BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0082.0044.p (509 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-bind... 960 0.0 gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subu... 243 2e-65 gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [... 159 5e-40 gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [H... 147 2e-36 >gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-binding specificity subunit [Herbaspirillum seropedicae SmR1] Length = 767 Score = 960 bits (2482), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/516 (92%), Positives = 497/516 (96%), Gaps = 8/516 (1%) Query: 1 MGALLAGTKYRGDFEQRLKAVLKQLKDAPNGILFIDEIHTIIGAGSASGGTLDASNLLKP 60 MGALLAGTKYRGDFEQRLKAVLKQLKD+PNGILFIDEIHTIIGAGSASGGTLDASNLLKP Sbjct: 253 MGALLAGTKYRGDFEQRLKAVLKQLKDSPNGILFIDEIHTIIGAGSASGGTLDASNLLKP 312 Query: 61 ALSSGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQTIQILRGLKSKFEEHH 120 ALSSGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQT+QILRGLKSKFEEHH Sbjct: 313 ALSSGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQTVQILRGLKSKFEEHH 372 Query: 121 GVKYSASALTSAAELAARFINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTIGKSEIEEI 180 GVKYSASALTSAAELAARFINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTIGK EIEEI Sbjct: 373 GVKYSASALTSAAELAARFINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTIGKPEIEEI 432 Query: 181 ISKIARIPPQSVNQDDRTKLQTIDRDLKNVVFGQDPAIEALASAIKM---GVGKTE--VA 235 ISKIARIPPQSVNQDDR KLQTIDRDLKNVVFGQDPAIEAL+SAIKM G+GKT+ + Sbjct: 433 ISKIARIPPQSVNQDDRAKLQTIDRDLKNVVFGQDPAIEALSSAIKMARAGLGKTDKPIG 492 Query: 236 KQL--AXTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLL 293 L TGVGKTEVAKQLAFILGIEL+RFDMSEYMERHAVSRLIGAPPGYVGFDQGGLL Sbjct: 493 SFLFSGPTGVGKTEVAKQLAFILGIELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLL 552 Query: 294 TEAITKKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVIIVMTTNAGA 353 TEA+ KKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVII+MTTNAGA Sbjct: 553 TEAVNKKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVIIIMTTNAGA 612 Query: 354 ESLQKRSIGFTDKKEAGDEMADIKRMFTPEFRNRLDSIISFRALDEDIILRVVDKFLMQL 413 ESLQKR+IGFT+KKEAGDEMADIKRMFTPEFRNRLD+IISFR LDE+IILRVVDKFLMQL Sbjct: 613 ESLQKRTIGFTEKKEAGDEMADIKRMFTPEFRNRLDAIISFRPLDEEIILRVVDKFLMQL 672 Query: 414 EEQLHEKKVEAVFSDALRKFLSKKGFDPLMGARPMSRLIQDMIRKALADELLFGRLVAGG 473 EEQLHEKKVEAVFSD+LRKFL KKGFDPLMGARPMSRLIQD+IRKALADELLFG+LV+GG Sbjct: 673 EEQLHEKKVEAVFSDSLRKFLGKKGFDPLMGARPMSRLIQDLIRKALADELLFGKLVSGG 732 Query: 474 RVTVELDDKDQVKLEFPEGDDPTPPPEEPQEKLEVE 509 RVTVE+DDKDQ+KLEFPEGDD + PPEEPQEKLEVE Sbjct: 733 RVTVEMDDKDQIKLEFPEGDD-SSPPEEPQEKLEVE 767 >gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subunit [Herbaspirillum seropedicae SmR1] Length = 861 Score = 243 bits (620), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 146/348 (41%), Positives = 215/348 (61%), Gaps = 18/348 (5%) Query: 147 DKAIDVIDEAGAAQRILPKSKQKKT-IGKSEIEEIISKIARIPPQSVNQDDRTKLQTIDR 205 + A++V ++ AA K K +T +G EI EI+++ IP + Q +R KL ++ Sbjct: 510 EAALEVQNKKDAAGVKTEKPKLVRTQVGAEEIAEIVARATGIPVSRMMQGEREKLLHMED 