BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0012.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehyd... 369 e-103 gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 294 4e-81 gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehyd... 259 1e-70 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 93 3e-20 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 88 7e-19 >gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 369 bits (947), Expect = e-103, Method: Compositional matrix adjust. Identities = 194/252 (76%), Positives = 221/252 (87%) Query: 1 MQIAGQVFVVTGAASGLGAATARMLAQNGGKVVLADLQEEAGQKLAAELQGEFVRCDVTT 60 M+I G VF++TGAASGLGAATARM+A GGK VLADL +EAG+ LAAEL G FVRCDV++ Sbjct: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 Query: 61 EEDGLKAIAAAQQLGSLRGLVNCAGIAPGAKLIGKDGPHAMALFQKTLNINLVGTFNMAR 120 EED + AA+ LGSLRGLVNCAGIAP KL+GK GPH MALF KTLNINLVG+FNMAR Sbjct: 61 EEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMAR 120 Query: 121 LAADAMSRQEPMEQGERGVIINTASVAAFDGQIGQTAYSASKAAIVGMTLPMARDLARSG 180 AA+AM+R +E+GERGVII+TASVAA+DGQIGQTAY+ASK AIV MTLPMAR+L+RSG Sbjct: 121 FAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSG 180 Query: 181 IRVMTIAPGIFETPMMMSMPQEVLDSLGGAVPFPPRLGKAVEYAHLAKSIIENLMLNGET 240 IRVMTIAPGIFETPMM++MPQEV DSLG AVPFPPRLG+A EYA LA++IIEN+MLNGET Sbjct: 181 IRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGET 240 Query: 241 IRLDGAIRMPPK 252 IRLDGAIRM PK Sbjct: 241 IRLDGAIRMQPK 252 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 294 bits (753), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 154/254 (60%), Positives = 184/254 (72%), Gaps = 2/254 (0%) Query: 1 MQIAGQVFVVTGAASGLGAATARMLAQNGGKVVLADLQEEAGQKLAAEL--QGEFVRCDV 58 M + V+VVTGAASGLGAATA L + G KVVLAD+ AG+K+ A L FV DV Sbjct: 1 MAVKQGVYVVTGAASGLGAATAEQLIRAGAKVVLADVNPVAGEKMQATLGANARFVLTDV 60 Query: 59 TTEEDGLKAIAAAQQLGSLRGLVNCAGIAPGAKLIGKDGPHAMALFQKTLNINLVGTFNM 118 T+E + A +G++ GLVNCAGIAPG KL+GK G H + F + LNINLVG+FNM Sbjct: 61 TSETSAQEVFGVASSMGAVVGLVNCAGIAPGEKLLGKGGVHRLDSFMRALNINLVGSFNM 120 Query: 119 ARLAADAMSRQEPMEQGERGVIINTASVAAFDGQIGQTAYSASKAAIVGMTLPMARDLAR 178 ARLAA +S GERGVII+TAS+AAFDGQ+GQ AY+ASK +V MTLPMAR+LAR Sbjct: 121 ARLAAQHISAGPADATGERGVIIHTASIAAFDGQMGQVAYAASKGGVVSMTLPMARELAR 180 Query: 179 SGIRVMTIAPGIFETPMMMSMPQEVLDSLGGAVPFPPRLGKAVEYAHLAKSIIENLMLNG 238 GIRV+TIAPGI ETPMM+ MP+EVL+SL VPFPPRLGK E+A L I+EN LNG Sbjct: 181 HGIRVVTIAPGIIETPMMLGMPEEVLESLSRNVPFPPRLGKPSEFAGLVMHILENRYLNG 240 Query: 239 ETIRLDGAIRMPPK 252 E IR+DGA+RM K Sbjct: 241 EVIRMDGALRMSAK 254 >gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 259 bits (663), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 141/254 (55%), Positives = 180/254 (70%), Gaps = 2/254 (0%) Query: 1 MQIAGQVFVVTGAASGLGAATARMLAQNGGKVVLADLQEEAGQKLAAELQGEFVRCDVTT 60 MQ+ GQ +VTG ASGLGA T R L Q G +V + D+ EA Q LA EL+ VRCD+T Sbjct: 1 MQLQGQAALVTGGASGLGAETVRQLVQAGARVSILDVNMEAAQALADELRCHAVRCDITD 