BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0094.0002.p (345 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 379 e-106 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 369 e-103 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 369 e-103 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 363 e-102 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 353 8e-99 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 379 bits (973), Expect = e-106, Method: Compositional matrix adjust. Identities = 210/341 (61%), Positives = 256/341 (75%) Query: 3 KFFELIAENKSQEARDIYQGDMARTQAAYYVLVDKMVDYQAKEMENDVINAAVQARNAKI 62 K ELI + K +EA ++ + Q Y+ ++D+ V +Q ME V ++A Sbjct: 131 KLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEGRDTTQSAIA 190 Query: 63 QMAILSACAVMLSIVTGIFITRTITRPINRAVDLAEAVAQGNLTHHLQIDSKDEIGRLLV 122 M LSA A+ L ++ +TR+ITRP+N AV +A AVA G+LT + SKDE G LL Sbjct: 191 MMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGATSKDETGALLA 250 Query: 123 ALKIMIENLHGIVSNVRKGTDTIAEASREVASGNMDLSSRTEQQASSLEETASAMEQLTS 182 ALK M ++LH IVS VR+G+DTI AS E+A+GN+DLSSRTE+QA +LEETASAME+LTS Sbjct: 251 ALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEETASAMEELTS 310 Query: 183 AVRQNADNARQANQLASNASAVAVKGGDAVGQVVDTMTAINNSSRKIVDIIGVIDGIAFQ 242 V+QNADNARQAN LA+ AS VAV+GG VGQVV TM IN++SRKIVDII VIDGIAFQ Sbjct: 311 TVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDIISVIDGIAFQ 370 Query: 243 TNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHSVNQVGNGSKM 302 TNILALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK LID SV++V NGS++ Sbjct: 371 TNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALIDDSVSRVDNGSRL 430 Query: 303 VEQAGQTIQDVVTSIRHVTDIVAEISSSSQEQSIGIEQINQ 343 VEQAG T+ +VV S+R VTD+VAEIS++S EQS GIEQIN Sbjct: 431 VEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINH 471 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 369 bits (948), Expect = e-103, Method: Compositional matrix adjust. Identities = 201/313 (64%), Positives = 250/313 (79%) Query: 30 AYYVLVDKMVDYQAKEMENDVINAAVQARNAKIQMAILSACAVMLSIVTGIFITRTITRP 89 AY + K+VD+Q KEM++ A ++ + +L ++L IV ++T IT+P Sbjct: 159 AYQDTMRKLVDFQRKEMDDTAREIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKP 218 Query: 90 INRAVDLAEAVAQGNLTHHLQIDSKDEIGRLLVALKIMIENLHGIVSNVRKGTDTIAEAS 149 ++RAVD++ VA+G+L+ + + S+DE G+LL +LK M ++L GIVSNVR GTDTI+ AS Sbjct: 219 LHRAVDVSRCVAEGDLSSEIAVTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTAS 278 Query: 150 REVASGNMDLSSRTEQQASSLEETASAMEQLTSAVRQNADNARQANQLASNASAVAVKGG 209 E+A+GN+DLSSRTEQQASSL ETASAME+L S VRQNADNARQA+QLA++ASAVA +GG Sbjct: 279 SEIAAGNLDLSSRTEQQASSLAETASAMEELISTVRQNADNARQASQLANSASAVAEQGG 338 Query: 210 DAVGQVVDTMTAINNSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEHGRGFAVVAS 269 V QVVDTM AIN SSRKIVDII VIDGIAFQTNILALNAAVEAARAGE GRGFAVVAS Sbjct: 339 GVVSQVVDTMGAINASSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 398 Query: 270 EVRNLAQRSAVAAKEIKTLIDHSVNQVGNGSKMVEQAGQTIQDVVTSIRHVTDIVAEISS 329 EVR LAQRSA AAKEIK LI+ SV +V +GS++VEQAG T+Q+VV+S+R VTDIVAEIS+ Sbjct: 399 EVRTLAQRSASAAKEIKELINDSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISA 458 Query: 330 SSQEQSIGIEQIN 342 +S EQ+ GIEQIN Sbjct: 459 ASTEQTHGIEQIN 471 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 369 bits (947), Expect = e-103, Method: Compositional matrix adjust. Identities = 210/341 (61%), Positives = 256/341 (75%) Query: 3 KFFELIAENKSQEARDIYQGDMARTQAAYYVLVDKMVDYQAKEMENDVINAAVQARNAKI 62 K +LI + K EA Q Y+ V +MV +Q+ M+ A+ + +A Sbjct: 132 KVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAERASAASSSAIN 191 Query: 63 QMAILSACAVMLSIVTGIFITRTITRPINRAVDLAEAVAQGNLTHHLQIDSKDEIGRLLV 122 M +LS AV+LS + + ITR+ITRP+N AV +A AVA G+LT ++ SKDE G LL Sbjct: 192 IMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEATSKDETGALLG 251 Query: 123 ALKIMIENLHGIVSNVRKGTDTIAEASREVASGNMDLSSRTEQQASSLEETASAMEQLTS 182 ALK M +NLH IV+ VR GTDTI ASRE+A+GN+DLSSRTEQQA +LEETASAME+LTS Sbjct: 252 ALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEETASAMEELTS 311 Query: 183 AVRQNADNARQANQLASNASAVAVKGGDAVGQVVDTMTAINNSSRKIVDIIGVIDGIAFQ 242 VRQNADNARQAN LA AS VA++GG VG+VV TM IN++SRKIVDII VIDGIAFQ Sbjct: 312 TVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDIISVIDGIAFQ 371 Query: 243 TNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHSVNQVGNGSKM 302 TNILALNAAVEAARAGE GRGFAVVASEVR LAQRSA AAKEIK+LID SV +V +GS++ Sbjct: 372 TNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDASVERVESGSRL 431 Query: 303 VEQAGQTIQDVVTSIRHVTDIVAEISSSSQEQSIGIEQINQ 343 V QAG T+++VV S++ VTD+VAEI+++S EQS GIEQINQ Sbjct: 432 VGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQ 472 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 363 bits (933), Expect = e-102, Method: Compositional matrix adjust. Identities = 204/345 (59%), Positives = 255/345 (73%), Gaps = 10/345 (2%) Query: 3 KFFELIAENKSQEARDIYQGDMARTQAAYYVLVDKM--VDYQAKEMEN---DVINAAVQA 57 K L + K +EAR++ +G+ + +DK+ V+ + ++ N D I A Sbjct: 132 KIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISNKQADAIYA---- 187 Query: 58 RNAKIQMAILSACAVMLSIVTGIFITRTITRPINRAVDLAEAVAQGNLTHHLQIDSKDEI 117 A++ + + A + L+++ ++++R I RP+ AV +A+ VA G+LT ++ KDE Sbjct: 188 -QARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEPAGKDET 246 Query: 118 GRLLVALKIMIENLHGIVSNVRKGTDTIAEASREVASGNMDLSSRTEQQASSLEETASAM 177 G+LLVALK M ++L GIV VR GT TI AS E+ASGN+DLSSRTE+QASSLEETASAM Sbjct: 247 GQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLEETASAM 306 Query: 178 EQLTSAVRQNADNARQANQLASNASAVAVKGGDAVGQVVDTMTAINNSSRKIVDIIGVID 237 E+LTS V+QNADNARQANQLA +AS VA +GG V QVVDTM +IN SSRKI DIIGVID Sbjct: 307 EELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRKIADIIGVID 366 Query: 238 GIAFQTNILALNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHSVNQVG 297 GIAFQTNILALNAAVEAARAGE GRGFAVVASEVR+LAQRSA AAKEIK LI+ SV QV Sbjct: 367 GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIEGSVAQVD 426 Query: 298 NGSKMVEQAGQTIQDVVTSIRHVTDIVAEISSSSQEQSIGIEQIN 342 GSK+VE+AG T+ +VV S+R VTDIV EISS+SQEQS GIEQ+N Sbjct: 427 MGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQVN 471 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 353 bits (907), Expect = 8e-99, Method: Compositional matrix adjust. Identities = 193/336 (57%), Positives = 252/336 (75%), Gaps = 2/336 (0%) Query: 9 AENKSQEARDIYQGDMARTQAAYYVLVDKMVDYQ-AKEMENDVINAAVQARNAKIQMAIL 67 AE K +EAR +++GD +T + +D+++ A +D + V R+A + + L Sbjct: 138 AEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDDMAEQVY-RSATLWIIGL 196 Query: 68 SACAVMLSIVTGIFITRTITRPINRAVDLAEAVAQGNLTHHLQIDSKDEIGRLLVALKIM 127 A V++++ + + R ++RP+N AV +AE VAQG+LT ++ S DE G L+ +L+ M Sbjct: 197 LAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRATSTDETGMLMHSLRAM 256 Query: 128 IENLHGIVSNVRKGTDTIAEASREVASGNMDLSSRTEQQASSLEETASAMEQLTSAVRQN 187 ++L IV VR GTDTI AS+E+A+GN+DLSSRTE+QA SLEETASAME+LT+ V+QN Sbjct: 257 NDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLEETASAMEELTATVKQN 316 Query: 188 ADNARQANQLASNASAVAVKGGDAVGQVVDTMTAINNSSRKIVDIIGVIDGIAFQTNILA 247 ADNARQANQLA++AS +A +GG VGQVV TM I SS +I DII VIDGIAFQTNILA Sbjct: 317 ADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIADIISVIDGIAFQTNILA 376 Query: 248 LNAAVEAARAGEHGRGFAVVASEVRNLAQRSAVAAKEIKTLIDHSVNQVGNGSKMVEQAG 307 LNAAVEAARAGE GRGFAVVASEVR+LAQRSA AAKEIK LID SV +VG GS++VE+AG Sbjct: 377 LNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKVGAGSQLVERAG 436 Query: 308 QTIQDVVTSIRHVTDIVAEISSSSQEQSIGIEQINQ 343 T+ +VV S+R VTD+VAEIS++S EQS GIE+IN+ Sbjct: 437 ATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINR 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.322 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 664,629 Number of extensions: 20519 Number of successful extensions: 399 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 71 Length of query: 345 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 259 Effective length of database: 1,212,660 Effective search space: 314078940 Effective search space used: 314078940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)