569 Query: 206 DLKNVVFGQDPAIEALASAIKMG-VGKTEVAKQ------LAXTGVGKTEVAKQLA-FILG 257 L V GQD AI A++ AI+ G + +K L TGVGKTE+ K LA ++ Sbjct: 570 VLHERVVGQDEAIVAVSDAIRRSRAGLGDPSKPYGSFMFLGPTGVGKTELCKALASYLFD 629 Query: 258 IE--LIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKKPHAVLLLDEIEKAHP 315 E +IR DMSE+ME+H+V+RLIGAPPGYVG+++GG LTEA+ +KP++V+LLDEIEKAHP Sbjct: 630 TEEAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHP 689 Query: 316 DIFNILLQVMDHGTLTDNNGRKTDFRNVIIVMTTNAGAESLQKRSIGFTDKKEAGDE--- 372 D+FN+LLQV+D G +TD GR DF+N +IVMT+N G+ +Q D A + Sbjct: 690 DVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKIQ----SMEDSDPALVKLAV 745 Query: 373 MADIKRMFTPEFRNRLDSIISFRALDEDIILRVVDKFLMQLEEQLHEKKVEAVFSDALRK 432 MA+++ F PEF NR+D I+ F ALD I + L LE++L + ++ S+A + Sbjct: 746 MAEVRTHFRPEFINRVDEIVVFHALDAKNIGAIAKIQLRILEQRLAKLEIGLEISEAGLQ 805 Query: 433 FLSKKGFDPLMGARPMSRLIQDMIRKALADELLFGRLVAGGRVTVELD 480 +++ GFDP+ GARP+ R IQ I L+ ++L G+ V V++D Sbjct: 806 KIAEAGFDPVYGARPLKRAIQQEIENPLSKQILAGKFGPKDVVRVDVD 853 Score = 201 bits (511), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 101/168 (60%), Positives = 128/168 (76%), Gaps = 2/168 (1%) Query: 1 MGALLAGTKYRGDFEQRLKAVLKQL-KDAPNGILFIDEIHTIIGAGSASGGTLDASNLLK 59 M ALLAG KYRG+FE+RLKAVLK++ +D I+FIDE+HT++GAG A G +DA N+LK Sbjct: 242 MAALLAGAKYRGEFEERLKAVLKEIAQDEGQTIVFIDELHTMVGAGKAEG-AMDAGNMLK 300 Query: 60 PALSSGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQTIQILRGLKSKFEEH 119 PAL+ G+L C+GATT E+R EKD AL RRFQKI V+EP+VE TI ILRGL+ K+E H Sbjct: 301 PALARGELHCVGATTLDEYRQYIEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVH 360 Query: 120 HGVKYSASALTSAAELAARFINDRHLPDKAIDVIDEAGAAQRILPKSK 167 HGV + A+ +AAEL+ R+I DR LPDKAID+IDEA + +I SK Sbjct: 361 HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKIEIDSK 408 >gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 159 bits (402), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 82/159 (51%), Positives = 109/159 (68%), Gaps = 2/159 (1%) Query: 1 MGALLAGTKYRGDFEQRLKAVLKQLKDAPNGI-LFIDEIHTIIGAGSASGGTLDASNLLK 59 MG L AG +G+FE RLK V+ ++K +P+ I LFIDE HT+IGAG +G DA+NLLK Sbjct: 277 MGLLQAGASVKGEFENRLKGVIDEVKKSPHPIILFIDEAHTMIGAGGQAGQN-DAANLLK 335 Query: 60 PALSSGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQTIQILRGLKSKFEEH 119 PAL+ G+L+ I ATT++E++ FEKD AL+RRFQ I V EPT E +LR + E+H Sbjct: 336 PALARGELRTIAATTWSEYKKYFEKDAALARRFQVIKVEEPTEEVACAMLRAMAPLMEKH 395 Query: 120 HGVKYSASALTSAAELAARFINDRHLPDKAIDVIDEAGA 158 GV+ A+T A L+ R+I+ R LPDKAI V+D A A Sbjct: 396 FGVRVYDEAITEAVRLSHRYISGRQLPDKAISVLDTACA 434 Score = 127 bits (320), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 99/313 (31%), Positives = 168/313 (53%), Gaps = 19/313 (6%) Query: 177 IEEIISKIARIPPQSVNQDDRTKLQTIDRDLKNVVFGQDPAIEALASAIKMGVGKTE--- 233 + EI++ IP + +D+ + +D LK V GQ A A+A ++ + Sbjct: 562 VAEIVAGWTGIPLGKMVKDEIKTVLGLDALLKERVLGQPQATAAVAQRVRTSRANLDDPG 621 Query: 234 ----VAKQLAXTGVGKTEVAKQLAFIL-GIE--LIRFDMSEYMERHAVSRLIGAPPGYVG 286 V + +GVGKTE A LA +L G E LI +MSEY E H VS L G+PPGYVG Sbjct: 622 KPKGVFLFVGPSGVGKTETALALADVLYGGERKLITINMSEYQEAHTVSGLKGSPPGYVG 681 Query: 287 FDQGGLLTEAITKKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVIIV 346 + +GG+LTEA+ + P++V+LLDE+EKAHPD+ + QV D G + D GR+ DF+N II+ Sbjct: 682 YGEGGVLTEAVRRNPYSVVLLDEVEKAHPDVLELFFQVFDKGVMDDGEGREIDFKNTIII 741 Query: 347 MTTNAGAESLQKRSIGFTDKK-EAGDEM-----ADIKRMFTPEFRNRLDSIISFRALDED 400 +T+N + L + + + + DE+ + + F P F RL +I + + ++ Sbjct: 742 LTSNVASSQLMQACLNKSAAELPTPDELDVLIRPQLVKAFKPAFLGRL-KVIPYYPISDE 800 Query: 401 IILRVVDKFLMQLEEQLHEK-KVEAVFSDALRKFLSKKGFDPLMGARPMSRLIQDMIRKA 459 ++ +++ L ++ ++ E K E + AL + + + + GAR + ++ + Sbjct: 801 VLEQIIKLKLGRIANRIAENHKAEFSYDKALVEAVLARCTEVDSGARNVDNILNGSLLPE 860 Query: 460 LADELLFGRLVAG 472 +A+ +L R+ G Sbjct: 861 IAESVL-ARMAEG 872 >gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [Herbaspirillum seropedicae SmR1] Length = 889 Score = 147 bits (370), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 78/159 (49%), Positives = 105/159 (66%), Gaps = 2/159 (1%) Query: 1 MGALLAGTKYRGDFEQRLKAVLKQLK-DAPNGILFIDEIHTIIGAGSASGGTLDASNLLK 59 MG L AG +G+FE RLK V+ ++K A ILFIDE HT+IGA +G + DA+NLLK Sbjct: 273 MGLLQAGASVKGEFESRLKTVIDEVKKSAYPIILFIDEAHTMIGAAGQAGQS-DAANLLK 331 Query: 60 PALSSGQLKCIGATTYTEFRGVFEKDHALSRRFQKIDVNEPTVEQTIQILRGLKSKFEEH 119 PAL+ G+L+ I ATT++E++ FEKD AL+RRFQ I V EP + +LR + E+H Sbjct: 332 PALARGELRTIAATTWSEYKKYFEKDAALARRFQVIKVEEPGEDVACAMLRAMVPLMEQH 391 Query: 120 HGVKYSASALTSAAELAARFINDRHLPDKAIDVIDEAGA 158 GV+ A+ A L+ R+I+ R LPDKAI V+D A A Sbjct: 392 FGVRVYDEAVVEAVRLSHRYISGRQLPDKAISVLDTACA 430 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 94/306 (30%), Positives = 166/306 (54%), Gaps = 18/306 (5%) Query: 177 IEEIISKIARIPPQSVNQDDRTKLQTIDRDLKNVVFGQDPAIEALASAIKMGVGKTE--- 233 + EI++ IP + +D+ + +D L+ V GQ A A+A ++ + Sbjct: 558 VAEIVAAWTGIPLGKMVKDEIQTVLDLDALLQERVLGQPQATSAVAQRVRTSRANLDDPG 617 Query: 234 ----VAKQLAXTGVGKTEVAKQLAFIL-GIE--LIRFDMSEYMERHAVSRLIGAPPGYVG 286 V + +GVGKTE A LA +L G E LI +MSEY E H VS L G+PPGYVG Sbjct: 618 KPKGVFLFVGPSGVGKTETALALADVLYGGERKLITINMSEYQEAHTVSGLKGSPPGYVG 677 Query: 287 FDQGGLLTEAITKKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVIIV 346 + +GG+LTEA+ + P++V+LLDE+EKAH D+ + QV D G + D GR+ DF+N II+ Sbjct: 678 YGEGGVLTEAVRRNPYSVVLLDEVEKAHADVLELFFQVFDKGVMDDAEGREIDFKNTIII 737 Query: 347 MTTNAGAESLQKRSIGFTDKKEAGDEMAD------IKRMFTPEFRNRLDSIISFRALDED 400 +T+N + + + + + ++ E D + + F P F RL ++ + + + Sbjct: 738 LTSNVASAQMMQACLNKSQEELPTPEELDTLIRPQLIKTFKPAFLGRL-KVVPYYPIADV 796 Query: 401 IILRVVDKFLMQLEEQLHEK-KVEAVFSDALRKFLSKKGFDPLMGARPMSRLIQDMIRKA 459 +++ ++ L ++ +++ E +VE + AL + + + + GAR + ++ + A Sbjct: 797 VLVEIIKLKLARIAKRVAENHRVEFSYDKALVEAVLGRCTEVDSGARNVDNILSGTLLPA 856 Query: 460 LADELL 465 +A+ +L Sbjct: 857 VAESVL 862 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,348,112 Number of extensions: 52298 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 7 Length of query: 509 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 420 Effective length of database: 1,198,455 Effective search space: 503351100 Effective search space used: 503351100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)