BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0082.0032.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbasp... 248 5e-67 gi|300313404|ref|YP_003777496.1| branched-chain amino acid ABC t... 124 1e-29 gi|300310684|ref|YP_003774776.1| amino acid ABC transporter ATPa... 101 1e-22 gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbasp... 100 2e-22 gi|300313301|ref|YP_003777393.1| branched-chain amino acid ABC t... 100 3e-22 >gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 640 Score = 248 bits (634), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 119/140 (85%), Positives = 130/140 (92%) Query: 1 LLKIIAGVSRLDDGLLVMQQGLKIAYVDQEPHFPAEMTIFNAVASGLGELPALLAEYESL 60 LLKIIAG S+LDDGLLVMQQ LKIAYVDQEPHFP EMT+F+AVASGLGELPALLAEYE++ Sbjct: 45 LLKIIAGRSKLDDGLLVMQQNLKIAYVDQEPHFPEEMTVFDAVASGLGELPALLAEYEAI 104 Query: 61 TGQFGGDNDEALMERMHDIQLILDAADAWSLNNKVETTLDKLNLKGEALIGELSGGMQKR 120 TGQFGGDND+AL+ERMH IQ +LDAADAWS+ NKVETTLDKLNL +A IGELSGGM+KR Sbjct: 105 TGQFGGDNDDALLERMHQIQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGMKKR 164 Query: 121 VALACGLVGAPDVLLLDEPT 140 VALACGLVG PDVLLLDEPT Sbjct: 165 VALACGLVGNPDVLLLDEPT 184 Score = 231 bits (589), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 146/222 (65%), Positives = 163/222 (73%), Gaps = 13/222 (5%) Query: 171 NGEANYMDQIDSVYALFPRLLERRKQAAGTLSGGERNRLLLARLFAKPANVLVLDEPTND 230 NG+ ++ S + P ER + TLSGGERNRLLLARLFAKPANVLVLDEPTND Sbjct: 414 NGQRKHVMTYLSDFLFAP---ERARSPVRTLSGGERNRLLLARLFAKPANVLVLDEPTND 470 Query: 231 LDIDTLELLEELLEDYEGTVFLVSHDRTFLDNVVTQVIASEGDGVWKEYVGGYSDWERVR 290 LDIDTLELLEELLE+YEGTVFLVSHDR FLDNVVTQVIASEG+GVW+EYVGGYSDWER R Sbjct: 471 LDIDTLELLEELLEEYEGTVFLVSHDRMFLDNVVTQVIASEGNGVWREYVGGYSDWERQR 530 Query: 291 --------ASTPASVKKSDGKSESKPAELPVAAPAVAKPKKLSYKEQRELEELPKLIAQL 342 + + KS+ K+ES A VA KKLSYKEQREL+ELPK IA L Sbjct: 531 PAQQAASSKAAAKAADKSEVKAESTMA--APVVAPVAAKKKLSYKEQRELDELPKRIAAL 588 Query: 343 EEEQAAISAKLADPDLYKAGGEEAGRLNARFAEIDGLLLENL 384 E EQ +SAKLADPDLYK G +EA +LN RFAEID LL +L Sbjct: 589 EAEQKELSAKLADPDLYKKGADEAVKLNQRFAEIDEELLVSL 630 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 35/75 (46%), Positives = 50/75 (66%) Query: 194 RKQAAGTLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIDTLELLEELLEDYEGTVFLV 253 R G LSGG + R+ LA +VL+LDEPTN LD ++ LE LL D++G++ + Sbjct: 150 RDARIGELSGGMKKRVALACGLVGNPDVLLLDEPTNHLDFGSILWLEGLLRDFKGSILFI 209 Query: 254 SHDRTFLDNVVTQVI 268 +HDR+FLDNV T++I Sbjct: 210 THDRSFLDNVSTRII 224 >gi|300313404|ref|YP_003777496.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 95/205 (46%), Positives = 114/205 (55%), Gaps = 23/205 (11%) Query: 31 PHFPAEMTIFNAVASGLGELPALLAEYESLTGQFGGDNDEALMERMHDIQLILDAADAWS 90 PH AE + A L E+ L A Y G+ D AL R I ++ Sbjct: 9 PHASAEPEVRATTAPVLLEVSGLSARY----GKVAALTDTALSLRAGQIVTVIGP----- 59 Query: 91 LNNKVETTLDKLNLKGEALIGELSGGM--QKRVALACGLVGAPDVLLLDEPTLGL-LVPE 147 N ++TL LN A++G L G R+A V +V + GL LVPE Sbjct: 60 -NGAGKSTL--LN----AMMGALPSGAAASGRIAFQGEDVSQMEVE--ERVARGLSLVPE 110 Query: 148 KRELFATMSVEDNLQLGAYKRKKNGEANYMDQIDSVYALFPRLLERRKQAAGTLSGGERN 207 KRELF TM+VEDNL LGAYKR +NGE+ Y+DQ D VY LFPRL ERRKQAAGTLSGGER