BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0092.0052.p (496 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 762 0.0 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 387 e-109 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 222 6e-59 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 91 3e-19 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 762 bits (1968), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/492 (80%), Positives = 438/492 (89%), Gaps = 4/492 (0%) Query: 2 MIPAKKILLSSLLSASVLALSPALTGLSTAVAAPAVAGLPDFADIVDRTGPGVVNIRTTE 61 M P K L S+LL+ASV + + + VA+PAVA LPDFADIV+RTGPGVVNIRTTE Sbjct: 1 MTPLKTTLASALLAASVFVAA-PVALAAPPVASPAVAALPDFADIVERTGPGVVNIRTTE 59 Query: 62 RVK-TGQPQAGADDEEMQEFFRRFFGVPMPRQQQPNTPNTPRPNRKGGNAQQEEVPRGVG 120 RV+ Q Q G DD EMQEFFRRFFG+P+PRQQQP TP QQEEVPRGVG Sbjct: 60 RVRQNAQGQGGVDDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGG--QGQQEEVPRGVG 117 Query: 121 SGFIISADGYIMTNAHVVEGASDVYVTLTDKREFKAKIIGSDTRTDVALLKIDGNSLPRL 180 SGFIISADG+IMTNAHVVEGAS+VYVTLTDKREFKAKI+GSDTRTDVA+LKIDG++LPRL Sbjct: 118 SGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRL 177 Query: 181 VIGDSDKIRAGEWVLAIGSPFGLENTVTAGIISAKARDTGDYLPLIQTDVAVNPGNSGGP 240 +GDSDKIR GEWVLAIGSPFGLENTVTAGI+SAKARDTGDYLPLIQTDVAVNPGNSGGP Sbjct: 178 NMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGP 237 Query: 241 LINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVSDQLKASGRVTRGRIGVQIGEVT 300 LINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRV+DQLKASGRVTRGRIGVQIGEVT Sbjct: 238 LINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVT 297 Query: 301 KDVAESLGLAKAQGALVQRIEPGGPAEKGGLEAGDIILKFNGTAIERPSDLPRMVGATKP 360 KDVAESLGLA+AQGALVQR+E GGPAEK GLEAGDIILK+NG AIERPSDLPRMVG+TKP Sbjct: 298 KDVAESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGSTKP 357 Query: 361 GSRATVNIWRKGSARDIAVTVVEIEADKPAKTEEKKPKQEATPNSLGLVVSDLTDAQKKE 420 G++ATV+IWRKGSARD++VTVVE+EADKP + EEKK K + PNSLGLVVSDL+DAQ+KE Sbjct: 358 GAKATVSIWRKGSARDVSVTVVELEADKPKQAEEKKAKPDNAPNSLGLVVSDLSDAQRKE 417 Query: 421 LKVDGGVLVESAVGVAAAAGLRAGDVVQRLNDVDIKDAKQFNGLVAKLDSKKRAAVLVRR 480 LKVD GVLVE+ G AAAAG+R GDV+QRLN+VD+KDAKQF LV+KLD+KKRAAVLVRR Sbjct: 418 LKVDNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKLDAKKRAAVLVRR 477 Query: 481 GDSSQFVPLRPS 492 GDSSQFVPLRP+ Sbjct: 478 GDSSQFVPLRPN 489 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 387 bits (995), Expect = e-109, Method: Compositional matrix adjust. Identities = 218/458 (47%), Positives = 292/458 (63%), Gaps = 40/458 (8%) Query: 42 DFADIVDRTGPGVVNIRTTERVKTGQPQAGADDEE--------MQEFFRRFFGVPMPRQQ 93 DF+ IV R GP VVNI T G+ + ADD++ EFF+RF P+ Q Sbjct: 64 DFSGIVQRYGPAVVNISVT-----GKARKVADDDDNGLDPNDPFSEFFKRF----GPQLQ 114 Query: 94 QPNTPNTPRPNRKGGNAQQEEVPRGVGSGFIISADGYIMTNAHVVEGASDVYVTLTDKRE 153 P QQ + G+GSGFIIS DG I+TNAHVV+GA +V V LTD+RE Sbjct: 115 MP---------------QQPRIMHGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRRE 159 Query: 154 FKAKIIGSDTRTDVALLKIDGNSLPRLVIGDSDKIRAGEWVLAIGSPFGLENTVTAGIIS 213 FKAK++G D ++D+A+++ID +LP + IGD +++ G+ VLAIGSP+G +NT TAGIIS Sbjct: 160 FKAKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIIS 219 Query: 214 AKARDTGD--YLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAVPI 271 AK+R D Y+P IQTDVAVNPGNSGGPL +L G+VIGINSQIYS++GGF G+SF++PI Sbjct: 220 AKSRSLPDDNYVPFIQTDVAVNPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPI 