BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0088.0022.p (523 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 955 0.0 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 221 2e-58 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 202 7e-53 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 171 1e-43 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 154 1e-38 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 955 bits (2468), Expect = 0.0, Method: Compositional matrix adjust. Identities = 461/527 (87%), Positives = 489/527 (92%), Gaps = 5/527 (0%) Query: 2 GHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSG 61 G MLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSG Sbjct: 86 GDARMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSG 145 Query: 62 GPMEAGKVVKVVNNDRKVIKLDLIDAMIKAGDNTVSDADVAEIERSACPTCGSCSGMFTA 121 GPMEAGKV+K VN D+KVIKLDL+DAMI+AGD +SDADVAE+ERSACPTCGSCSGMFTA Sbjct: 146 GPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTA 205 Query: 122 NSMNCLAEALGLALPGNGTIVATHADRKELFLGAGRLIVELAKRHYEQDDYSVLPRSIAS 181 NSMNCL E LGL+LPGNGTIVATHADRKELFL AGRLIVELAKRHYEQDDYS+LPR+I + Sbjct: 206 NSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRNICT 265 Query: 182 KAAFENAMALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDKY 241 K +ENAM LDVSMGGSTNTVLHLLAAAHEA+VEFTMADIDRISR+VPCLCKVAPMT+KY Sbjct: 266 KETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPMTNKY 325 Query: 242 HIEDVHRAGGIVAV-----RAGLLDTSLPTVHSKTMGEAIEKYDIRVSSDAAVHKLFRAA 296 HIEDVHRAGGI+++ RAGLLDTS PTVHSKT+GEAIEK+DIRVSSD AVHKLFRAA Sbjct: 326 HIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLFRAA 385 Query: 297 PGGVPSQTAFSQAERYEHGDLDRSAGCIRDIEHAYSKDGGLAVLYGNIAEKGCIVKTAGV 356 PGGVP+Q AFSQ ER+E DLDR+ GCIRD EHAYS+DGGLAVLYGNIAEKGCIVKTAGV Sbjct: 386 PGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGV 445 Query: 357 DESILKFSGKARVFESQDAAVEGILGDTVHAGDVVIIRYEGPKGGPGMQEMLYPTSYIKS 416 DESILKFSG ARVFESQDAAVEGILGD V AGDVVI+RYEGPKGGPGMQEMLYPTSYIKS Sbjct: 446 DESILKFSGTARVFESQDAAVEGILGDKVKAGDVVIVRYEGPKGGPGMQEMLYPTSYIKS 505 Query: 417 KGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPARTINLRVA 476 KGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIP RTINLR++ Sbjct: 506 KGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPNRTINLRIS 565 Query: 477 DDQLAQRRAAMEARGRDAWQPVNRERVVSQALQAYAALTTSADRGPV 523 D LA+RR AMEARG AWQP NR+RVVSQALQAYAALTTSADRG V Sbjct: 566 SDDLAKRRTAMEARGDAAWQPGNRQRVVSQALQAYAALTTSADRGAV 612 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 221 bits (562), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 158/492 (32%), Positives = 258/492 (52%), Gaps = 65/492 (13%) Query: 6 MLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPME 65 MLY R+L + VE + A+ D +V + CDK TP +LM A +++P + VSGGPM Sbjct: 98 MLY----RNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASVDVPTIGVSGGPML 153 Query: 66 AGKVV-KVVNNDRKVIKLDLIDAMIKAGDNTVSDADVAEIERSACPTCGSCSGMFTANSM 124 +GK + + + V ++ ++AG ++ + E E + G C M TA++M Sbjct: 154 SGKYRGRELGSGTGVWQMS---EDVRAG--KMTQEEFFEAESCMHRSHGHCMTMGTASTM 208 Query: 125 NCLAEALGLALPGNGTIVATHADRKELFLGAGRLIVELAKRHYEQDDYSVLPRSIASKAA 184 + EALG+ LP N I A A R L AGR IV++ E+D ++ I ++ A Sbjct: 209 ASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV----EED---LVLSKILTRQA 261 Query: 185 FENAMALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDKYHIE 244 FENA+ ++ ++GGSTN V+HLLA A ++ + D D I +++PCL + P + K+ +E Sbjct: 262 FENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQP-SGKFLME 320 Query: 245 