BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0088.0033.p (200 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 132 2e-32 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 131 4e-32 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 110 7e-26 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 102 3e-23 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 101 5e-23 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 132 bits (332), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 71/186 (38%), Positives = 113/186 (60%), Gaps = 1/186 (0%) Query: 10 IFRDKRMWILTLIYMFNAVAIYGVVLWLPQIVKSLGGLNNFQTGIVSAIPFICAAVGLVL 69 +F + R+W+ LIY + +YGV WLP I+K+ G + G+++AIP+ AA +VL Sbjct: 246 MFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVL 305 Query: 70 VARSSDRTGERKMHTACAGLFGGV-FLAASALVPSPVLGLVLICIAAMGLWATLGVFWTL 128 + RS+D ER+ H A G + L ++ + L + + +A++G+ TL +FW+L Sbjct: 306 IGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSL 365 Query: 129 PTQFLTGAAAAGGIAMINGFAQIGGFIGPYLVGWIRETTHSFSTALLVLAVSPMIGFVLC 188 PT FL+G AAA GIA+IN + GF+ P+LVGW+++ T S + + VLA S +IG +L Sbjct: 366 PTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLT 425 Query: 189 ATLKIR 194 TL + Sbjct: 426 LTLPAK 431 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 131 bits (329), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 76/194 (39%), Positives = 121/194 (62%), Gaps = 3/194 (1%) Query: 2 HAQHEFREIFRDKRMWILTLIYMFNAVAIYGVVLWLPQIVKSLGGLNNFQTGIVSAIPFI 61 ++H F I +D R+W++ LIY + YG+ LW+P +VK+ G N + G++SAIPF Sbjct: 240 ESKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFG 299 Query: 62 CAAVGLVLVARSSDRTGERKMHTACAGLFGGV-FLAASALVPSPVLGLVLICIAAMGLWA 120 CA + + L+ RS+DR ER+ H L GGV F+ A+ + + + + +AA G+ Sbjct: 300 CAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLT 359 Query: 121 TLGVFWTLPTQFLTGAAAAGGIAMINGFAQIGGFIGPYLVGWIRETTHSFSTALLVLAVS 180 +FW+LPT FL+GAAAA GIA IN + GF+ PYL+G++++ TH+ +T + +LAV Sbjct: 360 CAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVM 419 Query: 181 PMIGFVLCATLKIR 194 ++G + ATL + Sbjct: 420 LVVGSI--ATLATK 431 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 110 bits (276), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 63/187 (33%), Positives = 109/187 (58%), Gaps = 1/187 (0%) Query: 5 HEFREIFRDKRMWILTLIYMFNAVAIYGVVLWLPQIVKSLGGLNNFQTGIVSAIPFICAA 64 ++ ++ F + R++ L + + LWLPQI++ G L + Q G+V+AIP++ A Sbjct: 246 NKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFG-LPSHQVGLVAAIPYLVGA 304 Query: 65 VGLVLVARSSDRTGERKMHTACAGLFGGVFLAASALVPSPVLGLVLICIAAMGLWATLGV 124 V + L AR ++R+ R A A + + L SA + +P+L ++ I +A + + Sbjct: 305 VAMTLWARLANRSERRLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQAT 364 Query: 125 FWTLPTQFLTGAAAAGGIAMINGFAQIGGFIGPYLVGWIRETTHSFSTALLVLAVSPMIG 184 FW +P+ FLTG AAAGG+A+I +GGF+GP ++G+IRE T F+ L+ +A + ++G Sbjct: 365 FWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLG 424 Query: 185 FVLCATL 191 V+ L Sbjct: 425 AVITLAL 431 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 102 bits (253), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 58/186 (31%), Positives = 93/186 (50%), Gaps = 1/186 (0%) Query: 9 EIFRDKRMWILTLIYMFNAVAIYGVVLWLPQIVKSLGGLNNFQTGIVSAIPFICAAVGLV 68 + RD ++ + IY + IYG WLP I+K +G N+FQ G+ ++IP+I A + Sbjct: 245 SLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMY 304 Query: 69 LVARSSDRTGERKMHTACAGLFGGVFLAASALVPSPVLGLVLICIAAMGLWATLGVFWTL 128 L A + R + + A + +A SA V PV+G V +C A +G A +FW + Sbjct: 305 LFAVLAARYKKPQFWVATTLVISAAGMAISA-VAGPVIGFVAVCFAGIGFKAASSLFWPI 363 Query: 129 PTQFLTGAAAAGGIAMINGFAQIGGFIGPYLVGWIRETTHSFSTALLVLAVSPMIGFVLC 188 P +L AA +A+IN +GGF P G++ + T S L L+ + ++ VL Sbjct: 364 PQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLV 423 Query: 189 ATLKIR 194 + R Sbjct: 424 FLARTR 429 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 101 bits (251), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 63/181 (34%), Positives = 103/181 (56%), Gaps = 1/181 (0%) Query: 4 QHEFREIFRDKRMWILTLIYMFNAVAIYGVVLWLPQIVKSLGGLNNFQTGIVSAIPFICA 63 F++ + R+WI +Y + W P I+K +G N Q G++SAIPFI Sbjct: 245 HFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAG 304 Query: 64 AVGLVLVARSSDRTGERKMHTACAGLFGGVFLAASAL-VPSPVLGLVLICIAAMGLWATL 122 + ++ SD+TGER++H+A A L + L A+ + + + L L + +AA+G+ A Sbjct: 305 TLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAF 364 Query: 123 GVFWTLPTQFLTGAAAAGGIAMINGFAQIGGFIGPYLVGWIRETTHSFSTALLVLAVSPM 182 VFW++P FL G AAAGGIA+IN + GF+ PY++G ++ +T S S+ L +A + Sbjct: 365 PVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALEL 424 Query: 183 I 183 + Sbjct: 425 V 425 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 594,970 Number of extensions: 22689 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 13 Length of query: 200 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 119 Effective length of database: 1,236,335 Effective search space: 147123865 Effective search space used: 147123865 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)