BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0082.0030.p (186 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl... 191 2e-50 gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 129 1e-31 >gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 822 Score = 191 bits (486), Expect = 2e-50, Method: Composition-based stats. Identities = 91/114 (79%), Positives = 105/114 (92%) Query: 4 LSLQRGFLNPNIAKFLAFSVLGLVIIGALIIAYALIFINPRLPSLDVITDYRPKIPLRIY 63 L+ QRGFLNPNIAKF+ S+LGL + GAL++ YALIFINPRLPSL++ITDYRPK+PLRI+ Sbjct: 14 LTRQRGFLNPNIAKFIGLSILGLGVAGALLVVYALIFINPRLPSLELITDYRPKVPLRIW 73 Query: 64 SADNVLLGEFGEEKRSLVKLDDIPANMKNAVLAIEDNRFYQHGGIDYIGIARAI 117 S D VLLGEFGEEKRSLVKLDDIPA+MKNAVLAIED RFY+HGGID+IGIARA+ Sbjct: 74 SEDKVLLGEFGEEKRSLVKLDDIPADMKNAVLAIEDYRFYEHGGIDWIGIARAL 127 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 129 bits (324), Expect = 1e-31, Method: Composition-based stats. Identities = 59/98 (60%), Positives = 75/98 (76%) Query: 19 LAFSVLGLVIIGALIIAYALIFINPRLPSLDVITDYRPKIPLRIYSADNVLLGEFGEEKR 78 L ++ GL ++ L + + + P+LP LD +TDYRPKIPLRI+SAD VL+GEFGEE+R Sbjct: 11 LVGTIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGVLIGEFGEERR 70 Query: 79 SLVKLDDIPANMKNAVLAIEDNRFYQHGGIDYIGIARA 116 +LV DIP MK AVLAIED+RFY+HGG+DY GIARA Sbjct: 71 NLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARA 108 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 395,269 Number of extensions: 10742 Number of successful extensions: 36 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 36 Number of HSP's successfully gapped: 2 Length of query: 186 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 106 Effective length of database: 1,241,070 Effective search space: 131553420 Effective search space used: 131553420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)