BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0034.p (178 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 272 1e-74 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 178 3e-46 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 167 3e-43 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 142 2e-35 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 129 2e-31 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 272 bits (695), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 149/200 (74%), Positives = 158/200 (79%), Gaps = 34/200 (17%) Query: 1 LFFPTGNELTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSLTILLMAAGTTLI 60 LFFPTGN+LTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALS+TILLMA GTT+I Sbjct: 50 LFFPTGNDLTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMI 109 Query: 61 GIAPTYEQIGLFAPLIIVVARLLQG----------------------------------G 86 G+APTYE IGLFAPLIIVVARLLQG G Sbjct: 110 GLAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIG 169 Query: 87 VAVLLGAAVGTFVTSSLSPEALASWGWRLPFLLGIIIGPVGYYIRHHLDETPAFLEAKDK 146 VAVLLGAAVGTFVT+SL ALASWGWRLPFLLGIIIGPVGYYIRHHL+ETP FLE KDK Sbjct: 170 VAVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDK 229 Query: 147 TDSPLKEIIRNYPRETTASL 166 TDSPLKE+IRN+PRET+AS Sbjct: 230 TDSPLKEVIRNFPRETSASF 249 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 178 bits (451), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 96/203 (47%), Positives = 127/203 (62%), Gaps = 37/203 (18%) Query: 1 LFFPTGNELTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSLTILLMAAGTTLI 60 LFFP NE TSLL+A+ATFG+GFFMRPVGG+LLG+YADR GRKAA+ + I+LM +I Sbjct: 38 LFFPADNEYTSLLVALATFGIGFFMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAII 97 Query: 61 GIAPTYEQIGLFAPLIIVVARLLQG----------------------------------G 86 PTY IG+ AP++IVVAR+LQG Sbjct: 98 AFTPTYATIGIAAPIMIVVARMLQGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQC 157 Query: 87 VAVLLGAAVGTFVTSSLSPEALASWGWRLPFLLGIIIGPVGYYIRHHLDETPAFLEAKDK 146 +AV GA +G VT +LSPEAL SWGWRLPF+LG++IGPVG +IR H+ ET FLE+ +K Sbjct: 158 LAVFCGAGMGALVTRNLSPEALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANK 217 Query: 147 TD-SPLK--EIIRNYPRETTASL 166 + +P+K ++++ R S+ Sbjct: 218 PNAAPIKLLDVVKTNLRGVLVSM 240 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 167 bits (424), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 83/185 (44%), Positives = 113/185 (61%), Gaps = 33/185 (17%) Query: 2 FFPTGNELTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSLTILLMAAGTTLIG 61 FFPTG++ SLLL ATFGV FFMRP+G ++LG Y+DR GRKAAL L+I+LM GT +I Sbjct: 49 FFPTGDDSVSLLLTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIA 108 Query: 62 IAPTYEQIGLFAPLIIVVARLLQG---------------------------------GVA 88 P+Y IGL AP I + +++QG G++ Sbjct: 109 FMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGIS 168 Query: 89 VLLGAAVGTFVTSSLSPEALASWGWRLPFLLGIIIGPVGYYIRHHLDETPAFLEAKDKTD 148 +LL A G + + L+PE LASWGWR+PF+ G++I P G YIR HLDE P F E+ +KT+ Sbjct: 169 LLLAALFGAVLNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTN 228 Query: 149 SPLKE 153 +PL++ Sbjct: 229 APLRD 233 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 142 bits (357), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 75/193 (38%), Positives = 106/193 (54%), Gaps = 36/193 (18%) Query: 2 FFPTGNELTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSLTILLMAAGTTLIG 61 FFP+ NE SL+L TFG GF MRP+G I+LG Y DRVGR+ L +T+ LMA GT LI Sbjct: 42 FFPSDNEFASLMLTFMTFGAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIA 101 Query: 62 IAPTYEQIGLFAPLIIVVARLLQG----------------------------------GV 87 P+Y IG APL++++ RLLQG V Sbjct: 102 FVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQV 161 Query: 88 AVLLGAAVGTFVTSSLSPEALASWGWRLPFLLGIIIGPVGYYIRHHLDETPAFLEAKDK- 146 A+++ AA+G +++SL+P ++ WGWR+PF +G +I PV + IR L ET F+ K + Sbjct: 162 AIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRP 221 Query: 147 -TDSPLKEIIRNY 158 T + +I N+ Sbjct: 222 STGQIFRSMIENW 234 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 129 bits (323), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 73/177 (41%), Positives = 95/177 (53%), Gaps = 34/177 (19%) Query: 2 FFPTGNELTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSLTILLMAAGTTLIG 61 FFP+ NE SL+L TFG GF MRP+G I+LG Y DRVGR+ L +T+ +MA GT LI Sbjct: 44 FFPSDNEFVSLILTFGTFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIA 103 Query: 62 IAPTYEQIGLFAPLIIVVARLLQ--------GGVAVLLG--------------------- 92 P Y IG+ AP++++ RLLQ GGV+V L Sbjct: 104 FVPGYATIGVAAPILVLTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQA 163 Query: 93 -----AAVGTFVTSSLSPEALASWGWRLPFLLGIIIGPVGYYIRHHLDETPAFLEAK 144 A +G V+ SLSP ++ WGWR+PF +G +I P + IR L ET FL K Sbjct: 164 AIMASALIGYLVSQSLSPVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARK 220 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 530,661 Number of extensions: 20480 Number of successful extensions: 168 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 15 Length of query: 178 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 99 Effective length of database: 1,245,805 Effective search space: 123334695 Effective search space used: 123334695 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)