BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0092.0008.p (253 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 314 5e-87 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 137 1e-33 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 135 2e-33 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 130 1e-31 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 127 1e-30 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 314 bits (804), Expect = 5e-87, Method: Compositional matrix adjust. Identities = 150/246 (60%), Positives = 192/246 (78%) Query: 8 PVILITGGSRGMGAATARLAAAQGYDVAISYVQDESAALAVVSDVEAAGRRALPVRADSA 67 VIL+TGGSRG+GAATA LA +GY VA++YV + +AA AVV+ ++AAG RA+ V D A Sbjct: 6 KVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDVA 65 Query: 68 DPEQVARLFAGIDREFGRIDVLVNNAAILQRQSRLEDIGFERMQRIFAVNAIGPMLCAQQ 127 E V +LFA +D E GR+ LVNNA +L QSRLED+ ER+ R+ N G +LCA++ Sbjct: 66 REEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCARE 125 Query: 128 AIRRMSYRHQGRGGAVINISSASARLGSPNEYVDYAASKGALETFTTGLAREVAREGIRV 187 A++RMS RH G+GGA++N+SS +A+LG P EYVDYAASKGA++ FT GLA+EVA EGIRV Sbjct: 126 AVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRV 185 Query: 188 NCIRPGHIYTDMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAVLWLASAEASFTTGTFL 247 N +RPG IYTD+HASGGE GRVDR+KD++PM RGG EVA A+LWL S EAS+ TGTF+ Sbjct: 186 NAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTFI 245 Query: 248 DVTGGK 253 DV+GG+ Sbjct: 246 DVSGGR 251 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 100/248 (40%), Positives = 137/248 (55%), Gaps = 16/248 (6%) Query: 9 VILITGGSRGMGAATARLAAAQGYDVAISYVQDESAALAVVSDVEAAGRRALPVRADSAD 68 V + GGSRG+GAA + AA+G VA +YV A A+VS V+A G RAL ++ADSAD Sbjct: 19 VAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQADSAD 78 Query: 69 P---EQVARLFAGIDREFGRIDVLVNNAAILQRQSRLEDIGFERMQRIFAVNAIGPMLCA 125 +Q RL A FGR+D+LVNNA +L L+ E + R AVN + Sbjct: 79 AAALQQAIRLAA---ETFGRLDILVNNAGVLA-MGPLDQFKLEDLDRTLAVNVRSVFVAT 134 Query: 126 QQAIRRMSYRHQGRGGAVINISSASA-RLGSPNEYVDYAASKGALETFTTGLAREVAREG 184 Q+A R M G GG VI+I S +A R+ V YA SK AL T GLAR++ G Sbjct: 135 QEAARYM-----GEGGRVISIGSTNADRIPFAGGAV-YAMSKSALVGLTKGLARDLGPRG 188 Query: 185 IRVNCIRPGHIYTDMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAVLWLASAEASFTTG 244 I VN ++PG + TDM+ + G+ + + + R G+ EE+A V +LA E+ + TG Sbjct: 189 ITVNNVQPGPVDTDMNPASGD--FAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITG 246 Query: 245 TFLDVTGG 252 L + GG Sbjct: 247 ANLMIDGG 254 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 135 bits (341), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 87/245 (35%), Positives = 129/245 (52%), Gaps = 6/245 (2%) Query: 9 VILITGGSRGMGAATARLAAAQGYDVAISYVQDESAALAVVSDVEAAGRRALPVRADSAD 68 V LITGG G+G A A A +G +AI Y+ +E A DV+A L + D Sbjct: 127 VALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGS 186 Query: 69 PEQVARLFAGIDREFGRIDVLVNNAAILQRQSRLEDIGFERMQRIFAVNAIGPMLCAQQA 128 PE + +GR+DVLVNNAA +E+I +++ F N ++A Sbjct: 187 PEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEA 246 Query: 129 IRRMSYRHQGRGGAVINISSASARLGSPNEYVDYAASKGALETFTTGLAREVAREGIRVN 188 + ++ RG +IN +S +A GS VDYAA+KGA+ FT L++++A GI VN Sbjct: 247 MPFLT-----RGARIINTTSVTAYRGS-KHLVDYAATKGAIVAFTRSLSQQLASAGIHVN 300 Query: 189 CIRPGHIYTDMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAVLWLASAEASFTTGTFLD 248 + PG I+T + + V ++P+GR GQP+EVA A ++LA+ AS+ TG L Sbjct: 301 AVAPGPIWTPLIPASFSAEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLH 360 Query: 249 VTGGK 253 GG+ Sbjct: 361 PNGGE 365 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 130 bits (326), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 90/249 (36%), Positives = 137/249 (55%), Gaps = 8/249 (3%) Query: 5 SPAPVILITGGSRGMGAATARLAAAQGYDVAISYVQDESAALAVVSDVEAAGRRALPVRA 64 S A V L+TGG+ G+GA+ ++ AA+G+ V ++ + ++A+ + AAG RA P++ Sbjct: 6 SGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAAS-KTADALVAAGHRAAPLQM 64 Query: 65 DSADPEQVARLFAGIDREFGRIDVLVNNAAILQRQSRLEDIGFERMQRIFAVNAIGPMLC 124 D PE +A FA I++++GR DVLVNNA + + S L D E + VN G +L Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFL-DYPLENWLQTMNVNVTGVLLA 123 Query: 125 AQQAIRRMSYRHQGRGGAVINISSASA-RLGSPNEYVDYAASKGALETFTTGLAREVARE 183 Q A R M + GR ++NISS S R G+ Y SK A+ T +A E+A+ Sbjct: 124 GQHAARLMVKQQWGR---IVNISSISGIRAGAGR--TAYGTSKAAVIGLTRQMAIELAQY 178 Query: 184 GIRVNCIRPGHIYTDMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAVLWLASAEASFTT 243 GI VN + PG + T M + + +PM R G +E+A AV +LA+ E+++ T Sbjct: 179 GITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYIT 238 Query: 244 GTFLDVTGG 252 G + V GG Sbjct: 239 GHVIPVDGG 247 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 127 bits (318), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 90/247 (36%), Positives = 136/247 (55%), Gaps = 13/247 (5%) Query: 9 VILITGGSRGMGAATARLAAAQGYDVAISYVQDESAALAVVSDVEAAGRRALPVRADSAD 68 V+++TGGS G+G ATA+L AA+G V +S + + AV G A+ V D+A Sbjct: 11 VVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAV----REIGNGAVGVPGDAAK 66 Query: 69 PEQVARLFAGIDREFGRIDVLVNNAAILQRQSR-LEDIGFERMQRIFAVNAIGPMLCAQQ 127 + + RL+A + + GR+DVL NA +++ L IG E +F +N G + Q+ Sbjct: 67 LDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSVQK 126 Query: 128 AIRRMSYRHQGRGGAVINISSASARLGSPNEYVDYAASKGALETFTTGLAREVAREGIRV 187 A+ MS +GGA++ S + G P + + Y ASK A+ +F ++ GIRV Sbjct: 127 ALPLMS-----QGGAIVLNGSVAGVKGFPGQSL-YNASKAAVRSFARSWTADLKERGIRV 180 Query: 188 NCIRPGHIYTDMHASGGE--PGRVDRVKDSIPMGRGGQPEEVARAVLWLASAEASFTTGT 245 N + PG T + E PG D +K +P+GR G+P+E+ RAVL+LASAE+S+ G Sbjct: 181 NVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGV 240 Query: 246 FLDVTGG 252 L V GG Sbjct: 241 ELFVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 815,507 Number of extensions: 33778 Number of successful extensions: 312 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 48 Length of query: 253 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 170 Effective length of database: 1,226,865 Effective search space: 208567050 Effective search space used: 208567050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)