BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0093.0027.p (362 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 550 e-158 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 346 1e-96 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 271 8e-74 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 266 2e-72 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 259 2e-70 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 550 bits (1418), Expect = e-158, Method: Compositional matrix adjust. Identities = 281/407 (69%), Positives = 309/407 (75%), Gaps = 56/407 (13%) Query: 1 MLHNHLQRAIQSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRET 60 MLH HLQRA++ RQKG LTAV FVDLDQFKFINDSLGHQVGDELLKTVAQRL +C+RE Sbjct: 279 MLHEHLQRALRVARQKGALTAVAFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREA 338 Query: 61 DMVARQGGDEFVLVLQNQPD-ENRITEVLQRILSAVARPWVVGDREFHVTCSIGVSRCPT 119 DMVARQGGDEFVLVLQNQ + E I EV+QRIL+AVARPW GDREF VT SIGVSR P Sbjct: 339 DMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPA 398 Query: 120 DGRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDEFKLY 179 DG+DVE+LLK+ADSAMY+AKE GRNNFQ+FA WMD+QVSNRLEML++LRRALD+DEFKLY Sbjct: 399 DGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLY 458 Query: 180 YQPKISLANGRIIGAEALIRWITPDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACAQNK 239 YQPK+SL +GR+IGAEALIRW +P QGMVPPDRFIPFAEEAGLIVPIGEWVLRTAC QN+ Sbjct: 459 YQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQ 518 Query: 240 AWQDAGLAPIPVAVNLSPRQLDQNLPEFVRQVL----------------------AESCL 277 AWQ AGL PIPVAVNLSPRQL+Q+LP+FV VL AE + Sbjct: 519 AWQQAGLPPIPVAVNLSPRQLNQSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSV 578 Query: 278 A---------------------------------AXKIDKSFVRDIARDTDSAAIVKAVI 304 A A KIDKSFVRDI RD DSAAIVKA+I Sbjct: 579 ATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAII 638 Query: 305 SLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKPMPAEDFT 351 SL HILNLRVIAEGVED+EQ+ FL EN CDEVQGY GKPM FT Sbjct: 639 SLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPMEVAVFT 685 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 346 bits (888), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 194/414 (46%), Positives = 242/414 (58%), Gaps = 56/414 (13%) Query: 1 MLHNHLQRAIQSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRET 60 ML +HL++AI ++ VVFVDLD+FKF+ND+LGH+ GD LL+TVA RL + VRE Sbjct: 532 MLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREA 591 Query: 61 DMVARQGGDEFVLVLQNQPDENRITEVLQRILSAVARPWVVGDREFHVTCSIGVSRCPTD 120 D VAR GGDEF+++L Q + T LQRI+ AVA P +G +TCS+GV+ P D Sbjct: 592 DTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPND 651 Query: 121 GRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDEFKLYY 180 G D ETL+K+AD AMY+ K+ GRNNFQ++ M+ Q RL + LR ALD EF L+Y Sbjct: 652 GTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHY 711 Query: 181 QPKISLANGRIIGAEALIRWITPDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACAQNKA 240 QPK+ LA+GR+IG+EALIRW P GMV P RFI AEE GLI+PIG WVLR AC Q + Sbjct: 712 QPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQ 771 Query: 241 WQDAGLAPIPVAVNLSPRQL--------------DQNL-PEFVRQVLAES---------- 275 WQ G A I V+VNLS Q D L PE + L ES Sbjct: 772 WQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAI 831 Query: 276 -CLAAXK------------------------------IDKSFVRDIARDTDSAAIVKAVI 304 LA K ID+SFVRDI D D AAI ++I Sbjct: 832 GILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASII 891 Query: 305 SLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKPMPAEDFTRRLELEK 358 SL L LRVIAEGVE EQ ++L GCDE+QGYL P+PA+ F + L ++ Sbjct: 892 SLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQILRQDR 945 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 271 bits (692), Expect = 8e-74, Method: Compositional matrix adjust. Identities = 165/419 (39%), Positives = 229/419 (54%), Gaps = 61/419 (14%) Query: 1 MLHNHLQRAI-QSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRE 59 +L + L +AI ++ R++G A++F+DLD FK INDSLGH VGD LL VAQRL S R Sbjct: 279 LLEDRLNQAINKAAREQGHF-ALMFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRA 337 Query: 60 TDMVARQGGDEFVLVLQ-NQPDENRITEVLQRILSAVARPWVVGDREFHVTCSIGVSRCP 118 D VAR GGDEFV+++ N P++ V ++++ AV +P+ V E V+ S+G++ P Sbjct: 338 NDTVARLGGDEFVILVDLNTPEDA--MGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYP 395 Query: 119 TDGRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDEFKL 178 DG L+ NAD+AMY K GRN + +F M+A +L+ L LR AL+R +F+L Sbjct: 396 EDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRL 