BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0084.0005.p (452 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 553 e-159 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 94 2e-20 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 89 5e-19 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 87 2e-18 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 553 bits (1426), Expect = e-159, Method: Compositional matrix adjust. Identities = 266/332 (80%), Positives = 302/332 (90%) Query: 1 VQILALALLILCSAFFSMTETALMAANRHRLRHLAKHGNRRAIALLWLLERTDRLLSLIL 60 VQ+LAL +LIL SAFFSMTETALMAANRHRLRHLAK GNR AI +LWLL+RT+RLLSL+L Sbjct: 8 VQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLVL 67 Query: 61 IANTLINALATALVTALAIAAFGNHEKVITLATACVAGLLIIFAEIVPKIIGATYPERIS 120 IANT INALATALVTALAIAAFGNH++VIT ATACVA LLIIFAEIVPK+IGATYPERIS Sbjct: 68 IANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGATYPERIS 127 Query: 121 LFTSYILKPLMALAKPLLWAVNIVVSTILRIIHIKASGRSHDQRLSPEELRSIVLEGGNF 180 LFT +ILKPLM +AKPL+W VN+ VS ILR++ IK R+ +QR+SPEELRSIVLEGG+F Sbjct: 128 LFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIVLEGGSF 187 Query: 181 IPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGE 240 IPQKHKSILLNLFDLEKISVEDVMTPR+QVEALNL+ +D+I +QLATCYHNKLPV+EGE Sbjct: 188 IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGE 247 Query: 241 INQIVGILHVRKAIMLFSQDEEITSEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGI 300 IN IVGILHVRK + LF ++EEI+ ++IR LLSAPY++P +TDVFTQLQYFQENHERLG+ Sbjct: 248 INHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGV 307 Query: 301 IVDEYGEVQGLVTLEDIIEEMIGEFTTSVPAS 332 +VDEYGEVQGLVTLEDIIEEMIGEFTTS P S Sbjct: 308 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGS 339 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 84/358 (23%), Positives = 167/358 (46%), Gaps = 35/358 (9%) Query: 13 SAFFSMTETALMAANRHRLRHLAKHGNRRAIALLWLLERTDRLLSLILIANTLINALAT- 71 SAF S E ++ A+ + RL ++K G A +L L + +++ I +NA+A Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTVVQIG---LNAVAIL 73 Query: 72 --------------ALVTALAIAAFGNHEKVITLATACVAGLLIIFAEIVPKIIGATYPE 117 ++++ I + + ++ V I+FA+++PK + PE Sbjct: 74 GGVVGEPALTPHMREIISSFYIGPWLENAS-FAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 118 RISLFTSYILKPLM---ALAKPLLWAVNIVVSTILRIIHIKASGRSHDQRLSPEELRSIV 174 R ++ ++KP++ L KPL+W N + I + + + + ++P E+ ++V Sbjct: 133 RAAV---ALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLP---QVRQEVITPNEIHAMV 186 Query: 175 LEG---GNFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYH 231 G G + Q+H ++ N+F+LE V MTPR + L+ S IR ++ H Sbjct: 187 DAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPH 245 Query: 232 NKLPVYEGEINQIVGILHVRKAIMLFSQDEEITSEEIRALLSAPYFIPQDTDVFTQLQYF 291 ++ V +G I+ ++G + + + + E+ + + + L+ P IP +++ L+ Sbjct: 246 SRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERM 304 Query: 292 QENHERLGIIVDEYGEVQGLVTLEDIIEEMIGEF--TTSVPASGKRRKPNSYDLQGGT 347 + E L I+V+EY V G+++ D+ ++G T RR NS+ + G T Sbjct: 305 KAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAITEEEQQIVRRDENSWLMDGLT 362 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 89.4 bits (220), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 42/146 (28%), Positives = 88/146 (60%), Gaps = 4/146 (2%) Query: 179 NFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYE 238 N I S++ +F + ++V D+M PR+Q++ +++S I+ ++ H++ P + Sbjct: 47 NLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVD 106 Query: 239 GEINQIVGILHVRKAIMLFSQDEEITSEEIRALLSAPYFIPQDTDVFTQLQYFQENHERL 298 GE +++VGIL + + ++++ S ++R +L FIP+ + L+ F+ N + Sbjct: 107 GERDKVVGILLAKDLLRYYAEE----SFDVRDMLRPAIFIPESKRLNVLLRDFRANRNHM 162 Query: 299 GIIVDEYGEVQGLVTLEDIIEEMIGE 324 ++VDEYG V GL+T+ED++E+++G+ Sbjct: 163 AVVVDEYGGVAGLITIEDVLEQIVGD 188 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 79/341 (23%), Positives = 152/341 (44%), Gaps = 23/341 (6%) Query: 3 ILALALLILCSAFFSMTETALMAANRHRLRHLAKHGNRRAIALLWLLERTDRLLSLILIA 62 I+ L+ + FF E +L+ + R+R +AK R L + + D LS + Sbjct: 7 IVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYLSACQLG 66 Query: 63 NTLIN---------ALATALVTALAIAAFGNHEKV----ITLATACVAGLLIIFAEIVPK 109 TL + A A L ++A N E + A ++ L I+ E+ PK Sbjct: 67 ITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAPK 126 Query: 110 IIGATYPERISLFTSYILKPLMALAKPLLWAVNIVVSTILRIIHIKASGRSHDQRLSPEE 169 + PE++ L+ + L PL+W +N + +LR+ + A G HD S +E Sbjct: 127 SMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGA-GHGHDAHYSSDE 185 Query: 170 LRSIVLEGGNFIPQKHK------SILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIR 223 L+ ++L GN + K ++L + ++ V D+M P +++ AL S++E Sbjct: 186 LK-LILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENL 244 Query: 224 NQLATCYHNKLPVYEGEINQIVGILHVRKAIMLFSQDEEITSEEIRALLSAPYFIPQDTD 283 + + +++ P Y+ E Q++G++H+ K + L QD + ++ L +I Sbjct: 245 DIIYRNRYSRYPYYDAERQQVLGLVHL-KDVFLAQQDGRAIA-NLKDYLRPVQYISPALP 302 Query: 284 VFTQLQYFQENHERLGIIVDEYGEVQGLVTLEDIIEEMIGE 324 L+ F+ +I + G +TL++++ ++GE Sbjct: 303 ALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGE 343 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 896,367 Number of extensions: 30926 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 116 Number of HSP's successfully gapped: 4 Length of query: 452 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 364 Effective length of database: 1,203,190 Effective search space: 437961160 Effective search space used: 437961160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)