BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0091.0001.p (572 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 697 0.0 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 132 5e-32 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 119 6e-28 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 114 3e-26 gi|300313022|ref|YP_003777114.1| two component sensor histidine ... 110 3e-25 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 697 bits (1800), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/392 (85%), Positives = 360/392 (91%) Query: 181 ILVIEDDTAFALILRDLAHEMGFQCIITHSANEGVAAADLYHPSAILLDMNLPDFSGLGV 240 ILVIEDD AFA+ILRDLA EMGFQC++THSANEGVAAA++Y PSAIL+DMNLPDFSGLGV Sbjct: 769 ILVIEDDAAFAMILRDLAREMGFQCVVTHSANEGVAAAEMYRPSAILMDMNLPDFSGLGV 828 Query: 241 LDQIKRNPHTRHIPVHVVSVADYIQEAMERGAVGYALKPVQREQLIDAFRKLEAKFSQSL 300 LDQIKRNP TRHIPVHVVSVADY EAMERGA+GYALKPVQRE+LI A RKLEAKF Q L Sbjct: 829 LDQIKRNPKTRHIPVHVVSVADYSHEAMERGAIGYALKPVQREELIGAIRKLEAKFLQGL 888 Query: 301 HHVLVVEDDARQRDSIRQLLGNDDVNIVGVETAAKALEQLRTTTFDCMVMDLNLPDLSGY 360 HVLVVEDDARQ DSIRQLL +++N+VGV TAA AL +LR TFDCMVMDLNLPDLSGY Sbjct: 889 RHVLVVEDDARQLDSIRQLLAGENINLVGVRTAADALARLRQNTFDCMVMDLNLPDLSGY 948 Query: 361 ELLEKMAQQEDVAFPPVIVYTGRSLTGEEEQGLRRFSRSIIIKDARSPERLLDEVTLFLH 420 +LLEKMA+QE VAFPPVIVYTGRSL+ EEEQ LRRFSRSIIIKDARSPERLLDEVTLFLH Sbjct: 949 QLLEKMAEQEHVAFPPVIVYTGRSLSAEEEQNLRRFSRSIIIKDARSPERLLDEVTLFLH 1008 Query: 421 QVETTLPAESQRLLKVARDREAVFQGRRVLVVEDDVRNIFALSSVLEPKGMKVEIARNGI 480 QVETTLPAESQR+LKVARDRE VF+GRR+LVVEDDVRNIFALSSVLEPKGMKVEIARNG Sbjct: 1009 QVETTLPAESQRMLKVARDRETVFEGRRILVVEDDVRNIFALSSVLEPKGMKVEIARNGR 1068 Query: 481 EALEALKRSQASGAQRIDLVLMDIMMPEMDGMTAMREIRRQPEWKKLPIIALTAKAMKDD 540 EAL+AL+RSQ IDLVLMDIMMPEMDG+TAMREIR+Q WK+LPIIALTAKAMKDD Sbjct: 1069 EALQALQRSQDKPDAPIDLVLMDIMMPEMDGLTAMREIRKQAVWKRLPIIALTAKAMKDD 1128 Query: 541 QEKCLAAGANDYIAKPLDVEKLLSLVRVWMPK 572 QEKCLAAGANDYIAKPLDVEKLLSLVRVWMPK Sbjct: 1129 QEKCLAAGANDYIAKPLDVEKLLSLVRVWMPK 1160 Score = 322 bits (826), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 222/567 (39%), Positives = 305/567 (53%), Gaps = 69/567 (12%) Query: 1 VEKASELERSNQYKSEFLANMSHELRTPLNSTLILAKLLADNKGGNLTDEQVRFAQTISS 60 VEKA+ELER+NQYKSEFLANMSHELRTPLNSTLILAKLLADNK GNL+DEQVRFAQTISS Sbjct: 462 VEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISS 521 Query: 61 AGNDLLALINDILDLSKMEAGKIEIVAEQVRMQKLTDELTATFQPTAQQKNLSLRTIVEA 120 AGNDLLALINDILDLSKMEAGK++IV E + KL DEL+A+F TAQQK L + VE Sbjct: 522 AGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEP 581 Query: 121 