DVHRAGGIVAVRAGL---LDTSLPTVHSKTMGEAIEKYDIRVSSDAAVHKLFRAAPGGVP 301 D + AGG+ AV L +D + T + KT+ E + AP Sbjct: 321 DFYYAGGLPAVIRQLESVIDKTALTANGKTLWENCQD-----------------AP---- 359 Query: 302 SQTAFSQAERYEHGDLDRSAGCIRDIEHAYSKDGGLAVLYGNIAEKGCIVKTAGVDESIL 361 + + IR + + + G+A+L GN+A G ++K + ++L Sbjct: 360 ----------------NWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALL 403 Query: 362 KFSGKARVFESQDAAVEGILGDTVHAGD--VVIIRYEGPKGGPGMQE---MLYPTSYIKS 416 K G+A VFE+ D + I + + + V++++ GPKG PGM E M P ++ Sbjct: 404 KHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILR- 462 Query: 417 KGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPARTINLRVA 476 KG+ + +D R SG + G V+ H SPEAA GG + +V+ GD I++D+ AR ++L V+ Sbjct: 463 KGITDMVRV-SDARMSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVS 521 Query: 477 DDQLAQRRAAME 488 D++LA+RRA + Sbjct: 522 DEELARRRAQWQ 533 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 202 bits (513), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 160/496 (32%), Positives = 256/496 (51%), Gaps = 73/496 (14%) Query: 12 SRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVV- 70 +R+L + VE + + DA+V ++ CDK TP +LM A ++P + V+GGPM GK Sbjct: 102 TRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQG 161 Query: 71 KVVNNDRKVIKLDLIDAMIKAGDNTVSDADVAE--IERSACPTCGSCSGMFTANSMNCLA 128 + + + V +L +KAG+ T+ D AE + RSA G+C+ M TA++M C+A Sbjct: 162 RDIGSGTVVWQL---SEQVKAGEITIHDFMAAEAGMSRSA----GTCNTMGTASTMACMA 214 Query: 129 EALGLALPGNGTIVATHADRKELFLGAGRLIVELAKRHYEQDDYSVLPRSIASKAAFENA 188 E+LG++LP N I A A R L +G IV++ +E S I ++ AFENA Sbjct: 215 ESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMV---WEGLTLS----KILTRKAFENA 267 Query: 189 MALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDKYHIEDVHR 248 + + ++GGSTN V+HL A A V+ + D RI R P + + P + +Y +E+ + Sbjct: 268 IRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQP-SGRYLMEEFYY 326 Query: 249 AGGIVAV--RAGLLDTSLP-----TVHSKTMGEAIEKYDIRVSSDAAVHKLFRAAPGGVP 301 AGG+ AV R G D LP TV+ +TM + ++ D + +D V Sbjct: 327 AGGLPAVLRRLGEADL-LPHKDALTVNGQTMWDNVK--DAPIYNDEVV------------ 371 Query: 302 SQTAFSQAERYEHGDLDRSAGCIRDIEHAYSKDGGLAVLYGNIAEKGCIVKTAGVDESIL 361 R + +DGG+ +L GN+A +G ++K + ++ Sbjct: 372 -----------------------RPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELM 408 Query: 362 KFSGKARVFESQDAAVEGILGD--TVHAGDVVIIRYEGPKGGPGMQE---MLYPTSYIKS 416 K G+A VFE + E I V A V++++ GPKG PGM E M P + + Sbjct: 409 KHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLAT 468 Query: 417 KGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPARTINLRVA 476 G+ K +D R SG + G VI H +PEAA GG +G+V++GD I++D A + L ++ Sbjct: 469 -GV-KDMVRISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDIS 526 Query: 477 DDQLAQRRAAMEARGR 492 ++++ +R +EAR + Sbjct: 527 EEEMKRR---LEARAK 539 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 171 bits (434), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 152/517 (29%), Positives = 245/517 (47%), Gaps = 80/517 (15%) Query: 13 RDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVV-K 71 R+L + +++ + DA+V + CDK TP +MAA ++IP + +SGGPM G + + Sbjct: 105 RNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGE 164 Query: 72 VVNNDRKVIKLDLIDAMIKAGDNTVSDADVAEIERSACPTCGSCSGMFTANSMNCLAEAL 131 +V + + K ++ AG ++ + EI ++ P+ G C+ M TA++MN +AEAL Sbjct: 165 LVGSGSAIWKGR---KLLSAG--SIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEAL 219 Query: 132 GLALPGNGTIVATHADRKELFLGAGRLIVELAKRHYEQDDYSVLPRSIASKAAFENAMAL 191 G++L G I A + +R ++ GR IV +A YE + P +I ++ AF +A+ + Sbjct: 220 GMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YE----DLRPSAILTRDAFLDAIVV 272 Query: 192 DVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDKYHIEDVHRAGG 251 + ++GGSTN H++A A A VE D + VP L + P KY E HRAGG Sbjct: 273 NAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQP-AGKYLGERFHRAGG 331 Query: 252 IVAV-----RAGLLDTSLPTVHSKTMGEAIEKYDIRVSSDAAVHKLFRAAPGGVPSQTAF 306 + A+ +AG L T KTM E ++ R S+D + F AAP Sbjct: 332 VPAIMWELQQAGKLRAERITATGKTMAENLQG---RASNDREMIYPF-AAP--------- 378 Query: 307 SQAERYEHGDLDRSAGCIRDIEHAYSKDGGLAVLYGNIAEKGCIVKTAGVDESI----LK 362 + G VL GN+ + I+KT+ + E+ L Sbjct: 379 ------------------------LRERAGFLVLKGNLFDF-AIMKTSVISETFRERYLS 413 Query: 363 FSGKARVFESQDAAVEGILGDTVHA-----------GDVVIIRYEGPKGGPGMQEM--LY 409 G+ +FE + +G D HA ++ IR GP G PG E+ + Sbjct: 414 TPGQENIFECRAVVFDG--SDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQ 471 Query: 410 PTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPAR 469 P + +G+ L DGR SG S I +ASPE+A GG + + +GD + ID+ Sbjct: 472 PPDALIKRGVSTLPTL-GDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTG 530 Query: 470 TINLRVADDQLAQRRA---AMEARGRDAWQPVNRERV 503 N+ V++++LA+R++ + WQ + R V Sbjct: 531 ECNMLVSEEELARRKSEGIPPVPPSQTPWQEIYRSTV 567 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 154 bits (390), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 147/489 (30%), Positives = 229/489 (46%), Gaps = 41/489 (8%) Query: 2 GHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRL-NIPVVFVS 60 G GM SL SRD IA + ++ + DA + + CDKI PG+L+ A+ ++P VFV Sbjct: 120 GQAGMELSLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVP 179 Query: 61 GGPMEAGKVVKVVNNDRKVIKLDLIDAMIKAGDNTVSDADVAEIERSACPTCGSCSGMFT 120 GPM +G + N+D+ I+ + AG + A++ E E + G+C+ T Sbjct: 180 AGPMTSG----MSNSDKVKIR-----QLYTAGK--IGRAELLEAESKSYHGAGTCTFYGT 228 Query: 121 ANSMNCLAEALGLALPGNGTIVATHADRKELFLGAGRLIVELAKRHYEQDDYSVLPRSIA 180 ANS L EA+GL LPG I R L A + ++ + Y+ + R I Sbjct: 229 ANSNQMLMEAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQ---YTPVGRMID 285 Query: 181 SKAAFENAMALDVSMGGSTNTVLHLLAAAHEAQVEFTMADIDRISRKVPCLCKVAPMTDK 240 K+ +AL + GGSTN LHL+A A A + D D++S VP + K+ P Sbjct: 286 EKSIVNAIVALHAT-GGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYP-NGT 343 Query: 241 YHIEDVHRAGGIVAVRAGLLDTSLPTVH---SKTMGEAIEKYDIRVSSDAAVHKLFRAAP 297 + H AGG V L+D L +H + +G+ + ++ + AA + R P Sbjct: 344 ADVNHFHAAGGTGFVLRELIDAGL--MHDDVTTILGQGLRQH-CKEPMLAAEGENGR--P 398 Query: 298 GGVPSQTAFSQAERYEHGDLDRSAGCIRDIEHAYSKDGGLAVLYGNIAEKGCIVKTAGVD 357 G V A +Q+ GD G + + ++ DGGL +L GN+ ++K + V Sbjct: 399 GIVTWHDAPAQS-----GD----EGVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVK 447 Query: 358 ESILKFSGKARVFESQDAAVEGILGDTVHAGDVVIIRYEGPK--GGPGMQEMLYPTSYIK 415 A VF SQ+A + + V +I ++GP+ G P + + ++ Sbjct: 448 PEHRVVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVITFQGPRANGMPELHALTPALGILQ 507 Query: 416 SKGLGKACALFTDGRFSGGSSGLVIG-HASPEAAEGGAIGLVEEGDMIDIDIPARTINLR 474 K G+ AL TDGR SG S + H +PE GG +G V +GD+I +D A T+ + Sbjct: 508 DK--GRHVALVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAK 565 Query: 475 VADDQLAQR 483 V + A R Sbjct: 566 VDATEWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,647,624 Number of extensions: 69673 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 8 Length of query: 523 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 433 Effective length of database: 1,193,720 Effective search space: 516880760 Effective search space used: 516880760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)