BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0093.0022.p (707 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312898|ref|YP_003776990.1| translation initiation factor I... 1269 0.0 gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 78 2e-15 gi|300309454|ref|YP_003773546.1| GTPase translation elongation f... 64 5e-11 gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein... 64 5e-11 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 62 2e-10 >gi|300312898|ref|YP_003776990.1| translation initiation factor IF-2 [Herbaspirillum seropedicae SmR1] Length = 954 Score = 1269 bits (3285), Expect = 0.0, Method: Compositional matrix adjust. Identities = 657/710 (92%), Positives = 677/710 (95%), Gaps = 4/710 (0%) Query: 1 VEDEVAQIKAMMNAPRRVIKAPEPAPAAATKTSEGTLHKPADK-KPGEKKDDKKAPVAVD 59 VEDEVAQIKAMMNAPRRVIKAPEPA AA KTSEGTLHKPA KPGEKKDDKK VA D Sbjct: 246 VEDEVAQIKAMMNAPRRVIKAPEPA-PAAAKTSEGTLHKPATAAKPGEKKDDKKPAVAAD 304 Query: 60 KKSIKSANVSSTWQDDAKKRGTG-MKTRGNTGTGGRDGWRAGPKGRRNSHHDDH-ESNFQ 117 KKSIKSANVSSTWQDDAKKRGTG MK+RG TG GGRDGWRAGPKGRR+SH DD ESNFQ Sbjct: 305 KKSIKSANVSSTWQDDAKKRGTGGMKSRGATGGGGRDGWRAGPKGRRHSHGDDQRESNFQ 364 Query: 118 APTEAIVKDVHVPETITVAEIAHKMSVKASEVIKHLMKLGQMVTINQVLDQETAMIVVEE 177 PTEA+V DV+VPETITVAE+AHKM+VKASEVIKHLMKLGQMVTINQVLDQETAMIVVEE Sbjct: 365 VPTEAVVHDVYVPETITVAEVAHKMAVKASEVIKHLMKLGQMVTINQVLDQETAMIVVEE 424 Query: 178 MGHRAHAAKLDDPEALLEEGVEHPDAELVARAPVVTVMGHVDHGKTSLLDYIRRAKVASG 237 MGHRAHAAKLDDPEALL EG EH DAE + RAPVVTVMGHVDHGKTSLLDYIRR KVASG Sbjct: 425 MGHRAHAAKLDDPEALLVEGEEHGDAEALPRAPVVTVMGHVDHGKTSLLDYIRRTKVASG 484 Query: 238 EAGGITQHIGAYHVETPRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQT 297 EAGGITQHIGAYHVETPRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQT Sbjct: 485 EAGGITQHIGAYHVETPRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQT 544 Query: 298 KEAIAHAKAGGVPLVVAINKIDKQGANLDRVKQELIAEGVVPEEYGGEAPFISVSAKTGE 357 KEAIAHAKAGGVPLVVAINKIDK GAN DRVKQELIAE VVPEEYGG+APFI VSAKTGE Sbjct: 545 KEAIAHAKAGGVPLVVAINKIDKPGANPDRVKQELIAEEVVPEEYGGDAPFIPVSAKTGE 604 Query: 358 GIDSLLENVLLQAEVLELKAAIDVPAKGLVIEAKLDKGRGPVATILVQSGTLKRGDVVLA 417 GIDSLLENVLLQAEVLELKA ++VPA+GLVIEAKLDKGRGPVATILVQSGTLKRGDVVLA Sbjct: 605 GIDSLLENVLLQAEVLELKAPVEVPARGLVIEAKLDKGRGPVATILVQSGTLKRGDVVLA 664 Query: 418 GSAYGRVRAMLDESGKSITEAGPSIPVEIQGLTEVPSAGEEVLVMSDERKAREIGLFRQG 477 GSAYGRVRAMLDE+GK+I EAGPS+PVEIQGLTEVPSAGEEV+VM+DERKAREIGLFRQG Sbjct: 665 GSAYGRVRAMLDENGKNIAEAGPSLPVEIQGLTEVPSAGEEVVVMTDERKAREIGLFRQG 724 Query: 478 KFRDVKLAKQQAAKLENMFDQMAEGEVKNLPMIIKTDVQGSQEALVQSLQKLSTAEVRVQ 537 KFRDVKLAKQQAAKLENMFDQM EGEVKNLPMIIKTDVQGSQEALVQSLQKLST EVRVQ Sbjct: 725 KFRDVKLAKQQAAKLENMFDQMTEGEVKNLPMIIKTDVQGSQEALVQSLQKLSTNEVRVQ 784 Query: 538 VVHAAVGGISENDVNLAVASKAVIIGFNTRADASARKLAEANGVDIRYYNIIYDAVDEVK 597 VVHAAVGGISENDVNLAVASKAVIIGFNTRADASARKLAEA+GVDIRYYNIIYDAVDEVK Sbjct: 785 VVHAAVGGISENDVNLAVASKAVIIGFNTRADASARKLAEASGVDIRYYNIIYDAVDEVK 844 Query: 598 AAMSGMLAPEKREQALGLVEIRQVFVVSKVGSIAGCYVLEGLVKRGSQVRLLRNNVVLWT 657 AAMSGML+PEKREQALGLVEIRQVFVVSKVGSIAGCYVLEGLVKRGSQVRLLRNNVV+WT Sbjct: 845 AAMSGMLSPEKREQALGLVEIRQVFVVSKVGSIAGCYVLEGLVKRGSQVRLLRNNVVVWT 904 Query: 658 GELDSLKRFKDDVKEVKSGFECGLSLKNYNDIQEGDQLEIFEVQEVARTL 707 GELDSLKRFKDDVKEVKSGFECGLSLK YNDI+ GDQLEIFEVQEVARTL Sbjct: 905 GELDSLKRFKDDVKEVKSGFECGLSLKGYNDIEVGDQLEIFEVQEVARTL 954 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 78.2 bits (191), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 67/230 (29%), Positives = 108/230 (46%), Gaps = 39/230 (16%) Query: 213 TVMGHVDHGKTSLLDYIRRAKVASGEAGGIT------QHIGAYHVETPRGM--------- 257 +++ H+DHGK++L D I + GG++ Q + + +E RG+ Sbjct: 8 SIIAHIDHGKSTLADRIIQL------CGGLSNREMEAQVLDSMDIERERGITIKAQTAAL 