BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0092.0010.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314051|ref|YP_003778143.1| transcription regulator protein... 177 1e-45 gi|300313122|ref|YP_003777214.1| transcription regulator protein... 162 2e-41 gi|300309845|ref|YP_003773937.1| LysR family transcription regul... 157 8e-40 gi|300312645|ref|YP_003776737.1| LysR family transcription regul... 149 4e-37 gi|300310443|ref|YP_003774535.1| LysR family transcription regul... 145 3e-36 >gi|300314051|ref|YP_003778143.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 177 bits (448), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 116/295 (39%), Positives = 166/295 (56%), Gaps = 7/295 (2%) Query: 1 MKNLPPTATLRAFEVATRHATFTSASEELHVTQSAVSHQLKSLEETWGLQLFKR-GKTLS 59 ++ LP A LRAF+ A R+ F+ A+EE+HVT A+SHQ+++LEE GLQLF R GK +S Sbjct: 4 LRKLPNLAALRAFDAAARYECFSRAAEEVHVTPGAISHQVRALEEDLGLQLFLRHGKRIS 63 Query: 60 LTPAGAALAPIVREFFMNLEATLADLRVQEGKVRLKVSTTYSFALKWLLPRLPGLSQQHP 119 +T G A +R+ + LR Q + RL +STT SFA +WL PRL HP Sbjct: 64 ITETGQRFAATIRKSLSEIALAAEHLRSQNRQQRLVISTTPSFASRWLAPRLWRFIDAHP 123 Query: 120 ELLVSLETTDTAINFRPTEPDVAIRFGTGNYPGLYSEFLFREQIFPVASPDLL-QRLGTP 178 EL V L++ + R DV +RFG GNY GL SE L + +PVA+P +R Sbjct: 124 ELEVVLQSAAHLNDLRLDGIDVGLRFGRGNYSGLVSEKLSDDYYYPVAAPGYRGKRSKKL 183 Query: 179 RKPADLLRYPLLMRAGADLVPKWEHWFQQVGVSMSTLKENVRFADTNMTIEAALLGQGVA 238 +PADL LL D W+ W + G+ + + D+++T+ A G+GVA Sbjct: 184 ARPADLAHCSLLR---VDSRESWQPWLAEAGLELPEPTGGLLVEDSSLTLRQAAEGKGVA 240 Query: 239 LARSGHVEHELADGSLIRLFKLPFPSPMAYYFV-CPKGVESQPHVVSFRDWLLAE 292 LAR E++ G L+RLF + PS A+YFV P+ +E +P V +F++W+ E Sbjct: 241 LARHVIASQEISTGELVRLFDVVVPSEDAHYFVHLPEALE-KPGVAAFKEWIFEE 294 >gi|300313122|ref|YP_003777214.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 296 Score = 162 bits (411), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 105/290 (36%), Positives = 151/290 (52%), Gaps = 7/290 (2%) Query: 3 NLPPTATLRAFEVATRHATFTSASEELHVTQSAVSHQLKSLEETWGLQLFKR-GKTLSLT 61 LPP L AFE A RH +F A+EEL +T SA+SH+++ LEE G +LF R G+++ L+ Sbjct: 4 RLPPVHALSAFEAAARHNSFAIAAEELCITPSALSHRIRLLEEFVGERLFYRDGRSIGLS 63 Query: 62 PAGAALAPIVREFFMNLEATLADLRVQEGKVRLKVSTTYSFALKWLLPRLPGLSQQHPEL 121 G +VR L R + ++KV +FA L+PR+ +Q+HPE+ Sbjct: 64 EFGRRYLDVVRSALRTLTDFPMPHRNAAVQPKIKVMLPPTFARHLLMPRIADFTQRHPEI 123 Query: 122 LVSLETTDTAINFRPTEPDVAIRFGTGNYPGLYSEFLFREQIFPVASPDLLQRLGTPRKP 181 +V L + + +E DV IRFG GNYP + +E LF E F VASP L+ L + P Sbjct: 124 VVELYLSVPLYDLSLSESDVEIRFGAGNYPNIITEKLFEEPAFAVASPAYLKTLPPMKSP 183 Query: 182 ADLLRYPLLMRAGADLVPKWEHWFQQVGV-SMSTLKENVRFADTNMTIEAALLGQGVALA 240 ADL + LL A + W+ WF+ G+ +R D + +EA G GV L Sbjct: 184 ADLKQATLLRSA----LEPWQPWFEAAGLKDWVEPSAGLRVDDLGLLLEAVRHGYGVGLT 239 Query: 241 RSGHVEHELADGSLIRLFKLPFPS-PMAYYFVCPKGVESQPHVVSFRDWL 289 R E LA G ++ LF + + P AYY V + V +P V +F W+ Sbjct: 240 RQHFAEELLARGEIVELFDIRLRTPPHAYYVVYERAVRERPEVDTFISWM 289 >gi|300309845|ref|YP_003773937.