GTPEHIVTDAQRLGQILKNLLSNAVKFTDKGEVTLTIYPNAEGRVAFSVRDTGIGIADDX 180 G P+ + TD QRLGQILKNLLSNA+KFT+KGEV+L IYP+A GR+AF+VRDTGIGI +D Sbjct: 582 GVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIYPDAGGRIAFAVRDTGIGIGEDQ 641 Query: 181 ILVIED--------------DTAFAL-ILRDLAHEMGFQCIITHSANEGVAAADLYHPSA 225 I + T L I RDLAH +G +T EG Sbjct: 642 HQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGS---------- 691 Query: 226 ILLDMNLPD-FSGLGVLDQIKRNPHTRHIPVHVVSVADYIQEA----MERGAVGYALKPV 280 + ++LP +S + P A ++ A + R A + + Sbjct: 692 -IFTLSLPPLYSSEQAVSNTAPEPTPAAAAAASTLQAAHVATAPDVRINRTPAAAAPQRL 750 Query: 281 QREQLIDAFRKLEAKFSQSLHHVLVVEDDARQRDSIRQLLGNDDVNIVGVETAAKALEQL 340 Q + D ++E S +LV+EDDA +R L V +A + + Sbjct: 751 QEISIDDDRDRIET----SSRLILVIEDDAAFAMILRDLAREMGFQCVVTHSANEGVAAA 806 Query: 341 RTTTFDCMVMDLNLPDLSGYELLEKMAQQEDVAFPPVIVYTGRSLTGEEEQGLRRFSRSI 400 ++MD+NLPD SG +L+++ + PV V S+ + + R + Sbjct: 807 EMYRPSAILMDMNLPDFSGLGVLDQIKRNPKTRHIPVHVV---SVADYSHEAMERGAIGY 863 Query: 401 IIKDARSPERLLDEVTLFLHQVETTLPAESQRLLKVARDREAVF-QG-RRVLVVEDDVRN 458 +K P + + L+ R EA F QG R VLVVEDD R Sbjct: 864 ALK-----------------------PVQREELIGAIRKLEAKFLQGLRHVLVVEDDARQ 900 Query: 459 IFALSSVLEPKGMKVEIARNGIEALEALKRSQASGAQRIDLVLMDIMMPEMDGMTAMREI 518 + ++ +L + + + R +AL L+++ D ++MD+ +P++ G + ++ Sbjct: 901 LDSIRQLLAGENINLVGVRTAADALARLRQNT------FDCMVMDLNLPDLSGYQLLEKM 954 Query: 519 RRQPEWKKLPIIALTAKAMKDDQEKCL 545 Q P+I T +++ ++E+ L Sbjct: 955 AEQEHVAFPPVIVYTGRSLSAEEEQNL 981 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 132 bits (333), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 110/370 (29%), Positives = 169/370 (45%), Gaps = 72/370 (19%) Query: 8 ERSNQYKSEFLANMSHELRTPLNSTLILAKLLADNKGGNLTDEQVRFAQTISSAGNDLLA 67 E +N KS F+A MSHELRTPLNS L A++L + + + I +G LL+ Sbjct: 682 ESANLAKSRFVAGMSHELRTPLNSILGYAQIL--HVDATIPPARKDAIGVIRRSGEHLLS 739 Query: 68 LINDILDLSKMEAGKIEIVAEQVRMQKLTDELTATFQPTAQQKNLSLRTIVEAGTPEHIV 127 LI+ +LD++K+EAGK+ + ++++ ++ +++ F P A+QK L R P+ + Sbjct: 740 LIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQKGLHFRFDKSGRLPQIVH 799 Query: 128 TDAQRLGQILKNLLSNAVKFTDKGEVTLTIYPNAEGRVAFSVRDTGIGIADD----XILV 183 D +RL QIL NLL NAVKFTD G VTL + A F V DTGIGIADD L Sbjct: 800 ADQKRLRQILINLLGNAVKFTDHGGVTLRLR-YAREIAYFDVIDTGIGIADDDRERIFLP 858 Query: 184 IEDDTAFAL-----------ILRDLAHEMGFQCIITHSANEGVAAADLYHPSAILLDMNL 232 E +A L I R L H MG + + +G S+ L + L Sbjct: 859 FERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQVGQG---------SSFHLKIYL 909 Query: 233 PDFSGLGVLDQIKRNPHTRHIPVHVVSVADYIQEAMERGAVGYALKPVQREQLIDAFRKL 292 P+ H+P + + D + GYA P + Sbjct: 910 