BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0086.0030.p (193 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 277 4e-76 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 69 3e-13 gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/sign... 64 8e-12 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 64 1e-11 gi|300311601|ref|YP_003775693.1| glutamine ABC transporter perip... 61 6e-11 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 277 bits (708), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 137/147 (93%), Positives = 144/147 (97%) Query: 25 NVAKLLAAKLGLKPEFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSS 84 +VAKLLAAKLG+KP FTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSS Sbjct: 66 DVAKLLAAKLGVKPVFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSS 125 Query: 85 AQLIVRKDEKREFKSLEDLKGKKLGLGQGTNFEQKAKSVPGIDVKTYPGSPEYLADLASG 144 AQLIVRKDEKREFKSLEDLKGKKLGLGQGTNFEQKAK+VPGIDV+TYPGSPEYLADLA+G Sbjct: 126 AQLIVRKDEKREFKSLEDLKGKKLGLGQGTNFEQKAKAVPGIDVRTYPGSPEYLADLAAG 185 Query: 145 RIDAALNDSLLVSYLLKSSNLPLKGGS 171 RIDAALNDSLLV Y+LKS+NLPLK GS Sbjct: 186 RIDAALNDSLLVGYILKSTNLPLKAGS 212 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 68.9 bits (167), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 46/154 (29%), Positives = 84/154 (54%), Gaps = 5/154 (3%) Query: 25 NVAKLLAAKLGLKPEFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSS 84 ++ K LA K+G++ +F T + G L G D++I+ + ITDER+K+ FS+PY +++ Sbjct: 75 DIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDRDLLISAITITDERKKSVAFSEPYFVAT 134 Query: 85 AQLIVRKDEKREFKSLEDLKGKKLGLGQGTNFEQKAKSVPGID---VKTYPGSPEYLADL 141 Q I + +EDLK +G T+ ++ + V G + +K + +P L +L Sbjct: 135 -QTIALPAADTKVSKMEDLKPLTVGTQSATSGDELVQQVLGKNSAKIKRFDSTPLALKEL 193 Query: 142 ASGRIDAALNDSLLV-SYLLKSSNLPLKGGSIPA 174 SG +DA + D +V +Y+ + N L+ + P+ Sbjct: 194 ESGGVDAVVADEPVVKNYIANNPNSKLRTVTDPS 227 >gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 258 Score = 63.9 bits (154), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 42/135 (31%), Positives = 63/135 (46%), Gaps = 1/135 (0%) Query: 33 KLGLKPEFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQLIVRKD 92 K G T ++ ++ L K D+I + T+ R + DFSDP L V Sbjct: 70 KAGFSVNIQQTPFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSYGEGLFVNAQ 129 Query: 93 EKREFKSLEDLKGKKLGLGQGTNFEQKAKSVPGI-DVKTYPGSPEYLADLASGRIDAALN 151 + + +K+L+DLKG+ +G GT F G +VKTY + + D+A GRI A Sbjct: 130 DGKSYKNLDDLKGEIVGAQVGTVFVDALNKKGGFKEVKTYDSVADMMRDVALGRIKAGFG 189 Query: 152 DSLLVSYLLKSSNLP 166 D +V+Y L P Sbjct: 190 DRPIVAYQLAQGANP 204 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 40/135 (29%), Positives = 69/135 (51%), Gaps = 3/135 (2%) Query: 30 LAAKLGLKPEFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQLIV 89 +A +G+ + +++GI+ L DV + + I DER+K DFSD Y S L+V Sbjct: 58 IAKDIGVTYKLQPMDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMV 117 Query: 90 RKDEKREFKSLEDLKGKKLGLGQGTN-FEQKAKSVPGIDVKTYPGSPEYLADLASGRIDA 148 KS+ DL GK L + GT+ F+ + ++ +P +L +GR+DA Sbjct: 118 PVGS--SIKSVADLAGKALAVKSGTSAFDYAKANFKATELHQFPNIDNAYLELQTGRVDA 175 Query: 149 ALNDSLLVSYLLKSS 163 A++D+ V Y + ++ Sbjct: 176 AMHDTPNVLYYIATA 190 >gi|300311601|ref|YP_003775693.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 60.8 bits (146), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 38/124 (30%), Positives = 69/124 (55%), Gaps = 3/124 (2%) Query: 30 LAAKLGLKPEFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQLIV 89 LA +G K ++ ++ G++ GL + ++D+ + + +TDER+K +FSDPY ++V Sbjct: 62 LAKTMGKKVQWIDIDFKGLIPGLVSNRFDIAASAIYMTDERRKVVNFSDPYYRGGLAVLV 121 Query: 90 RKDEKREFKSLEDL-KGKKLGLGQGTNFEQKAK-SVPGIDVKTYPGSPEYLADLASGRID 147 R+D+ K EDL KGK++ + GT K + PG++ + LA+GR++ Sbjct: 122 RRDDN-SIKVPEDLNKGKRVSVQVGTKSVGYLKDNFPGVERVEVEKNQAMFDLLATGRVN 180 Query: 148 AALN 151 A + Sbjct: 181 AVVT 184 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 529,273 Number of extensions: 18381 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 65 Number of HSP's successfully gapped: 8 Length of query: 193 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 113 Effective length of database: 1,241,070 Effective search space: 140240910 Effective search space used: 140240910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)