BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0033.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 260 6e-71 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 115 2e-27 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 115 3e-27 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 100 1e-22 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 94 5e-21 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 260 bits (664), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 134/152 (88%), Positives = 141/152 (92%) Query: 9 SFSMVILWTVCTYVLLFYMPTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRLADRYG 68 SFSMVILWTVCTYVLLFYMPTYS+KVLKLPQ+TGFIAGMVGGLCIMV AP+VGRLADR G Sbjct: 248 SFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIG 307 Query: 69 RRGLLSGAALLILVLAYPMFAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPA 128 RR LLSG+A LIL+LAYPMFAYIN AP L +LL+FQLVFG LIA YTGPILAAFSELFPA Sbjct: 308 RRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPA 367 Query: 129 KVLSTGLSVAYNLAVTIFGGFASFIITGLIAA 160 KVLSTGLSVAYNLAVTIFGGFASF IT LIAA Sbjct: 368 KVLSTGLSVAYNLAVTIFGGFASFFITWLIAA 399 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 115 bits (289), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 67/149 (44%), Positives = 91/149 (61%) Query: 9 SFSMVILWTVCTYVLLFYMPTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRLADRYG 68 S VI TV YV+L MPT++ K L LP F+ M+ + P G L+DR G Sbjct: 239 SMGQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLG 298 Query: 69 RRGLLSGAALLILVLAYPMFAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPA 128 RR +L ++L L++ YP+F +++ AP L LLV Q++ I+I GP+ A SE FP Sbjct: 299 RRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPT 358 Query: 129 KVLSTGLSVAYNLAVTIFGGFASFIITGL 157 +V STGL+VAYNLA +FGGFA FI+T L Sbjct: 359 RVRSTGLAVAYNLAAMLFGGFAPFIVTWL 387 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 115 bits (287), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 65/146 (44%), Positives = 95/146 (65%), Gaps = 1/146 (0%) Query: 13 VILWTVCTYVLLFYMPTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRLADRYGRRGL 72 VI+ TV Y+ L Y+PTY++K L LP + F A V GL + + +P+VG L+D+ GR Sbjct: 249 VIMATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPF 307 Query: 73 LSGAALLILVLAYPMFAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLS 132 + ++ L +VL YPMF ++ ++PG LL+ Q V G+L+ Y + A +++FP Sbjct: 308 MITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRG 367 Query: 133 TGLSVAYNLAVTIFGGFASFIITGLI 158 TG+S+AYN+AVTIFGGFA IIT LI Sbjct: 368 TGMSLAYNIAVTIFGGFAGLIITWLI 393 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 99.8 bits (247), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 55/147 (37%), Positives = 83/147 (56%) Query: 12 MVILWTVCTYVLLFYMPTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRLADRYGRRG 71 +V + TV Y++ Y PT+ VLKL I + GL ++ P++G L+DR GRR Sbjct: 243 LVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRP 302 Query: 72 LLSGAALLILVLAYPMFAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVL 131 LL +L ++ AYP ++ H +L+ +L L A+Y G ++ A +E+ PA V Sbjct: 303 LLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVR 362 Query: 132 STGLSVAYNLAVTIFGGFASFIITGLI 158 + G S+AY+LA +FGGF I TGLI Sbjct: 363 TAGFSLAYSLATAVFGGFTPAIATGLI 389 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 94.4 bits (233), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 55/142 (38%), Positives = 79/142 (55%) Query: 17 TVCTYVLLFYMPTYSIKVLKLPQSTGFIAGMVGGLCIMVFAPVVGRLADRYGRRGLLSGA 76 TV Y++ Y PT+ VLKL S I GL + P++G L+DR GRR +L Sbjct: 250 TVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVLVAF 309 Query: 77 ALLILVLAYPMFAYINHAPGLGSLLVFQLVFGILIATYTGPILAAFSELFPAKVLSTGLS 136 LL L+ AYP+ +++ +LL+ +L L A+Y G + A +E+ PA V + G S Sbjct: 310 TLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVGFS 369 Query: 137 VAYNLAVTIFGGFASFIITGLI 158 +AY+LA IFGGF + T LI Sbjct: 370 LAYSLATAIFGGFTPLVSTWLI 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.332 0.148 0.460 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 469,278 Number of extensions: 16384 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 8 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 127 (53.5 bits)