BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0003.p (182 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 97 1e-21 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 96 1e-21 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 75 2e-15 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 75 4e-15 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 68 4e-13 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 96.7 bits (239), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 47/113 (41%), Positives = 72/113 (63%), Gaps = 2/113 (1%) Query: 1 VVDQSQLDQNLSYIEIGKQEGASLVSGGERIKRDTDGFYMTPALFADCNNDMRISREEIF 60 V ++Q ++ YI+ G EGA+L++GG + R G+++ P +FAD NN M I+REEIF Sbjct: 331 VAKKAQFERVNRYIQQGLDEGATLLAGG--VSRPDKGWFVQPTIFADANNGMSIAREEIF 388 Query: 61 GPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRHAQAGMVMVN 113 GPV ++I D E A+ LAND+++GL++ + TT+L A + G V VN Sbjct: 389 GPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVN 441 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 96.3 bits (238), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 54/113 (47%), Positives = 70/113 (61%), Gaps = 4/113 (3%) Query: 4 QSQLDQNLSYIEIGKQEGASLVSGGERIKRDTD---GFYMTPALFADCNNDMRISREEIF 60 Q QL++ LSYI+IGKQEGA +++GG R + D G+Y+ P +F NN MRI +EEIF Sbjct: 346 QEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPTVF-QGNNKMRIFQEEIF 404 Query: 61 GPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRHAQAGMVMVN 113 GPV SV + E AL +ANDT +GL +G+ T A R QAG V N Sbjct: 405 GPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTN 457 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 75.5 bits (184), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 37/98 (37%), Positives = 56/98 (57%), Gaps = 2/98 (2%) Query: 17 GKQEGASLVSGGERIKRDTDGFYMTPALFADCNNDMRISREEIFGPVASVIPADNYEHAL 76 G + GA+L +G R + G+++ P L A +N M ++R EIFGPVA IP ++ E A+ Sbjct: 357 GLEAGATLAAGHTRYGEE--GYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAI 414 Query: 77 QLANDTEFGLSSGICTTSLKHATHFKRHAQAGMVMVNT 114 +AND+EFGL+ + T + A AG VN+ Sbjct: 415 AIANDSEFGLAGAVWTRDVARAHRVAASVNAGTFWVNS 452 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 74.7 bits (182), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 39/114 (34%), Positives = 64/114 (56%), Gaps = 1/114 (0%) Query: 1 VVDQSQLDQNLSYIEIGKQEGASLVSGGE-RIKRDTDGFYMTPALFADCNNDMRISREEI 59 + +++Q + IE G Q+GA L++GG R G+Y P +F++ + DM I++EEI Sbjct: 332 LANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPTIFSEVHTDMVIAQEEI 391 Query: 60 FGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRHAQAGMVMVN 113 FGPV +++P D E A+ +ANDT +GL + + Q+G V +N Sbjct: 392 FGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQSGQVHLN 445 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 68.2 bits (165), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 36/125 (28%), Positives = 67/125 (53%), Gaps = 1/125 (0%) Query: 1 VVDQSQLDQNLSYIEIGKQEGASLVSGGE-RIKRDTDGFYMTPALFADCNNDMRISREEI 59 ++ ++Q ++ ++ +++G +L + G+ T GFY PAL D R+++EE+ Sbjct: 321 LIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFYQAPALLRDVPPRHRLAQEEV 380 Query: 60 FGPVASVIPADNYEHALQLANDTEFGLSSGICTTSLKHATHFKRHAQAGMVMVNTATAGV 119 FGPV + +P ++ HA+ LAN T++GL + + T R ++G V +N AG Sbjct: 381 FGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQMRLARAIRSGQVFINNYGAGG 440 Query: 120 DSQQP 124 + P Sbjct: 441 GVELP 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 555,121 Number of extensions: 21087 Number of successful extensions: 76 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 72 Number of HSP's successfully gapped: 11 Length of query: 182 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 102 Effective length of database: 1,241,070 Effective search space: 126589140 Effective search space used: 126589140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)