BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0089.0001.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 201 3e-53 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 169 3e-43 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 157 7e-40 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 142 3e-35 gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sen... 140 1e-34 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 201 bits (512), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 98/247 (39%), Positives = 162/247 (65%), Gaps = 2/247 (0%) Query: 1 MKWFYNLKITHKLLLSFVAVLILTALLGVFSVAQIARVNQASNDISTNWLPAMRAALQMK 60 MKWFY+L+I+ KL+ +F+ V+ + A LGVFS+ Q+ +VN AS +ISTNWLP++R +++ Sbjct: 1 MKWFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQ 60 Query: 61 AALSRVRVAELQHIQSDNEEDFSRYEKSIEGQLAELKKFDSIYAPLISEPDEKIIYPQFK 120 A+SR+R ELQHI + + +++ E+ ++ Q+ LK + Y ISEP+EK IYP+ + Sbjct: 61 LAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVE 120 Query: 121 KNLAAYLAENQKIVMLARGGLSDEARLLIRGDSTRIYLAMTEQLDRLSGINEAGSAVADQ 180 K +A +++E+ KI+ L+R G +EAR L RG+ST+ Y +Q+D+L+ +N+ G+ ++++ Sbjct: 121 KLIATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISNK 180 Query: 181 TATSTYEAARIWIVVILGISVALGLLLAFWVAAMVXLGLLLAFWVAARRAANE--GRTEP 238 A + Y AR+WI+V + + L +LLA WV+ ++ L A VA R A + R EP Sbjct: 181 QADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEP 240 Query: 239 RPRRRTA 245 + T Sbjct: 241 AGKDETG 247 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 169 bits (427), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 90/230 (39%), Positives = 143/230 (62%) Query: 1 MKWFYNLKITHKLLLSFVAVLILTALLGVFSVAQIARVNQASNDISTNWLPAMRAALQMK 60 M WF NLKI KL++SF AV++LT +LG+F++ ++ VNQAS D++TNWLP ++AA ++ Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 Query: 61 AALSRVRVAELQHIQSDNEEDFSRYEKSIEGQLAELKKFDSIYAPLISEPDEKIIYPQFK 120 A+L R+R++ELQ + + + K+I+ +L L K + Y ISE +EK++Y +F Sbjct: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 Query: 121 KNLAAYLAENQKIVMLARGGLSDEARLLIRGDSTRIYLAMTEQLDRLSGINEAGSAVADQ 180 K+ A YL ++++ ++ G +EAR L +GDS + + +M E +D + +N+AGS +D Sbjct: 121 KSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 Query: 181 TATSTYEAARIWIVVILGISVALGLLLAFWVAAMVXLGLLLAFWVAARRA 230 A Y +A +WI+ +L V + L LA VA V L A VA R A Sbjct: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVA 230 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 157 bits (398), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 93/248 (37%), Positives = 144/248 (58%), Gaps = 2/248 (0%) Query: 1 MKWFYNLKITHKLLLSFVAVLILTALLGVFSVAQIARVNQASNDISTNWLPAMRAALQMK 60 M WFYNL I KL LSF V+ LT L G+F++ ++ +VN+AS +I + W+P M AA +++ Sbjct: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 Query: 61 AALSRVRVAELQHIQSDNEEDFSRYEKSIEGQLAELKKFDSIYAPLISEPDEKIIYPQFK 120 +L +R+ EL+ + + ++ + K+I+ +L L + +I+ +SEP EK Y QF Sbjct: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 Query: 121 KNLAAYLAENQKIVMLARGGLSDEARLLIRGDSTRIYLAMTEQLDRLSGINEAGSAVADQ 180 KNL AY+ ++++ +A DE R L GDS ++Y +M LD + IN +GSA AD+ Sbjct: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSGSAQADE 180 Query: 181 TATSTYEAARIWIVVILGISVALGLLLAFWVAAMVXLGLLLAFWVAARRAANE--GRTEP 238 A++ + A+ WIV +L +V + LLAF VA V L A +A R A + R P Sbjct: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 Query: 239 RPRRRTAA 246 R T A Sbjct: 241 AGRDETGA 248 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 142 bits (358), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 83/234 (35%), Positives = 137/234 (58%), Gaps = 2/234 (0%) Query: 1 MKWFYNLKITHKLLLSFVAVLILTALLGVFSVAQIARVNQASNDISTNWLPAMRAALQMK 60 M WFYNLKI HKL +F+ VL+ LG FS ++A VN++S DI+ NWLP++ A +M Sbjct: 1 MNWFYNLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMS 60 Query: 61 AALSRVRVAELQH-IQSDNEEDFSRYEKSIEGQLAELKKFDSIYAPLISEPDEKIIYPQF 119 AL+R R ++Q + +D +E+ + E++ + Q+A L K + Y L+SEP EK +YP+ Sbjct: 61 LALARSRSFDMQQLLTADKQEEKASRERA-DKQMATLLKEVARYGKLVSEPREKELYPEI 119 Query: 120 KKNLAAYLAENQKIVMLARGGLSDEARLLIRGDSTRIYLAMTEQLDRLSGINEAGSAVAD 179 ++N+A Y ++++ L A L+ ST +Y + EQ+ LS +N G+A++D Sbjct: 120 ERNIALYKEAHERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGAALSD 179 Query: 180 QTATSTYEAARIWIVVILGISVALGLLLAFWVAAMVXLGLLLAFWVAARRAANE 233 A Y A + I+ ++ V L + L++W+A +V L A +A + AA + Sbjct: 180 ADADHIYGQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGD 233 >gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 140 bits (352), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 78/199 (39%), Positives = 120/199 (60%), Gaps = 9/199 (4%) Query: 1 MKWFYNLKITHKLLLSFVAVLILTALLGVFSVAQIARVNQASNDISTNWLPAMRAALQMK 60 M WFYN +I KLLL+F VL+LT LG+FS+ Q+ +VNQA+ +++TNWLP++R+ MK Sbjct: 1 MNWFYNRRIATKLLLAFACVLLLTGGLGIFSIVQLHKVNQAATELATNWLPSIRSLNDMK 60 Query: 61 AALSRVRVAELQHIQSDNEEDFSR-----YEKSIEGQLAELKKFDSIYAPLISEPDEKII 115 ALSRVR + Q D +E S K++E LAE +K Y ISEP EK + Sbjct: 61 VALSRVRANQAQLALYDGDEKQSSAVIGLMNKNLEA-LAEARKQ---YTGQISEPGEKAL 116 Query: 116 YPQFKKNLAAYLAENQKIVMLARGGLSDEARLLIRGDSTRIYLAMTEQLDRLSGINEAGS 175 +P + ++A + + R G DEA+ L+ G + YL++ LD+L+ +N GS Sbjct: 117 FPGVNARIDKFIALHGDTLAAVRAGKLDEAKRLLLGPAFENYLSLLNDLDKLAQVNLDGS 176 Query: 176 AVADQTATSTYEAARIWIV 194 + ++A +TY+ A++W+V Sbjct: 177 VQSTRSAETTYDQAQVWVV 195 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 647,325 Number of extensions: 22617 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 12 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)