BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0092.0029.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311287|ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine ... 230 6e-62 gi|300312880|ref|YP_003776972.1| 6,7-dimethyl-8-ribityllumazine ... 60 8e-11 >gi|300311287|ref|YP_003775379.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] Length = 169 Score = 230 bits (586), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 122/167 (73%), Positives = 144/167 (86%), Gaps = 2/167 (1%) Query: 3 HPSSNTSPAKKRIAFIQASWHKEIVDQCRISFTAEIAKLGFAESGIDFFELPGAFEIPLH 62 H S+ +S +RIAFIQA WHK+IVDQCRI+FT E+AKLG+ + IDFFELPGAFEIPLH Sbjct: 5 HLSAVSSHHGQRIAFIQACWHKDIVDQCRIAFTEEMAKLGWPAASIDFFELPGAFEIPLH 64 Query: 63 AKLLAKTGKYVGIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLDTEVPVFSAVLTPHHFH 122 AK+LAKTG+Y GIVA+GLVVDGGIYRHDFVAQ+VI++LM VQLDTEVPVFSAVLTPHHFH Sbjct: 65 AKVLAKTGRYAGIVASGLVVDGGIYRHDFVAQSVISALMQVQLDTEVPVFSAVLTPHHFH 124 Query: 123 SHGEHQQFFHEHFVVKGQEVARACADTVTKLARIEAGQGSVAVLNAA 169 SH EH +FH+HF+VKG+E A+ACA TV KLA++ A G+ A LNAA Sbjct: 125 SHSEHHDYFHQHFLVKGREAAQACATTVAKLAQVRA--GAQASLNAA 169 >gi|300312880|ref|YP_003776972.1| 6,7-dimethyl-8-ribityllumazine synthase [Herbaspirillum seropedicae SmR1] Length = 161 Score = 60.5 bits (145), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 33/107 (30%), Positives = 58/107 (54%) Query: 14 RIAFIQASWHKEIVDQCRISFTAEIAKLGFAESGIDFFELPGAFEIPLHAKLLAKTGKYV 73 RI +QA +++++ + AE+ LG A+ I +PGA EIPL + +A+T ++ Sbjct: 16 RIGIVQARFNEDVCHGLLAACLAELKHLGVADEDILHVTVPGALEIPLALQKMAETEQFD 75 Query: 74 GIVAAGLVVDGGIYRHDFVAQAVITSLMNVQLDTEVPVFSAVLTPHH 120 ++A G V+ G Y + V+ + + LD VP+ +AVLT + Sbjct: 76 ALIALGAVIRGETYHFELVSNESGAGITRIGLDFGVPIANAVLTTEN 122 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 441,707 Number of extensions: 15283 Number of successful extensions: 49 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 49 Number of HSP's successfully gapped: 2 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)