BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0086.0034.p (233 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311474|ref|YP_003775566.1| adenine-specific methylase/meth... 209 2e-55 >gi|300311474|ref|YP_003775566.1| adenine-specific methylase/methyltransferase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 209 bits (531), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 97/119 (81%), Positives = 111/119 (93%) Query: 1 MTPNNFATVRDILRYAITSFNTEKLFFGHGTNNAFDEAAYLILHTLKLPLDQLEPFLDAR 60 MTPNNF+TVRD+LRYA+T FNTE+LFFGHG++NA DEAAYLILHTLKLPLDQL+PFLDAR Sbjct: 1 MTPNNFSTVRDLLRYAVTRFNTEQLFFGHGSSNALDEAAYLILHTLKLPLDQLDPFLDAR 60 Query: 61 LLQGEIDNLLRVIMRRTEDRVPAAYITNEAWLGGYRFFVDERVIVPRSFIAELIPEHFS 119 LL EI ++RVI RR++DRVPAAYIT+EAWLG YRF+VDERVIVPRSFIAELIP+HFS Sbjct: 61 LLPDEIAAVMRVIERRSKDRVPAAYITHEAWLGAYRFYVDERVIVPRSFIAELIPQHFS 119 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 682,791 Number of extensions: 25327 Number of successful extensions: 82 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 82 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 151 Effective length of database: 1,231,600 Effective search space: 185971600 Effective search space used: 185971600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)