279 Query: 272 DEALRVSDQLKASGRVTRGRIGVQIGEVTKDVAESLGLAKAQGALVQRIEPGGPAEKGGL 331 D A++V QL G+VTRGR+GV + ++ + +++S GL KA+GALV +E G PA+K GL Sbjct: 280 DVAMKVEQQLVTHGKVTRGRLGVSVQDLNQALSDSFGLKKAEGALVSSVEKGSPADKAGL 339 Query: 332 EAGDIILKFNGTAIERPSDLPRMVGATKPGSRATVNIWRKGSARDIAVTVVEIEADKPAK 391 +AGD+I+ FNG AI+ DLP +V T PGS + + R G R I VTV E+ K AK Sbjct: 340 QAGDVIISFNGHAIDHSVDLPTLVADTPPGSTKPMEVMRAGKVRTINVTVGEM---KQAK 396 Query: 392 TEEKKPKQEATPNSLGLVVSDLTDAQKKELKVDGGVLVESAVGVAAAAGLRAGDVVQRLN 451 + K K + LGL V L ++ +L G+LVE G AA AG+++GDV+ +N Sbjct: 397 NDAKASKSDDQ-GRLGLAVRPLDKDEQAQLGGQKGLLVEDVSGPAAVAGIQSGDVILAVN 455 Query: 452 DVDIKDAKQFNGLVAKLDSKKRAAVLVRRGDSSQFVPL 489 + Q L D+ K A+LV RGD FVPL Sbjct: 456 GTPVSSVAQLRKLAT--DAGKNVALLVERGDEKIFVPL 491 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 222 bits (565), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 131/362 (36%), Positives = 200/362 (55%), Gaps = 26/362 (7%) Query: 23 PALTGLSTAVAAPAVAGLPDFADIVDRTGPGVVNIRTTERVKTGQPQAGADDEEMQEFFR 82 PA + + A P L + + P VVNI TT + PQ + FFR Sbjct: 40 PAQSTVQMQEATPNAPALDSYRHAARQAMPSVVNIFTTTEAR---PQKSPFQND--PFFR 94 Query: 83 RFFGVPMPRQQQPNTPNTPRPNRKGGNAQQEEVPRGVGSGFIISADGYIMTNAHVVEGAS 142 +FFG + QQ++ +GSG I+S GYI+TN HVVE A Sbjct: 95 KFFG------------------DQFDEQQQDDKQSSLGSGVIVSPQGYILTNNHVVEAAD 136 Query: 143 DVYVTLTDKREFKAKIIGSDTRTDVALLKIDGNSLPRLVIGDSDKIRAGEWVLAIGSPFG 202 + V L D R+ AK++G D TD+A++KID +LP + +G + G+ VLAIG+PFG Sbjct: 137 KIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPENSSVGDVVLAIGNPFG 196 Query: 203 LENTVTAGIISAKARDT---GDYLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRS 259 + TVT GI+SA R+ + IQTD A+NPGNSGG L++ G ++GIN+ IYSR+ Sbjct: 197 VGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRT 256 Query: 260 GGFMGISFAVPIDEALRVSDQLKASGRVTRGRIGVQIGEVTKDVAESLGLAKAQGALVQR 319 GG +GI FA+P+ A V + + + G+V RG IGV+ ++T ++AES GL K GA++ Sbjct: 257 GGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELAESFGLGKKTGAIIAG 316 Query: 320 IEPGGPAEKGGLEAGDIILKFNGTAIERPSDLPRMVGATKPGSRATVNIWRKGSARDIAV 379 + GGPA++ G+ GDI++ + + +++ ++ PG + + + RK + + Sbjct: 317 VLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQIAMTVLRKSQETKLNI 376 Query: 380 TV 381 TV Sbjct: 377 TV 378 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 90.5 bits (223), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 63/167 (37%), Positives = 97/167 (58%), Gaps = 9/167 (5%) Query: 120 GSGFIISADGYIMTNAHVVEGASDVYVTLTDKREFKAKIIGSDTRTDVALLKIDGNSLPR 179 G+GF I G++++N HVV A V V L RE A+++ D DVALLK + + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 180 LVIGDSDKIRAGEWVLAIGSPFG--LENTVTAGIISAKARDTGDYLPLIQTDVAVNPGNS 237 L + D+ GE V A+GSP G T+T GI+S G +Q+DVA+ PG+S Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILSGVRLLNGQE--FLQSDVAILPGSS 387 Query: 238 GGPLINLKGEVIGIN-SQIYSRSGGFMGISFAVPIDEAL-RVSDQLK 282 GGPL++ G+V+GI + + +R G G++F +P+ A+ R++ QL+ Sbjct: 388 GGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASAVERLNLQLQ 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,439,798 Number of extensions: 61935 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 4 Length of query: 496 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 407 Effective length of database: 1,198,455 Effective search space: 487771185 Effective search space used: 487771185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)