BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0085.0004.p (605 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo... 1071 0.0 gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo... 651 0.0 >gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 898 Score = 1071 bits (2771), Expect = 0.0, Method: Compositional matrix adjust. Identities = 521/556 (93%), Positives = 530/556 (95%), Gaps = 17/556 (3%) Query: 1 MRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHFWHAPTEDHGGDLLYIQGHSS 60 MRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHFWHAPTEDHGGDLLYIQGHSS Sbjct: 92 MRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHFWHAPTEDHGGDLLYIQGHSS 151 Query: 61 PGIYARXYIQGHSSPGIYA---LEGRLTEDQLVHFRREADGHGLSSYPHPKLMPEFWQFP 117 PGIYA LEGRLTEDQL+HFRREADGHGLSSYPHPKLMPEFWQFP Sbjct: 152 --------------PGIYARAFLEGRLTEDQLIHFRREADGHGLSSYPHPKLMPEFWQFP 197 Query: 118 TVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESMGAIGMAGREKL 177 TVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESMGAIGMAGREKL Sbjct: 198 TVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESMGAIGMAGREKL 257 Query: 178 NNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSGWDELLAKDKEGIL 237 NNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSGWDELLAKDK+GIL Sbjct: 258 NNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSGWDELLAKDKDGIL 317 Query: 238 QKVMMETVDGEYQNYKAKDGAFVRKHFFGKHPKLLEMVSKMSDDDIWRLTRGGHDPHKIY 297 QKVMMETVDGEYQNYKAKDGA+VRKHFFGKHPKLLEMVSKMSDDDIWRLTRGGHDPHKIY Sbjct: 318 QKVMMETVDGEYQNYKAKDGAYVRKHFFGKHPKLLEMVSKMSDDDIWRLTRGGHDPHKIY 377 Query: 298 AAFKVAQDSKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAIKAMRDRFQLPISD 357 AAFKVAQ+SKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAI+AMRDRFQLPISD Sbjct: 378 AAFKVAQESKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAIRAMRDRFQLPISD 437 Query: 358 AELPNIPFFKPSADTPEMQYLHERRKALGGYLPQRRPQADEKLAVPELSAFQAMLEPTAE 417 A+LPNIPFFKPS D PEMQYLHERRKALGGYLPQRR QADEKL VPELSAFQAMLEPTAE Sbjct: 438 ADLPNIPFFKPSDDAPEMQYLHERRKALGGYLPQRRVQADEKLPVPELSAFQAMLEPTAE 497 Query: 418 GREISTTAAYSRVLTALLRDSSLGQRVVPIMVDESRTFGMEGLFRQIGIFSQVGQLYEPV 477 GREISTTAAYSRVLTALLRD +LG RVVPIMVDESRTFGMEGLFRQIGIFSQVGQLYEPV Sbjct: 498 GREISTTAAYSRVLTALLRDPNLGPRVVPIMVDESRTFGMEGLFRQIGIFSQVGQLYEPV 557 Query: 478 DKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRIMIPFYTYYSMFGLQRIG 537 DKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNR+MIPFYTYYSMFGLQRIG Sbjct: 558 DKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRVMIPFYTYYSMFGLQRIG 617 Query: 538 DLAWAAGDMRARXLIC 553 DLAWAAGDMRAR + Sbjct: 618 DLAWAAGDMRARGFLI 633 >gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 651 bits (1680), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/564 (56%), Positives = 402/564 (71%), Gaps = 18/564 (3%) Query: 1 MRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHFWHAPTEDHGGDLLYIQGHSS 60 MRWNA+AMVV+ANR G+LGGH++SFAS A++ +GFNHF+ A E GD++Y Q HS+ Sbjct: 94 MRWNALAMVVRANRAYGELGGHIASFASAADLFEVGFNHFFRARDEQFAGDVVYFQPHSA 153 Query: 61 PGIYARXYIQGHSSPGIYALEGRLTEDQLVHFRREADG-----HGLSSYPHPKLMPEFWQ 115 PGIYAR + LEG L+ED L ++RRE + GLSSYPHP LMP+FWQ Sbjct: 154 PGIYARAF-----------LEGALSEDDLAYYRREIEAKRVGRQGLSSYPHPWLMPQFWQ 202 Query: 116 FPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESMGAIGMAGRE 175 FPT SMG+GP+ AIYQARFL+Y+ RG+ + RKVW GDGEMDEPES+ A+ +A RE Sbjct: 203 FPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWGVFGDGEMDEPESLAALSLASRE 262 Query: 176 KLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSGWDELLAKDKEG 235 KL+NL+ VVNCNLQRLDGPVRGNG I+ ELE+ F GAGWNV+K++WGS WD L A+DK+G Sbjct: 263 KLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGAGWNVIKLLWGSDWDGLFARDKDG 322 Query: 236 ILQKVMMETVDGEYQNYKAKDGAFVRKHFFGKHPKLLEMVSKMSDDDIWRLTRGGHDPHK 295 L + TVDG+ Q + A DGAF R+HFFG+ P + + ++D++I RL RGGHD K Sbjct: 323 ELVDALNRTVDGQLQTFAANDGAFNRQHFFGQTPGTRAIGATLTDEEINRLRRGGHDMKK 382 Query: 296 IYAAFKVAQDSKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAIKAMRDRFQLPI 355 IY+A++ A + QPTVILA++ KGYG G AGE + T H KKLD+E++ RDRF+LP+ Sbjct: 383 IYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEESLLQFRDRFKLPL 442 Query: 356 SDAELPNIPFFKPSADTPEMQYLHERRKALGGYLPQRRPQADEKLAVPELSAFQAMLEPT 415 SD + + F+KP+ D+PEM++LH RR AL GYLP RR + AVP L+A + Sbjct: 443 SDEQCRALAFYKPADDSPEMKHLHARRAALSGYLP-RRQAGQARRAVPPLAA-HSKFALE 500 Query: 416 AEGREISTTAAYSRVLTALLRDSSLGQRVVPIMVDESRTFGMEGLFRQIGIFSQVGQLYE 475 A+G+E+S+T A R L LL+D GQ VVPI+ DE+RTFGM LFRQ+GI+S GQLYE Sbjct: 501 ADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTFGMANLFRQVGIYSSQGQLYE 560 Query: 476 PVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRIMIPFYTYYSMFGLQR 535 P D ++YYRE K GQIL+EGI EAG +SSW AAAT+YS + M+PFY YYSMFG QR Sbjct: 561 PEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYSVHGTPMLPFYIYYSMFGFQR 620 Query: 536 IGDLAWAAGDMRARXLICARLPDR 559 IGDL WAA D RAR + R Sbjct: 621 IGDLIWAAADQRARGFLIGATSGR 644 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,920,910 Number of extensions: 80975 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 2 Length of query: 605 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 514 Effective length of database: 1,188,985 Effective search space: 611138290 Effective search space used: 611138290 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)