61 Query: 258 -----------ITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKA 306 + +DTPGH F+ +R A + +LVV A GV QT A Sbjct: 62 SYKAKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTALD 121 Query: 307 GGVPLVVAINKIDKQGANLDRVKQELIAEGVVPEEYGGEAPFISVSAKTGEGIDSLLENV 366 GV +V +NKID A+ D K E+ E V+ + P SAKTG G++ +LE + Sbjct: 122 LGVEVVPVLNKIDLPSADPDNAKSEI--EDVIGIDASDATP---CSAKTGLGVEDVLEAI 176 Query: 367 LLQAEVLELKAAIDVPAKGLVIEAKLDKGRGPVATILVQSGTLKRGDVVL 416 + A+V K + P + L+I++ D G V + + +GTL+ D +L Sbjct: 177 V--AKVPAPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIL 224 >gi|300309454|ref|YP_003773546.1| GTPase translation elongation factor TU [Herbaspirillum seropedicae SmR1] Length = 396 Score = 63.5 bits (153), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 72/229 (31%), Positives = 99/229 (43%), Gaps = 27/229 (11%) Query: 212 VTVMGHVDHGKTSLLDYIRR--AKVASGEAG--------------GITQHIGAYHVETPR 255 V +GHVDHGKT+L I +K GEA GIT + ET Sbjct: 15 VGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETAN 74 Query: 256 GMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVP-LVVA 314 +D PGH + GA D ILV +A DG MPQT+E I ++ GVP ++V Sbjct: 75 RHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVF 134 Query: 315 INKIDK-QGANLDRVKQELIAEGVVPEEY-GGEAPFISVSAKTG-EGIDSLLENVLLQAE 371 +NK D A L + + + E + E+ G + P + SAK EG L + A Sbjct: 135 LNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALEGDTGPLGEQAIMAL 194 Query: 372 VLELKAAIDVPAKGL-------VIEAKLDKGRGPVATILVQSGTLKRGD 413 L I P + + V + GRG V T V+ G +K G+ Sbjct: 195 AEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIKVGE 243 >gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 63.5 bits (153), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 72/229 (31%), Positives = 99/229 (43%), Gaps = 27/229 (11%) Query: 212 VTVMGHVDHGKTSLLDYIRR--AKVASGEAG--------------GITQHIGAYHVETPR 255 V +GHVDHGKT+L I +K GEA GIT + ET Sbjct: 15 VGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETAN 74 Query: 256 GMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVP-LVVA 314 +D PGH + GA D ILV +A DG MPQT+E I ++ GVP ++V Sbjct: 75 RHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVF 134 Query: 315 INKIDK-QGANLDRVKQELIAEGVVPEEY-GGEAPFISVSAKTG-EGIDSLLENVLLQAE 371 +NK D A L + + + E + E+ G + P + SAK EG L + A Sbjct: 135 LNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALEGDTGPLGEQAIMAL 194 Query: 372 VLELKAAIDVPAKGL-------VIEAKLDKGRGPVATILVQSGTLKRGD 413 L I P + + V + GRG V T V+ G +K G+ Sbjct: 195 AEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIKVGE 243 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 62.0 bits (149), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 41/123 (33%), Positives = 59/123 (47%), Gaps = 16/123 (13%) Query: 217 HVDHGKTSLLDYIRRAKVASGEAGGITQHI-GAYHVETPRGM---------------ITF 260 HVDHGKT+L+D + R + + + + +E RG+ I Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVEYKGTHINI 89 Query: 261 LDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVPLVVAINKIDK 320 +DTPGH F R D V+L+V A +G MPQT+ A A G+ +V +NKID+ Sbjct: 90 VDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVVLNKIDR 149 Query: 321 QGA 323 GA Sbjct: 150 PGA 152 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.131 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,897,885 Number of extensions: 77388 Number of successful extensions: 274 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 5 Length of query: 707 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 615 Effective length of database: 1,184,250 Effective search space: 728313750 Effective search space used: 728313750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 134 (56.2 bits)