BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0093.0028.p (105 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 123 4e-30 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 123 bits (308), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 55/88 (62%), Positives = 68/88 (77%) Query: 18 SIARPSRLLETLLANLDGMVYRCRDDDEWTMEFISEGCLELTGYQPEDLLFNHRISYEDI 77 +I R RLLETLL NLDG+VYRCRDD +WTMEF+SEGCL LTGY PEDLL N R+S+ ++ Sbjct: 2 AIPRSPRLLETLLVNLDGVVYRCRDDAQWTMEFVSEGCLALTGYLPEDLLLNQRLSFLEL 61 Query: 78 THPEDRETVRQTIRACLDGKRRFELMTR 105 THPEDR VR + AC+ +RR ++ R Sbjct: 62 THPEDRTLVRNHVDACMRERRRIDIEYR 89 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 300,206 Number of extensions: 10190 Number of successful extensions: 42 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 105 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 33 Effective length of database: 1,278,950 Effective search space: 42205350 Effective search space used: 42205350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)