGS----------------------LLQTYLDNLSKLRTRLNTIKN 935 Query: 793 SPDAEAQAKQLALSLFQGKGSELADTQAYA-QLVAASLGEQWASMGDALFVRPVVQATQT 851 D AKQL +G GSEL+D+ + + + L +Q ++ L VRP+VQ Sbjct: 936 QGDTGPGAKQLMQQTLEGNGSELSDSLKFVDEEMLPGLNDQQKTVLRPLLVRPLVQGFNA 995 Query: 852 VLQPAQAGLNEAWNNTVAAAWYKAFSGRYPFA-QTDNDASLPELARFLRPQSGLIASFLQ 910 +++P + +N+ W V + + +YPF+ + +A+ E+ ++G I+ F+ Sbjct: 996 LVRPTEGEVNKIWRAQVYEPFQSNLAAKYPFSPNSKIEANSGEIGTVF-GENGAISKFVT 1054 Query: 911 SQLAGVLELQADQWVPH--AGSKLNFDP----GFLAAINTLQRLGARLLVQGEPQYRFEL 964 + + ++ + D P A +N P F + I+ G Q F++ Sbjct: 1055 TAMGPLVVRRGDTLSPRKWADMGINLSPAITSNFASWISAPGGGGDANGGANSAQTVFQI 1114 Query: 965 KPMPTPGIEDSTLIIDAQKLRYRNGPQTWQNLVWPVNQTQDAGTLLQWQTDQAGTNKHYE 1024 +P P PG + T+ ID Q+LRYRN W N +WP N G + QT T + Sbjct: 1115 QPQPAPGALEYTIEIDGQQLRYRNTQAQWTNFIWP-NPQGTPGARVTAQTYDGRTIEVAS 1173 Query: 1025 FSGRWALVRLLERARIEPIDSATYQLTWQATPDVPVISAPVISSA 1069 GR+ L RL+ A+ + D+ ++L+W V++ +ISSA Sbjct: 1174 QPGRFGLERLINSAQRKRKDNGVFELSWTNEGVTVVVNLKIISSA 1218 >gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1266 Score = 193 bits (491), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 198/767 (25%), Positives = 325/767 (42%), Gaps = 88/767 (11%) Query: 361 KKPPQVASRLRALLNVQQEISRFEYRTAHHAPLLTRFGLNRDKEILQALWAPYREASQHL 420 +K + SR AL +Q I + + R H P+ GL + + + L Y + + Sbjct: 483 EKRLDLQSRFEALEILQDRIEQLD-RYRSHRPVSIDLGLYQGDLLERKLREEYFAGIKEI 541 Query: 421 LVAPVQQMLETEXPGCQLRSDQLDK-----QAGSFAL----------------------- 452 ++ PV +E S +L+ QA S A Sbjct: 542 MLKPVASSIENYLIEVNANSSKLEPMSRPPQADSLATTGDNNIHAGNALRTYKDSSATNV 601 Query: 453 -DGHQALKSYLMLAEPPRSDTAFLTPQLIRRW----QLDAHLLAGEQR-DLGERLLTFYS 506 D + ALK+YLML++ R++ + L Q+ R W + + + EQ ERL++F Sbjct: 602 EDAYNALKTYLMLSDKSRAEASHLNDQITRFWRGWLETNRGTMPREQMIRSAERLISFSL 661 Query: 507 NHLAAHPDWKITPSA-GLVQAARQTLLAAIGVQHADTTLYRHIVTAATDKYPDQTLASLT 565 + P W T + LV R+ L + A +Y + A ++ T+A + Sbjct: 662 EQID-DPSWPTTENKLTLVDQTRENLRHVVRGMPARERVYADVKARAATRFASMTVARI- 719 Query: 566 VGTDPRGLIRNHAIVSGVFTRQAYEGAIAPAIVEAAKRNTVARDWVLTGQAQPATQTPDQ 625 VG R L+ + G FTR A+E + A EAA + + DWVL ++ T + Sbjct: 720 VGDKDRELVMGSYAIPGTFTRNAWEKFVEDAFKEAANKELQSADWVLKTSSKEDL-TLEG 778 Query: 626 SPQALQAALTAQYFAEYAEQWQMFMNNVQWEAAPTMPAAIEQLKLMTDARQSPVIALMKS 685 SP+ +Q AL +QY +EYA +WQ F+ V + A + + D SP+ ++ + Sbjct: 779 SPEQIQKALVSQYKSEYAREWQKFLQGVSIVELHNLDDATNAMNRLGDPLTSPINKVIST 838 Query: 686 LQYQGTAGVRQASLSDTLVAKAQDLLTATKATT----------PPALQND-----PAGPL 730 + Y T+ + + L ++ L K T PP L D P GP+ Sbjct: 839 V-YDETSWDNPSLIGSGLATASRGLSGWFKETVLRRSPAPPPAPPQLNTDGGAAIPMGPV 897 Query: 731 GSTFSPVLRLIGNADGNGNGNGNGNGGADSTNTHVPANSDLSLQR-YLDRISALRLRLQQ 789 F+ V RL+ + D GN SL R Y++ +S LR RL Sbjct: 898 AREFAGVARLVVSKD-------KGN----------------SLMRGYMENLSKLRTRLNT 934 Query: 790 INSSPDAEAQAKQLALSLFQGKGSELADTQAYA-QLVAASLGEQWASMGDALFVRPVVQA 848 I + D AKQL G GSEL+D + + + L +Q + L VRP+VQ Sbjct: 935 IKNQGDTGPGAKQLMQQTLDGSGSELSDALKFVDEEMLPGLNDQQKTTLRPLLVRPLVQG 994 Query: 849 TQTVLQPAQAGLNEAWNNTVAAAWYKAFSGRYPFAQTDN-DASLPELARFLRPQSGLIAS 907 ++ P + LN+ W V + + +YPF+ + +A+ E+ +SG I+ Sbjct: 995 FNALVHPTEGELNKIWRAQVYEPFQNNLATKYPFSPSSKIEANSGEINTVF-GESGAISK 1053 Query: 908 FLQSQLAGVLELQADQWVPH--AGSKLNFDPGFLAAI-NTLQRLGA--RLLVQGEPQYRF 962 F+ + + ++ + D P A + P + + + G+ G+ Q F Sbjct: 1054 FVTTAMGPLVVRRGDTLSPRKWADMGITLSPAITTSFADWISTHGSTGAGGGAGDAQTVF 1113 Query: 963 ELKPMPTPGIEDSTLIIDAQKLRYRNGPQTWQNLVWPVNQTQDAGTLLQWQTDQAGTNKH 1022 +++P P PG + T+ ID Q+LRYRN W N VWP N G + T T + Sbjct: 1114 QIQPQPAPGALEYTIEIDGQQLRYRNTQAQWVNFVWP-NPQGSPGARVTAVTYDGRTVEV 1172 Query: 1023 YEFSGRWALVRLLERARIEPIDSATYQLTWQATPDVPVISAPVISSA 1069 GR+ L RL+ A+ + D+ ++L+W ++S +ISSA Sbjct: 1173 ASQPGRFGLERLINTAQRKRKDNGAFELSWTNEGVTVMVSLKIISSA 1219 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,673,028 Number of extensions: 156194 Number of successful extensions: 488 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 473 Number of HSP's successfully gapped: 2 Length of query: 1157 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1061 Effective length of database: 1,165,310 Effective search space: 1236393910 Effective search space used: 1236393910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 136 (57.0 bits)