BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0085.0012.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 57 5e-10 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 54 6e-09 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 54 6e-09 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 54 8e-09 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 57.4 bits (137), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 41/126 (32%), Positives = 59/126 (46%), Gaps = 6/126 (4%) Query: 15 VTGGERVDVLG---DGVYVRPALVCMPGQTAIMHHETFAPILYVVKYDDLADAIALNNAV 71 +TGG+R+ G D Y L + G ETF P+ + ++ + IA NA Sbjct: 361 ITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANAT 420 Query: 72 PQGLSSAIFTNDVREAETFVSAVGSDCGLANVNIGTSGAEIGGAFGGEKETGGGRESGSD 131 P GL++ ++ DVR A+ S G+ VN G AE FGG KE+G GRE Sbjct: 421 PFGLAAYFYSTDVRRIHRVTDALES--GIVGVNEGALAAE-AAPFGGVKESGYGREGSVH 477 Query: 132 AWKNYM 137 +Y+ Sbjct: 478 GLDDYL 483 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 53.9 bits (128), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 38/140 (27%), Positives = 71/140 (50%), Gaps = 9/140 (6%) Query: 1 MQSALQQARGQGGEV-TGGERVDVLGDGVYVRP-ALVCMPGQTAIMHHETFAPILYVVKY 58 +++ + A QG ++ TGG+R+ G + +P L +P Q M+ E F P+ + + Sbjct: 334 LEALIDDAVSQGAQLRTGGKRIG--NKGYFFQPTVLTNLPLQARAMNEEPFGPLALINSF 391 Query: 59 DDLADAIALNNAVPQGLSSAIFTNDVREAETFVSAVGSDCGLANVN-IGTSGAEIGGAFG 117 + L + I N +P GL++ F + V + G+ +N +G + E+ FG Sbjct: 392 ETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARV--ESGMLTINHLGLALPEL--PFG 447 Query: 118 GEKETGGGRESGSDAWKNYM 137 G K++G G E G++A + Y+ Sbjct: 448 GIKDSGYGSEGGTEAIEAYI 467 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 53.9 bits (128), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 43/121 (35%), Positives = 57/121 (47%), Gaps = 9/121 (7%) Query: 1 MQSALQQARGQGGE-VTGGERVDVLGDGVYVRPALVC-MPGQTAIMHHETFAPILYVVKY 58 +Q + A QG E VTGG R+ G + P L+ +P +M E F PI V + Sbjct: 334 VQGLIDDALAQGSELVTGGARLGQRGH--FYAPTLITGVPPSARVMQEEPFGPIAAVTPF 391 Query: 59 DDLADAIALNNAVPQGLSSAIFTNDVREAETFVSAVGSDCGLANVNIGTSG-AEIGGAFG 117 +AIA+ NA+P GL+S +FT + A V D VNI SG A FG Sbjct: 392 SQAEEAIAMANALPLGLASYVFTQSLDTAHR----VSRDLQAGMVNINHSGMAHPELPFG 447 Query: 118 G 118 G Sbjct: 448 G 448 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 53.5 bits (127), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 38/121 (31%), Positives = 58/121 (47%), Gaps = 3/121 (2%) Query: 27 GVYVRPALVC-MPGQTAIMHHETFAPILYVVKYDDLADAIALNNAVPQGLSSAIFTNDVR 85 G Y PAL+ +P + + E F P+L + ++D A AIAL N GL ++++T D Sbjct: 358 GFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGG 417 Query: 86 EAETFVSAVGSDCGLANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKNYMRRATNTIN 145 A+ S G +N +G + FGG K +G GRE G +A + T I Sbjct: 418 RQMRLARAIRS--GQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIR 475 Query: 146 Y 146 + Sbjct: 476 H 476 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 410,363 Number of extensions: 12766 Number of successful extensions: 42 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 5 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 127 (53.5 bits)