60 Query: 61 EEDGLKAIAAAQQL-GSLRGLVNCAGIAPGAKLIGKDG-PHAMALFQKTLNINLVGTFNM 118 + A+ AAQQ G+ R L+NCAGI +++GKDG P + F + +N+NL+GTFN+ Sbjct: 61 SDSVETALQAAQQTNGAPRILINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVNLIGTFNV 120 Query: 119 ARLAADAMSRQEPMEQGERGVIINTASVAAFDGQIGQTAYSASKAAIVGMTLPMARDLAR 178 RLAA M+ E + +GERGVI+ TASVAAFDGQ+GQ Y+ASK I MTLP+ARDLA+ Sbjct: 121 IRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQ 180 Query: 179 SGIRVMTIAPGIFETPMMMSMPQEVLDSLGGAVPFPPRLGKAVEYAHLAKSIIENLMLNG 238 GIRV+TIAPG+F TP++ +P+EV SL ++PFP RLGKA EYA LA I NL LNG Sbjct: 181 YGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNG 240 Query: 239 ETIRLDGAIRMPPK 252 E IRLDGA+R+ P+ Sbjct: 241 EVIRLDGALRLAPR 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 92.8 bits (229), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 83/251 (33%), Positives = 127/251 (50%), Gaps = 24/251 (9%) Query: 6 QVFVVTGAASGLGAATARMLAQNGGKVVLADLQEEAGQKLAAELQGEFVRC-----DVTT 60 +V +VTG A G+GA+ ++ LA G V++AD+ +A K A L R D+ + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 61 EEDGLKAIAAAQQ-LGSLRGLVNCAGIAPGAKLIGKDGPHAMALFQKTLNINLVGTFNMA 119 E A A ++ G LVN AG+A + D P + + +T+N+N+ G Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFL--DYP--LENWLQTMNVNVTGVLLAG 124 Query: 120 RLAADAMSRQEPMEQGERGVIINTASVAAFDGQIGQTAYSASKAAIVGMTLPMARDLARS 179 + AA M +Q+ G I+N +S++ G+TAY SKAA++G+T MA +LA+ Sbjct: 125 QHAARLMVKQQ------WGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQY 178 Query: 180 GIRVMTIAPGIFETPMMMSMPQE-VLDSLGGAVPFPPRLGKAVEYA----HLAKSIIENL 234 GI V ++APG +TPM +M E +S VP R G E A +LA E+ Sbjct: 179 GITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAE--ESA 235 Query: 235 MLNGETIRLDG 245 + G I +DG Sbjct: 236 YITGHVIPVDG 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 88.2 bits (217), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 74/231 (32%), Positives = 105/231 (45%), Gaps = 26/231 (11%) Query: 2 QIAGQVFVVTGAASGLGAATARMLAQNGGKVVLADLQEEAGQKLAAELQGEFV---RCDV 58 + G+V +VTGAASG+G ATAR + G +V+LAD A K+ L E DV Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDV 70 Query: 59 TTEEDGLKAIA-AAQQLGSLRGLVNCAGIAPGAKLIGKDGPHAMALFQKTLNINLVGTFN 117 + E + + A ++ G L LV+ AG+ + + P Q T N+N G F Sbjct: 71 SHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGN-VTEVSPEDWHRVQAT-NVN--GVFY 126 Query: 118 MARLAADAMSRQEPMEQGERGVIINTASVAAFDGQIGQTAYSASKAAIVGMTLPMARDLA 177 AR A P + RG I+N ASV+ +AY+ASK A+ +T MA D Sbjct: 127 GAREAL-------PHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFG 179 Query: 178 RSGIRVMTIAPGIFETPMMMSMPQEVLDSLGGAVPFPPRLGKAVEYAHLAK 228 R G+R+ + P + T M M + PP L K E L + Sbjct: 180 RKGVRINAVCPSLTHTAMTADMADD-----------PPLLDKFAERIALGR 219 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 752,445 Number of extensions: 30920 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 44 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)