BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0082.0028.p (75 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl... 67 4e-13 >gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 822 Score = 66.6 bits (161), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 33/58 (56%), Positives = 39/58 (67%), Gaps = 13/58 (22%) Query: 4 IRFIDGEPYYENFTPGNGFVASLGVEGGGVVDALSNFFGWSKNPNSETQAAPSSGPAS 61 IRFIDGEPYY+NFTP NG V ++GV VVDA+SNFFGW+ PS PA+ Sbjct: 740 IRFIDGEPYYDNFTPNNGLVTNVGVT---VVDAISNFFGWT----------PSQAPAT 784 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.310 0.132 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 214,144 Number of extensions: 6628 Number of successful extensions: 19 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 18 Number of HSP's successfully gapped: 1 Length of query: 75 Length of database: 1,619,870 Length adjustment: 46 Effective length of query: 29 Effective length of database: 1,402,060 Effective search space: 40659740 Effective search space used: 40659740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 123 (52.0 bits)