455 Query: 179 YYQPKISLANGRIIGAEALIRWITPDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACAQN 238 YQPK SL G ++GAEAL+RW P G+V PD+FI AE +GLI+PIG WVL AC Q Sbjct: 456 VYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQM 515 Query: 239 KAWQDAGLAPIPVAVNLSPRQL-DQNLPEFVRQVLAES-----CLAAXKIDKSFVRD--- 289 + W G ++VNLS Q D +L E VR L CL + + + D Sbjct: 516 REWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEA 575 Query: 290 -----------------------------------------------IARDTDSAAIVKA 302 + +D + AAIV A Sbjct: 576 SLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDRGFVRELRQDGEDAAIVSA 635 Query: 303 VISLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKPMPAEDFTRRLELEKKAA 361 +++LG LNLR++AEGVE EQ +FL GCD +QGYL+G+PM E F + + K+A Sbjct: 636 IVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRPMTPELFLQTMRERHKSA 694 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 266 bits (680), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 143/406 (35%), Positives = 229/406 (56%), Gaps = 56/406 (13%) Query: 5 HLQRAIQSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRETDMVA 64 H+ AI + + AV+ +DLD+FK IND+ GH+ G+ +L+ +AQR + +RE D++A Sbjct: 687 HVSNAITHAEHRNGMVAVILMDLDRFKNINDTFGHETGEAVLQEIAQRFRNSLRELDILA 746 Query: 65 RQGGDEFVLVLQNQPDENRITEVLQRILSAVARPWVVGDREFHVTCSIGVSRCPTDGRDV 124 R GGDEF++++ + D ++ E+ Q++L+ +P+++ +E ++ SIG++ P DG + Sbjct: 747 RVGGDEFIVLVDDFDDPVQLGEIAQKLLTEARKPFIIDGQEAMLSVSIGIATYPGDGDNA 806 Query: 125 ETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDEFKLYYQPKI 184 +TL+KNAD AMY+AK G++++++F+ ++ R+ + LRRA++R EF ++YQPKI Sbjct: 807 QTLIKNADIAMYRAKHHGKDDYRFFSDEINTNTVERIALEAELRRAIERAEFVVHYQPKI 866 Query: 185 SLANGRIIGAEALIRWITPDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACAQNKAWQDA 244 LA GRI+GAEAL+RW P +G++PP FI AEE LI IG VL AC + Sbjct: 867 ELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEETRLIDQIGLLVLDVACRDIGLMRQQ 926 Query: 245 GLAPIPVAVNLSPRQL-DQNLPEFVRQVL-----AESCLA-------------------- 278 + +++NL+ Q D+ L + +++V+ SCL Sbjct: 927 QMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTPPSCLEFEITESMVMHNRERAIAIMD 986 Query: 279 ------------------------------AXKIDKSFVRDIARDTDSAAIVKAVISLGH 308 KIDKSF+ DI + +S+AIV+A+I++ H Sbjct: 987 GIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSH 1046 Query: 309 ILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKPMPAEDFTRRL 354 LNL+V+ EGVE ++Q L E G DE QGY KP+P F + L Sbjct: 1047 ALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSKPVPYAQFVQLL 1092 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 259 bits (663), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 154/414 (37%), Positives = 222/414 (53%), Gaps = 63/414 (15%) Query: 1 MLHNHLQRAIQSTRQKGTLTAVVFVDLDQFKFINDSLGHQVGDELLKTVAQRLVSCVRET 60 +L + L++AI + + T A++F+DLD FK +ND++GH+ GD LLK VAQRL++ VRE Sbjct: 612 LLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTLLKQVAQRLLANVREN 671 Query: 61 DMVARQGGDEFVLVLQN-----QPDENRITEVLQRILSAVARPWVVGDREFHVTCSIGVS 115 D VAR GGDEFV+VL+ Q + + ++IL+A+ + + D E+ T SIG + Sbjct: 672 DTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTALGSTYQLDDVEYRTTASIGAT 731 Query: 116 RCPTDGRDVETLLKNADSAMYKAKELGRNNFQYFAGWMDAQVSNRLEMLVSLRRALDRDE 175 V+ L+K AD AMYKAKE GRN ++F M V R + LR+A+D ++ Sbjct: 732 VFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLERATLEAGLRKAIDGNQ 791 Query: 176 FKLYYQPKISLANGRIIGAEALIRWITPDQGMVPPDRFIPFAEEAGLIVPIGEWVLRTAC 235 L+YQP++ + GR+ GAE L+RW P +GMVPP FIP AEE GLI+ +G WVL TAC Sbjct: 792 LLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEETGLILSLGNWVLETAC 850 Query: 236 AQNKAWQ-DAGLAPIPVAVNLS-----------------------PRQLD---------Q 262 Q W +A + +AVN+S PR+L Sbjct: 851 RQLATWAAQPQMADLSIAVNVSAPQLREPDFVQTVLDTIERTGANPRRLKLELTESLLVD 910 Query: 263 NLPEFVRQVLAESC------------------------LAAXKIDKSFVRDIARDTDSAA 298 N+ + ++++ A L KID+SFVRD+ D + A+ Sbjct: 911 NVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFVRDVLIDPNDAS 970 Query: 299 IVKAVISLGHILNLRVIAEGVEDQEQYNFLLENGCDEVQGYLLGKPMPAEDFTR 352 I K +++L L L VIAEGVE Q +FL + GC QGY +P+ E F + Sbjct: 971 IAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPVTVEAFEK 1024 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,113,151 Number of extensions: 46530 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 59 Length of query: 362 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 276 Effective length of database: 1,212,660 Effective search space: 334694160 Effective search space used: 334694160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)