BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0032.p (214 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herba... 276 8e-76 >gi|300309541|ref|YP_003773633.1| aspartyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 599 Score = 276 bits (707), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 127/137 (92%), Positives = 134/137 (97%) Query: 5 AIGALRVKIGHSDFGKKNGLFDDVWRPLWVVDFPMFEYDEDNQRWVALHHPFTSPKDGHE 64 AIGALRVKIGHS+FGKKNGLFDD WRPLWVVDFPMFEYDED QRWVALHHPFTSPKDGHE Sbjct: 407 AIGALRVKIGHSEFGKKNGLFDDAWRPLWVVDFPMFEYDEDGQRWVALHHPFTSPKDGHE 466 Query: 65 DFISTNPAAAIAKAYDLVLNGWEMGGGSVRIHRAEVQSKVFRALKINDEEARLKFGFLLD 124 DFISTNP AAIAKAYD+VLNGWE+GGGSVRIHRA+VQSKVFRALKI+DEEA+LKFGFLLD Sbjct: 467 DFISTNPGAAIAKAYDMVLNGWELGGGSVRIHRADVQSKVFRALKIDDEEAKLKFGFLLD 526 Query: 125 ALQYGAPPHGGIAFGLE 141 ALQYGAPPHGGIAFGL+ Sbjct: 527 ALQYGAPPHGGIAFGLD 543 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 693,616 Number of extensions: 28294 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 85 Number of HSP's successfully gapped: 1 Length of query: 214 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 133 Effective length of database: 1,236,335 Effective search space: 164432555 Effective search space used: 164432555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)