BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0085.0010.p (97 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 69 8e-14 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 67 3e-13 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 64 2e-12 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 60 2e-11 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 58 1e-10 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 68.9 bits (167), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 39/93 (41%), Positives = 52/93 (55%), Gaps = 1/93 (1%) Query: 1 VTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVI 60 V +E G +L +G EV+E I +C A G+ Q G + S PG + PLGV GVI Sbjct: 94 VARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLLPST-PGRLSIARRAPLGVIGVI 152 Query: 61 TAFNFPVAVWAWNAALALICGNSVVWKPSEKTP 93 + FNFP+ + A AL GN+VV KP +TP Sbjct: 153 SPFNFPLILTLRTVAPALAAGNAVVVKPDLRTP 185 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 66.6 bits (161), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 38/93 (40%), Positives = 50/93 (53%) Query: 1 VTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVI 60 +T E GK+ S+ GEV ++I +FA G+ + L + G PLGV I Sbjct: 89 ITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDNIGGGIDNWNLRQPLGVTAGI 148 Query: 61 TAFNFPVAVWAWNAALALICGNSVVWKPSEKTP 93 T FNFPV V W A +A+ GNS V KPSE+ P Sbjct: 149 TPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 63.9 bits (154), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 36/93 (38%), Positives = 46/93 (49%) Query: 1 VTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVI 60 + +E GK S+ GEV I+I +FA G+ + L G G LGV I Sbjct: 87 IVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQIARGIDAWSMRQALGVVAGI 146 Query: 61 TAFNFPVAVWAWNAALALICGNSVVWKPSEKTP 93 T FNFP V W +A+ CGN V KPSE+ P Sbjct: 147 TPFNFPAMVPMWMFPIAIACGNCFVLKPSERDP 179 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 60.5 bits (145), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 33/93 (35%), Positives = 51/93 (54%) Query: 1 VTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVI 60 +T+E GK L + GEV +++ + A ++ G + G + + PLGV I Sbjct: 92 ITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAENVAGGVDVYTLYQPLGVGAGI 151 Query: 61 TAFNFPVAVWAWNAALALICGNSVVWKPSEKTP 93 TAFNFPV + + +A+ CGN+ V KPSE+ P Sbjct: 152 TAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDP 184 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 57.8 bits (138), Expect = 1e-10, Method: Composition-based stats. Identities = 35/93 (37%), Positives = 50/93 (53%) Query: 1 VTQEAGKILSEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVCGVI 60 +++E GK L+E GEV ++ + + +G I + G RMM P+GV I Sbjct: 97 ISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAI 156 Query: 61 TAFNFPVAVWAWNAALALICGNSVVWKPSEKTP 93 T +NFP A+ A A AL G +VV KP+E TP Sbjct: 157 TPWNFPAAMIARKIAPALAAGCTVVCKPAEDTP 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 277,668 Number of extensions: 8311 Number of successful extensions: 55 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 55 Number of HSP's successfully gapped: 6 Length of query: 97 Length of database: 1,619,870 Length adjustment: 66 Effective length of query: 31 Effective length of database: 1,307,360 Effective search space: 40528160 Effective search space used: 40528160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)