Sbjct: 111 KRELFTTMTVEDNLLLGAYKRHRNGESGYLDQQDFVYTLFPRLKERRKQAAGTLSGGERQ 170 Query: 208 RLLLAR-LFAKPANVLVLDEPTNDL 231 L + R L AKP VL+LDEP+ L Sbjct: 171 MLAVGRALMAKP-QVLMLDEPSLGL 194 >gi|300310684|ref|YP_003774776.1| amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 51/86 (59%), Positives = 65/86 (75%), Gaps = 2/86 (2%) Query: 144 LVPEKRELFATMSVEDNLQLGAYKRKKNGEANYMDQIDSVYALFPRLLERRKQAAGTLSG 203 LVPEKRELFA M++EDNL LGA++R + G+ ++ ++ VY LFPRL ERR Q AGTLSG Sbjct: 83 LVPEKRELFAEMNIEDNLMLGAFQRYRTGQRDHAATLEEVYTLFPRLRERRSQQAGTLSG 142 Query: 204 GERNRLLLAR-LFAKPANVLVLDEPT 228 GER L + R L AKP +L+LDEP+ Sbjct: 143 GERQMLAVGRALMAKP-RLLMLDEPS 167 >gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 555 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 52/118 (44%), Positives = 74/118 (62%), Gaps = 7/118 (5%) Query: 194 RKQAAGTLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIDTLELLEELLEDYEGTVFLV 253 +++ G LSGGER RL LA+ + NVL+LDEP+NDLD++TL LE+ L ++ GTV ++ Sbjct: 438 QQKIVGNLSGGERGRLHLAKTLLQGGNVLLLDEPSNDLDVETLRALEDALLEFAGTVLVI 497 Query: 254 SHDRTFLDNVVTQVIASEGDGVWKEYVGGYSDWERVRASTPASVKKSDGKSESKPAEL 311 SHDR FLD + T +IA EGD + G Y ++E A KK G+ +KP + Sbjct: 498 SHDRWFLDRIATHIIAFEGDSQVTFFDGNYQEYE-------ADKKKRLGEEAAKPKRI 548 Score = 83.6 bits (205), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 56/143 (39%), Positives = 82/143 (57%), Gaps = 3/143 (2%) Query: 1 LLKIIAGVSRLDDGLLVMQQGLKIAYVDQEPHFPAEMTIFNAVASGLGELPALLAEYESL 60 LLKI+AG+ + +G + L I Y+ QEP AE T+ V S LGE+ A+ +++ Sbjct: 48 LLKIMAGIDKEIEGEAIPMPNLNIGYLPQEPQLDAEKTVRQEVESALGEVFEAQAKLDAV 107 Query: 61 TGQFGGDND--EALMERMHDIQLILDAADAWSLNNKVETTLDKLNL-KGEALIGELSGGM 117 + ++ +AL ++ I+ AAD SLN ++E D L L +A IG LSGG Sbjct: 108 YAAYAEEDADFDALATEQARLEAIIAAADGNSLNQQLEMAADALRLPPWDAKIGVLSGGE 167 Query: 118 QKRVALACGLVGAPDVLLLDEPT 140 ++RVAL L+ PD+LLLDEPT Sbjct: 168 KRRVALCKLLLSKPDMLLLDEPT 190 Score = 78.6 bits (192), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 39/90 (43%), Positives = 57/90 (63%), Gaps = 5/90 (5%) Query: 199 GTLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIDTLELLEELLEDYEGTVFLVSHDRT 258 G LSGGE+ R+ L +L ++L+LDEPTN LD ++++ LE+ L+ + GTV ++HDR Sbjct: 161 GVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQRFPGTVVAITHDRY 220 Query: 259 FLDNVVTQVIA-SEGDGV-WKEYVGGYSDW 286 FLDN ++ G G+ WK G YS W Sbjct: 221 FLDNAAEWILELDRGHGIPWK---GNYSSW 247 >gi|300313301|ref|YP_003777393.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 52/89 (58%), Positives = 67/89 (75%), Gaps = 2/89 (2%) Query: 144 LVPEKRELFATMSVEDNLQLGAYKRKKNGEANYMDQIDSVYALFPRLLERRKQAAGTLSG 203 LVPE+RELF+TMSVEDNL LG ++R + +A +DQ+ +V+ LFPRL ERR Q AGTLSG Sbjct: 83 LVPERRELFSTMSVEDNLLLGGFRRLRLRQAQPLDQLATVFDLFPRLKERRSQQAGTLSG 142 Query: 204 GERNRLLLAR-LFAKPANVLVLDEPTNDL 231 GER L + R + AKP +L+LDEP+ L Sbjct: 143 GERQMLAIGRAMMAKP-QLLMLDEPSLGL 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,305,599 Number of extensions: 49634 Number of successful extensions: 528 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 556 Number of HSP's successfully gapped: 27 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)