BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0087.0005.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310513|ref|YP_003774605.1| small-conductance mechanosensit... 234 4e-63 >gi|300310513|ref|YP_003774605.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 358 Score = 234 bits (598), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 120/159 (75%), Positives = 135/159 (84%) Query: 97 MMPLMISQVAMRFVFYVLRRIFVKETTAGAFLLLFEKVFATLVWICVLLYITGMWPDLVD 156 M PL ++QVAMRFVFYVLR IFVK+ T GA L L EK A LVW+CV+L+ITG+WPD VD Sbjct: 1 MFPLTVAQVAMRFVFYVLRGIFVKDATVGALLHLSEKAIAVLVWLCVVLWITGLWPDFVD 60 Query: 157 YLDTTMLPLGRHKTSLLTILQATASVLVTLIIALWAGAMLEDRMMKLSSMHLSLRTVVAR 216 YLD T LPL RHK SLLT+LQ ASVLVTLIIALW G +LE+R+MK S MH SLRTVVAR Sbjct: 61 YLDGTTLPLARHKVSLLTMLQGAASVLVTLIIALWIGTVLENRLMKFSGMHSSLRTVVAR 120 Query: 217 MVRAILILVAILVSLSLVGIDLTVLSVFGGALGVGLGLG 255 M+RA+LIL+A LVSLSLVGIDLTVLSVFGGALGVG+GLG Sbjct: 121 MLRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLG 159 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.332 0.145 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 550,992 Number of extensions: 17157 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 1 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 129 (54.3 bits)