BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0081.0021.p
         (379 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309537|ref|YP_003773629.1| glucoamylase [Herbaspirillum se...   457   e-130

>gi|300309537|ref|YP_003773629.1| glucoamylase [Herbaspirillum
           seropedicae SmR1]
          Length = 595

 Score =  457 bits (1177), Expect = e-130,   Method: Compositional matrix adjust.
 Identities = 239/369 (64%), Positives = 266/369 (72%), Gaps = 63/369 (17%)

Query: 24  PSTSSLDLGVIGNCSYSALIDKLGRVVWCCLPRFDGDPVFNALLDPGESGSLWSFELENF 83
           PSTSSL+LGVIGNC++SAL+DK+GR+VWCCLPRFDGDPVFNALLDP ++G+LW+F+LENF
Sbjct: 2   PSTSSLELGVIGNCAFSALVDKMGRIVWCCLPRFDGDPVFNALLDPTDNGALWAFQLENF 61

Query: 84  S------------------------------XPRFD----WGREVPEITR-----GSNHV 104
           S                               PRF     + R +  + R     G+  +
Sbjct: 62  SHSEQWYEPNTAVLRTRLYDTLGQGIEITDLAPRFQSRGRFFRPLQLVRRIHPLSGAPRM 121

Query: 105 RYV-------GSEMTLRLNTDAPISYLLDETAFVISR--PFNFL---------------L 140
           R V       G E  L+      I Y+  E    ++   P +++               L
Sbjct: 122 RVVLRPKFDWGREEPLQTRGSNHIRYVGQEQTLRLNTDAPISYIVGETAFVVSRPYNFIL 181

Query: 141 GPDETLTGGIGDTARSFEQETITYWRNWSRSLATPLEWQAAVIRAAITLKMSVYEDTGAI 200
           GPDETL GGI DTAR FEQET  YWRNWSR+LATPLEWQ AVIRAAITLK+SVYEDTGAI
Sbjct: 182 GPDETLHGGINDTARDFEQETTAYWRNWSRALATPLEWQDAVIRAAITLKLSVYEDTGAI 241

Query: 201 IAAMTTSVPEAPGSARNWDYRYCWLRDAFFVVRALNSISELGTMEDYLRWLTNVVVRSAG 260
           IAAMTTSVPEAPGS RNWDYRYCWLRDAFFVVRALNS+SELGTMEDYLRWLTNVVVRS G
Sbjct: 242 IAAMTTSVPEAPGSQRNWDYRYCWLRDAFFVVRALNSLSELGTMEDYLRWLTNVVVRSGG 301

Query: 261 GHVQPLYGIGLEEALPEQILDHLPGYRHHAPVRVGNQAYEHFQHDVYGNIVLGAAQAFHD 320
           GHVQPLYGIGLEEALPEQILDHLPGY+HH PVRVGNQAYEHFQHDVYGNIVLGAAQAFHD
Sbjct: 302 GHVQPLYGIGLEEALPEQILDHLPGYQHHQPVRVGNQAYEHFQHDVYGNIVLGAAQAFHD 361

Query: 321 ARLHHRAGG 329
            RLHHRAG 
Sbjct: 362 MRLHHRAGA 370


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.136    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,237,407
Number of extensions: 52234
Number of successful extensions: 127
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 127
Number of HSP's successfully gapped: 2
Length of query: 379
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 292
Effective length of database: 1,207,925
Effective search space: 352714100
Effective search space used: 352714100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)