PEV----------------HVPRPRIKLEDQMS--------GYA-GPSR----------- 933 Query: 293 EAKFSQSLHHVLVVEDDARQRDSIRQLLGNDDVNIVGVETAAKALEQLRTTTFDCMVMDL 352 H+LVV+D A QR ++ +L + ++ +E L D +++D+ Sbjct: 934 ---------HILVVDDQASQRRLLKDMLMPLGFTVSEADSGLACMETLGHALPDLVLLDI 984 Query: 353 NLPDLSGYEL 362 +P + G+ + Sbjct: 985 AMPQMDGWSV 994 Score = 67.0 bits (162), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 42/121 (34%), Positives = 64/121 (52%), Gaps = 7/121 (5%) Query: 447 RRVLVVEDDVRNIFALSSVLEPKGMKVEIARNGIEALEALKRSQASGAQRIDLVLMDIMM 506 R +LVV+D L +L P G V A +G+ +E L G DLVL+DI M Sbjct: 933 RHILVVDDQASQRRLLKDMLMPLGFTVSEADSGLACMETL------GHALPDLVLLDIAM 986 Query: 507 PEMDGMTAMREIRRQPEWKKLPIIALTAKAMKDDQEKCLAAGANDYIAKPLDVEKLLSLV 566 P+MDG + R IR + +LPI+ ++A A ++ E+ ND++ KP+ +LL + Sbjct: 987 PQMDGWSVARAIRAR-GLAQLPILLVSANAFENLHERNEPPLVNDFVVKPVSYHELLGKI 1045 Query: 567 R 567 R Sbjct: 1046 R 1046 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 119 bits (298), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 67/182 (36%), Positives = 110/182 (60%), Gaps = 9/182 (4%) Query: 6 ELERSNQYKSEFLANMSHELRTPLNSTLILAKLLADNKGGNLTDEQVRFAQTISSAGNDL 65 E + +N+ KS FLA +SHE+RTP+N L L++LL ++ L+ +Q +A +I A L Sbjct: 340 EADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMES---GLSSKQRDYADSIHGATGGL 396 Query: 66 LALINDILDLSKMEAGKIEIVAEQVRMQKLTDELTATFQPTAQQKNLSLRTIVEAGTPEH 125 + +INDILD SK+E+GK+++ +L EL+ ++P+ Q+K++ ++ Sbjct: 397 VRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQFDLHLDCPENLS 456 Query: 126 IVTDAQRLGQILKNLLSNAVKFTDKGEVTLTIYPN------AEGRVAFSVRDTGIGIADD 179 +V D RL Q+L NLLSNA+KFT G V L++ + R+ F++ DTGIGI+ + Sbjct: 457 VVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFFAITDTGIGISPE 516 Query: 180 XI 181 + Sbjct: 517 AL 518 Score = 89.4 bits (220), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 53/134 (39%), Positives = 76/134 (56%), Gaps = 9/134 (6%) Query: 442 AVFQGRRVLVVEDDVRNIFALSSVLEPKGMKVEIARNGIEALEALKRSQASGAQRIDLVL 501 ++ G R+L+ ED N L +L KG ++ NG++A+EA++ DLVL Sbjct: 637 SLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTGH------YDLVL 690 Query: 502 MDIMMPEMDGMTAMREIRRQPEWKKLP---IIALTAKAMKDDQEKCLAAGANDYIAKPLD 558 MD MMP MDG A EIR + LP IIALTA A++ D+++CL AG +DY++KP Sbjct: 691 MDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDYLSKPFT 750 Query: 559 VEKLLSLVRVWMPK 572 L+ V W+ K Sbjct: 751 QVGLMQTVEKWLQK 764 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 114 bits (284), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 63/172 (36%), Positives = 100/172 (58%), Gaps = 7/172 (4%) Query: 14 