BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0004.p (286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 86 3e-18 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 86 5e-18 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 77 1e-15 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 71 9e-14 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 69 4e-13 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 85.9 bits (211), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 55/148 (37%), Positives = 76/148 (51%), Gaps = 3/148 (2%) Query: 84 YINGEWLQGAS--VNKNINPSDLSDVIGEYAQADAAQADIAIAAAHAAFPAWSTGNIQAR 141 +INGEW AS INP+ +VIG+ A AD A D A+ AA F W + R Sbjct: 8 FINGEWTASASGRTIDVINPAT-EEVIGKIAHADRADLDRALEAASKGFETWRNMSAFER 66 Query: 142 SDALDKIGSEILARKEELGRLLSREEGKTLAEGIGEAGRAGNIFKFFAAEVLRIQGQKLA 201 S + + + R EE+ RL++ E+GK LAE E A + +FA E R G+ + Sbjct: 67 SKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVP 126 Query: 202 SVRPGMDIEITREPVGVVGIIAPWNFPM 229 + PG + +EPVG V PWNFP+ Sbjct: 127 ARVPGAYQMVIKEPVGPVAAFTPWNFPL 154 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 61/184 (33%), Positives = 93/184 (50%), Gaps = 11/184 (5%) Query: 84 YINGEWL---QGASVNKNINPSDLSDVIGEYAQADAAQADIAIAAAHAAFPAWSTGNIQA 140 ++NG+W QG + + NP++ +++G AA+ AI AA+AA+PAW + Sbjct: 15 FVNGQWCDADQGERLAVH-NPAN-GELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Query: 141 RSDALDKIGSEILARKEELGRLLSREEGKTLAEGIGEAGRAGNIFKFFAAEVLRIQGQKL 200 RS L + +LA ++L +++ E+GK LAE GE G A + ++FA E R G + Sbjct: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 132 Query: 201 ASVRPGMDIEITREPVGVVGIIAPWNFPMAIPAWKIAPXTSTSPSRQPCKARSSRPANAA 260 S P I + +E +GV I PWNFP A+ K P + C +PA A Sbjct: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAG-----CP-MVLKPAEAT 186 Query: 261 PLRA 264 P A Sbjct: 187 PFSA 190 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 46/111 (41%), Positives = 62/111 (55%), Gaps = 6/111 (5%) Query: 152 ILARKEELGRLLSREEGKTLAEGIGEAGRAGNIFKFFAAEVLRIQGQKLASVRPGMDIEI 211 +LA +++LGRL+SRE+GK LAE GE A + ++F E R G + + G + Sbjct: 86 LLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPAPVTGRRMMA 145 Query: 212 TREPVGVVGIIAPWNFPMAIPAWKIAPXTSTSPSRQPCKARSSRPANAAPL 262 +EPVGVV I PWNFP A+ A KIAP + + CK PA PL Sbjct: 146 LKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVV-CK-----PAEDTPL 190 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 71.2 bits (173), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 41/129 (31%), Positives = 64/129 (49%) Query: 108 IGEYAQADAAQADIAIAAAHAAFPAWSTGNIQARSDALDKIGSEILARKEELGRLLSREE 167 + + A + + D A+ A AAFPAWS R+ L + I R ++ R++ RE Sbjct: 32 VAQVALGTSDEVDAAVKAGVAAFPAWSNTPPLTRARVLFRFLHLIQQRADDFARIIVREH 91 Query: 168 GKTLAEGIGEAGRAGNIFKFFAAEVLRIQGQKLASVRPGMDIEITREPVGVVGIIAPWNF 227 GKT ++ GE R I +F A ++G+ + G+D R+ +GVV I P+NF Sbjct: 92 GKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQIARGIDAWSMRQALGVVAGITPFNF 151 Query: 228 PMAIPAWKI 236 P +P W Sbjct: 152 PAMVPMWMF 160 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 69.3 bits (168), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 56/176 (31%), Positives = 84/176 (47%), Gaps = 28/176 (15%) Query: 68 GSFXPPQVSTAAGFANYINGEWLQGASVNKNINPSDLSDVIG----EYAQADAAQADIAI 123 G F PP + GE+ + NI+P VIG E A++ A ++A+ Sbjct: 25 GKFVPP-----------VKGEYFE------NISP-----VIGRAFCEVARSSAEDVELAL 62 Query: 124 AAAHAAFPAWSTGNIQARSDALDKIGSEILARKEELGRLLSREEGKTLAEGIG-EAGRAG 182 AAHAA +W + R++ L KI + A E L + + GK + E + + A Sbjct: 63 DAAHAAKKSWGKTSPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAI 122 Query: 183 NIFKFFAAEVLRIQGQKLASVRPGMDIEITREPVGVVGIIAPWNFPMAIPAWKIAP 238 + F++FAA V R Q + + EP+GVVG I PWNFP+ + WK+AP Sbjct: 123 DHFRYFAAAV-RTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAP 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 652,383 Number of extensions: 22300 Number of successful extensions: 129 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 15 Length of query: 286 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 202 Effective length of database: 1,222,130 Effective search space: 246870260 Effective search space used: 246870260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)