BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0092.0050.p (630 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 1140 0.0 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 163 3e-41 gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 ... 99 8e-22 gi|300313617|ref|YP_003777709.1| translation elongation factor [... 99 8e-22 gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspir... 89 8e-19 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 1140 bits (2949), Expect = 0.0, Method: Compositional matrix adjust. Identities = 550/598 (91%), Positives = 577/598 (96%), Gaps = 2/598 (0%) Query: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREMEAQVLDSMDIERERGITIKAQTAA 60 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREMEAQVLDSMDIERERGITIKAQTAA Sbjct: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREMEAQVLDSMDIERERGITIKAQTAA 60 Query: 61 LHYKARDGVIYNINLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL 120 L YKA+DG IYN+NLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL Sbjct: 61 LSYKAKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL 120 Query: 121 ELGVEVIPVLNKIDLPSADPDNAKSEIEEVIGIDASDATPCSAKTGLGVEDVIEAMIAKV 180 +LGVEV+PVLNKIDLPSADPDNAKSEIE+VIGIDASDATPCSAKTGLGVEDV+EA++AKV Sbjct: 121 DLGVEVVPVLNKIDLPSADPDNAKSEIEDVIGIDASDATPCSAKTGLGVEDVLEAIVAKV 180 Query: 181 PPPKGDPTAPLQALIVDSWFDNYVGVVMLVRVVNGTLRPKDKIFLMATESTHLTESIGVF 240 P PKGDP APLQALI+DSWFDNYVGVVMLVR+VNGTLRPKDKI LMATE+ +LTESIGVF Sbjct: 181 PAPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKILLMATETQYLTESIGVF 240 Query: 241 TPKSQVRETLSAGQVGFIIAGIKELKAAKVGDTVTLAANKAAEPLPGFKEVQPQVFAGLF 300 TPKSQ RE L+AGQVGF+IAGIKELK+A+VGDT+TLA+ A PLPGFKEVQPQVFAGLF Sbjct: 241 TPKSQSREALTAGQVGFVIAGIKELKSARVGDTITLASKPAPAPLPGFKEVQPQVFAGLF 300 Query: 301 PVEANQYDALRDSLEKLKLNDAALMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD 360 PVEANQYDALRDSLEKLKLNDAALMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD Sbjct: 301 PVEANQYDALRDSLEKLKLNDAALMYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD 360 Query: 361 MDLITTAPTVVYEVVLRDGEGTILMVDNPSKMPEPSRIEEVREPIVTVNLYMPQEYVGSV 420 MDLITTAPTVVYEV RD GT+LMVDNPSKMPE S+IEE+REPIVTVNLYMPQEYVGSV Sbjct: 361 MDLITTAPTVVYEVQQRD--GTLLMVDNPSKMPEVSKIEEIREPIVTVNLYMPQEYVGSV 418 Query: 421 ITLCTQKRGVQINMSYHGKQVQLTYEMPMAEIVLDFFDRLKSTSRGYASMDYEFKEYRSA 480 ITLCTQKRG+Q+NMSYHGKQVQLTYEMPMAEIVLDFFDRLKSTSRGYASMDYEFKEYR+A Sbjct: 419 ITLCTQKRGIQMNMSYHGKQVQLTYEMPMAEIVLDFFDRLKSTSRGYASMDYEFKEYRAA 478 Query: 481 DVVKVDMLINSEKVDALAIIVHRANSQFRGRAVAAKMRELIPRQMFDVAIQAAIGATIIS 540 DVVKVDMLINSEKVDALAIIVHRA +Q RGR VAAKMRELIPRQMFDVAIQAAIGATIIS Sbjct: 479 DVVKVDMLINSEKVDALAIIVHRAAAQIRGRQVAAKMRELIPRQMFDVAIQAAIGATIIS 538 Query: 541 RENVKALRKNVLAKCYGGDISRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQVXE 598 RENVKA+RKNVLAKCYGGDISRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQV + Sbjct: 539 RENVKAMRKNVLAKCYGGDISRKRKLLEKQKAGKKRMKQVGSVEIPQEAFLAILQVED 596 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 149/509 (29%), Positives = 238/509 (46%), Gaps = 70/509 (13%) Query: 12 HIDHGKSTLADRIIQLCGGL-SNREMEAQVLDSMDIERERGITIKAQTAALHYKARDGVI 70 H+DHGK+TL D++++ G N++++ +V+DS DIE+ERGITI ++ A+ YK Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVEYKG----- 84 Query: 71 YNINLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTALELGVEVIPVL 130 +IN++DTPGH DF EV R LS + LL+VDA +G QT AL LG++ I VL Sbjct: 85 THINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVVL 144 Query: 131 NKIDLPSADPD---NAKSEIEEVIGIDASDAT-PCSAKTGL-------------GVEDVI 173 NKID P A + NA E+ + +G P +GL +E + Sbjct: 145 NKIDRPGARAEWAINATFELFDKLGATEEQLDFPVIYASGLNGYASLDPEAREGNMEPLF 204 Query: 174 EAMIAKVPPPKGDPTAPLQALIVDSWFDNYVGVVMLVRVVNGTLRPKDKIFLMATESTHL 233 +A++ VP K DP APLQ I + +YVG + + R++ G ++ + M Sbjct: 205 DAILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRVKGGQDVVWMNGPDDKP 264 Query: 234 TES--IGVFTPKSQVRETLSAGQVG--FIIAGIKELKAAKVGDTVTLAANKAAEPLPGFK 289 T++ V T K R + G +I GI+E +G T+ + A LP K Sbjct: 265 TKARINQVLTFKGLERVLVDEALAGDIVLINGIEE-----IGIGSTICSPDAPNGLPMLK 319 Query: 290 EVQPQVFAGLF----PVEANQ-----YDALRDSLEK-LKLNDAALMYEPEVSQALGFGFR 339 +P + P+ + +RD LEK LK N A + + E + + Sbjct: 320 VDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNMALRVVQAENDDST---YE 376 Query: 340 CGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVLRDGEGTILMVDNPSKMPEPSRIE 399 G LH+ I+ E + RE +L + P VV+ +V + + Sbjct: 377 VSGRGELHLTILIENMRRE-GFELAVSRPRVVFRMV--------------------NGVR 415 Query: 400 EVREPIVTVNLYMPQEYVGSVITLCTQKRGVQINMSYHGK-QVQLTYEMPMAEIVLDFFD 458 E EP + + + + + G V+ ++RG NM GK +V+L Y +P A ++ F Sbjct: 416 E--EPYENLTVDVEETHQGGVMEELGRRRGDLQNMEPDGKGRVRLEYHIP-ARGLIGFQG 472 Query: 459 RLKSTSRGYASMDYEFKEYRSADVVKVDM 487 + +RG M + F +Y+ D K ++ Sbjct: 473 EFMTLTRGTGLMSHVFDDYKPVDTTKGEL 501 >gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 [Herbaspirillum seropedicae SmR1] Length = 530 Score = 99.4 bits (246), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 60/171 (35%), Positives = 96/171 (56%), Gaps = 15/171 (8%) Query: 5 RNFSIIAHIDHGKSTLADRI------IQLCGGLSNREMEAQVL-DSMDIERERGITIKAQ 57 R F II+H D GK+TL +++ IQL G + R+ D M+IE++RGI++ Sbjct: 10 RTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGISVA-- 67 Query: 58 TAALHYKARDGVIYNINLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCY 117 ++ + ++ RD V+ NL+DTPGH DFS + R L+A + AL+V+DA++GVE QT+ Sbjct: 68 SSVMQFEYRDHVV---NLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQTIKLLN 124 Query: 118 TALELGVEVIPVLNKIDLPSADPDNAKSEIEEVIGIDASDATPCSAKTGLG 168 +I +NK+D + D E+E V+ I + P + G+G Sbjct: 125 VCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCA---PVTWPIGMG 172 >gi|300313617|ref|YP_003777709.1| translation elongation factor [Herbaspirillum seropedicae SmR1] Length = 701 Score = 99.4 bits (246), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 59/154 (38%), Positives = 84/154 (54%), Gaps = 5/154 (3%) Query: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCG---GLSNREMEAQVLDSMDIERERGITIKAQ 57 + N RN I AHID GK+T +RI+ G + A +D M+ E+ERGITI + Sbjct: 7 IENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERGITITSA 66 Query: 58 TAALHYKARDGVI--YNINLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVAN 115 +K G + IN+IDTPGHVDF+ EV RS+ +GA++V D+ GV+ Q+ Sbjct: 67 ATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQPQSETV 126 Query: 116 CYTALELGVEVIPVLNKIDLPSADPDNAKSEIEE 149 A + V I +NK+D AD + +IEE Sbjct: 127 WRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEE 160 >gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspirillum seropedicae SmR1] Length = 701 Score = 89.4 bits (220), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 53/141 (37%), Positives = 82/141 (58%), Gaps = 7/141 (4%) Query: 5 RNFSIIAHIDHGKSTLADRIIQLCGGLSNREME----AQVLDSMDIERERGITIKAQTAA 60 RN I AHID GK+T +R++ G++++ E A +D M+ E+ERGITI + Sbjct: 11 RNIGISAHIDAGKTTTTERVL-FYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATT 69 Query: 61 LHYK--ARDGVIYNINLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYT 118 ++K A + ++IN+IDTPGHVDF+ EV RS+ +GA +V A GV+ Q+ Sbjct: 70 CYWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQ 129 Query: 119 ALELGVEVIPVLNKIDLPSAD 139 A + V + +NK+D A+ Sbjct: 130 ANKYKVPRLAFVNKMDRTGAN 150 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,670,860 Number of extensions: 65020 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 8 Length of query: 630 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 539 Effective length of database: 1,188,985 Effective search space: 640862915 Effective search space used: 640862915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)