KSEFLANMSHELRTPLNSTLILAKLLADNKGGNLTDEQVRFAQTISSAGNDLLALINDIL 73 + FL+ MSHE+RTP+N+ L +A LL + L +Q FA+ I + + LL ++ND+L Sbjct: 87 RGAFLSTMSHEIRTPMNAVLGMAGLLRSTR---LDTQQAEFAKAIEDSAHALLGIVNDVL 143 Query: 74 DLSKMEAGKIEIVAEQVRMQKLTDELTATFQPTAQQKNLSLRTIVEAGTPEHIVTDAQRL 133 D SK++ GK++I + + K+ + A++K + L +++ P +V D R+ Sbjct: 144 DFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKAREKRVRLLAHLDSRLPPRLVGDPGRI 203 Query: 134 GQILKNLLSNAVKFTDKGEVTLTI----YPNAEGRVAFSVRDTGIGIADDXI 181 QIL NLL NA+KFT GEV L + + RV F VRD+GIG+ +D + Sbjct: 204 RQILLNLLGNALKFTHAGEVLLRVQALQVTSDHCRVRFEVRDSGIGMDEDTL 255 Score = 89.7 bits (221), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 56/140 (40%), Positives = 76/140 (54%), Gaps = 7/140 (5%) Query: 432 RLLKVARDREAVFQGRRVLVVEDDVRNIFALSSVLEPKGMKVEIARNGIEALEALKRSQA 491 R+ A R+ R +L+VED+V N L G +IA NG+EALE L R Sbjct: 461 RMAAPATPRQGHSGQRDILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLAR--- 517 Query: 492 SGAQRIDLVLMDIMMPEMDGMTAMREIRR-QPEWKKLPIIALTAKAMKDDQEKCLAAGAN 550 R LVLMD MP +DG R IRR Q E I+A+TA AM D+E+CL AG + Sbjct: 518 ---HRYALVLMDCQMPLLDGYETTRRIRRMQGELAHTTIVAMTANAMAGDRERCLEAGMD 574 Query: 551 DYIAKPLDVEKLLSLVRVWM 570 DY+ KPL +L +++ ++ Sbjct: 575 DYLTKPLLRSQLSAMLAHYL 594 >gi|300313022|ref|YP_003777114.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 110 bits (275), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 61/177 (34%), Positives = 101/177 (57%), Gaps = 3/177 (1%) Query: 1 VEKASELERSNQYKSEFLANMSHELRTPLNSTLILAKLLADNKGGNLTDEQVRFAQTISS 60 V E ++NQ KS FL+NMSHE RTPLN L + L + G ++A I Sbjct: 318 VRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREELG---ESPLAQYAVAIHD 374 Query: 61 AGNDLLALINDILDLSKMEAGKIEIVAEQVRMQKLTDELTATFQPTAQQKNLSLRTIVEA 120 +GN LL L+N IL+++++E ++ + + +++L + A P AQ K L L + Sbjct: 375 SGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQAQAKGLPLECRIGE 434 Query: 121 GTPEHIVTDAQRLGQILKNLLSNAVKFTDKGEVTLTIYPNAEGRVAFSVRDTGIGIA 177 P+ +V D +++ ++L NL+SNAV++TD G V + + + ++ ++DTGIGIA Sbjct: 435 DVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGPQQLTMRIKDTGIGIA 491 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,588,127 Number of extensions: 64635 Number of successful extensions: 640 Number of sequences better than 1.0e-08: 57 Number of HSP's gapped: 596 Number of HSP's successfully gapped: 62 Length of query: 572 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 482 Effective length of database: 1,193,720 Effective search space: 575373040 Effective search space used: 575373040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)