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 157 bits (398), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 105/298 (35%), Positives = 158/298 (53%), Gaps = 10/298 (3%) Query: 4 LPPTATLRAFEVATRHATFTSASEELHVTQSAVSHQLKSLEETWGLQLFKRG-KTLSLTP 62 +PP +L AFE R +F A+ ELH+T SA+SHQ+ LE+ GL+LF+R K + LTP Sbjct: 2 IPPLTSLMAFEAIARRKSFNLAAAELHLTPSAISHQVARLEKLLGLRLFERSTKGVELTP 61 Query: 63 AGAALAPIVREFFMNLEATLADLRVQEGKVRLKVSTTYSFALKWLLPRLPGLSQQHPELL 122 AG + V + + DLR + L++ ++ SFA WL+PR+ +Q+HP++ Sbjct: 62 AGQSYLNRVAGALGAINSATDDLR-HGVENSLQIHSSPSFASLWLMPRIGRFTQRHPDIA 120 Query: 123 VSLETTDTAINFRPTEPDVAIRFGTGNYPGLYSEFLFREQIFPVASPDLL--QRLGTPRK 180 + L + +F + D+ IR G ++P L E + E++ P+ASPD L R+ TP Sbjct: 121 LGLSASPEISDFEIGQVDLDIRCGVPHWPDLEIEAVLSERVIPMASPDFLARHRIDTPD- 179 Query: 181 PADLLRYPLLMRAGADLVPKWEHWFQQVGVSMSTLKENVRFADTNMTIEAALLGQGVALA 240 DL+ PL+ G+ L +W WF + + + +RF M+IEAA+ G GV Sbjct: 180 --DLVLAPLIQSRGSLL--QWPEWFARYAPDLRPERIALRFDRAMMSIEAAVQGLGVVFE 235 Query: 241 RSGHVEHELADGSLIRLFKLPFPSPMA-YYFVCPKGVESQPHVVSFRDWLLAESGAAL 297 LA G L +F + +Y VCP +S+P V+ F WL ESGAAL Sbjct: 236 SDAFGAVALASGRLQPVFADGLALEITQHYAVCPARNKSRPEVMLFLQWLREESGAAL 293 >gi|300312645|ref|YP_003776737.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 287 Score = 149 bits (375), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 110/296 (37%), Positives = 153/296 (51%), Gaps = 19/296 (6%) Query: 6 PTATLRAFEVATRHATFTSASEELHVTQSAVSHQLKSLEETWGLQLFKR-GKTLSLTPAG 64 P LRAFEV+ RH +FT A+EEL VTQ+AVS Q+K+LE+ G+ LF+R + L+LT G Sbjct: 4 PLNALRAFEVSARHLSFTRAAEELSVTQTAVSMQVKNLEQRLGVALFRRLPRGLALTDEG 63 Query: 65 AALAPIVREFFMNLEATLADLRVQEGKVR--LKVSTTYSFALKWLLPRLPGLSQQHPELL 122 AL P+V + F + LA ++G+ R L V +FA+ WL+PRL QQHP + Sbjct: 64 LALLPVVAQSFGRIADVLAQF--EDGRRREVLTVGVVGTFAVGWLMPRLRQFQQQHPFVD 121 Query: 123 VSLETTDTAINFRPTEPDVAIRFGTGNYPGLYSEFLFREQIFPVASPDLLQRLGTPRKPA 182 + L T + ++ D AIRFG G + G +E LF + P+ +P + RL R PA Sbjct: 122 LRLLTNNNRVDLAGEGLDYAIRFGDGAWHGTQAERLFEAPLAPMCAPAIAARL---RSPA 178 Query: 183 DLLRYPLLMRAGADLVPKWEHWFQQVGVSMSTLKENVRFADTNMTIEAALLGQGVALARS 242 DL LL +D +W WF G + L+ V F + EAA GVAL Sbjct: 179 DLGAETLLRSYRSD---EWGRWFAAAGAACPPLRGFV-FDSSLALAEAAAQEAGVALLPV 234 Query: 243 GHVEHELADGSLIRLFKLPFPSPM---AYYFVCPKGVESQPHVVSFRDWLLAESGA 295 E EL G L+ PF + AY+ K + +FR WL + +GA Sbjct: 235 LMFERELQQGRLV----CPFGQRIVLGAYWLTSLKSRPQTAAMAAFRQWLQSHAGA 286 >gi|300310443|ref|YP_003774535.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 145 bits (367), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 101/291 (34%), Positives = 150/291 (51%), Gaps = 6/291 (2%) Query: 1 MKNLPPTATLRAFEVATRHATFTSASEELHVTQSAVSHQLKSLEETWGLQLFKR-GKTLS 59 M LPP L+AFE R +F A+ ELH+T SAVSHQ+ LE G++LF+R + Sbjct: 1 MAVLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVR 60 Query: 60 LTPAGAALAPIVREFFMNLEATLADLRVQEGKVRLKVSTTYSFALKWLLPRLPGLSQQHP 119 +T AG A ++ + A D+R Q L + + S A WL+ RL ++ +P Sbjct: 61 ITTAGEAYLARIQGAISAITAASMDVR-QGVSNTLYIHSAPSIASLWLMARLNDFARSYP 119 Query: 120 ELLVSLETTDTAINFRPTEPDVAIRFGTGNYPGLYSEFLFREQIFPVASPDLLQRLGTPR 179 E+ +L + NF E D+ IR+G N+PGL E LF E+I P+ASP ++ R Sbjct: 120 EITFNLSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRL-R 178 Query: 180 KPADLLRYPLLMRAGADLVPKWEHWFQQVGVSMSTLKENVRFADTNMTIEAALLGQGVAL 239 KPADLL L+ R+ +V +W WF + + + VRF M+++AA G GVAL Sbjct: 179 KPADLLGVKLI-RSNVSVV-QWADWFGEFADVRAPEQFAVRFDRAQMSLDAATQGLGVAL 236 Query: 240 ARSGHVEHELADGSLIRLFKLPFPSPM-AYYFVCPKGVESQPHVVSFRDWL 289 + L + L +F L + A++ V P +P V +F +WL Sbjct: 237 ESATVAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWL 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 966,036 Number of extensions: 41114 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 43 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)