BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0081.0020.p
         (481 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309535|ref|YP_003773627.1| Na+/proline symporter protein [...   847   0.0  

>gi|300309535|ref|YP_003773627.1| Na+/proline symporter protein
           [Herbaspirillum seropedicae SmR1]
          Length = 485

 Score =  847 bits (2187), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 416/469 (88%), Positives = 448/469 (95%)

Query: 1   MNALIWFVILYWVISVGIGLYASRYVKSGKDFAVAGRALPMSVVTATVFATWFGSEAVLG 60
           MNAL+WFV+LYWVISVGIGLYA+R+VKS KD+AVAGR+LPMSVVTATVFATWFGSEAVLG
Sbjct: 1   MNALLWFVVLYWVISVGIGLYATRFVKSSKDYAVAGRSLPMSVVTATVFATWFGSEAVLG 60

Query: 61  VSSTFLKEGLGGIIADPFGSSLCLILVGVFFARPLYRMNLLTIGDYYKKRFGRAAEMLVT 120
           VSSTFLKEGLGGI+ADPFGSSLCLILVG+FFA+ LYRMNLLTIGDYYKKRFGRAAEMLVT
Sbjct: 61  VSSTFLKEGLGGIVADPFGSSLCLILVGLFFAKRLYRMNLLTIGDYYKKRFGRAAEMLVT 120

Query: 121 ICIVISYLGWVAAQIKALGLVFNVVSGGYITMQAGMIIGAASVLVYTLMGGMWAVAITDF 180
           ICIVISYLGWVAAQIKALGLVFNVVSGGYI M  GMIIGA SVL+YTLMGGMWAVAITDF
Sbjct: 121 ICIVISYLGWVAAQIKALGLVFNVVSGGYIPMHWGMIIGAGSVLIYTLMGGMWAVAITDF 180

Query: 181 LQMIIVVVGMLYIGWEVSGMVGGPGVVIQHAADAGKFNFWPAPSLREVMWFFAAWITMML 240
           LQMIIVVVGMLYIG+EVSGMVGGPGVVI HAA+AGKFNFWPA  L++V+WFFAAWITMML
Sbjct: 181 LQMIIVVVGMLYIGYEVSGMVGGPGVVIAHAAEAGKFNFWPAAELKDVLWFFAAWITMML 240

Query: 241 GSIPQQDVFQRVMSSKTEKIARNASILGGVIYFGFVFIPIFLAYSATLIDPAMVSRLLET 300
           GSIPQQDVFQRVMSSK E +ARNAS+LGGVIYF FVFIPIFLAYSATLIDP MVSRLLET
Sbjct: 241 GSIPQQDVFQRVMSSKNENVARNASVLGGVIYFFFVFIPIFLAYSATLIDPEMVSRLLET 300

Query: 301 DSQLVLPTLIMEKMPVFAQVMFFGALLSAIKSCASATLLAPSVTFTENVIKEIRPWKNDK 360
           DSQLVLPTLIMEKMPVFAQVMFFGALLSAI+SCASATLLAPSVTFTENV+KEIRPWK+D+
Sbjct: 301 DSQLVLPTLIMEKMPVFAQVMFFGALLSAIRSCASATLLAPSVTFTENVLKEIRPWKSDR 360

Query: 361 EFLFSMRVVVLLFTVAVTSLALNSQSSIYQMVENAYKVTLVAAFVPLAFGVYWKRATRQG 420
           EFLFSMR VVL+FT+ VTSLALNSQSSIY+MVENAYKVTLVAAFVPLAFGVYW RA+RQG
Sbjct: 361 EFLFSMRAVVLVFTIGVTSLALNSQSSIYEMVENAYKVTLVAAFVPLAFGVYWPRASRQG 420

Query: 421 ALAAMILGLATWITLEYVAPEGLWPPQLAGLLMSVGGMVFGSLLPQLVA 469
           AL AM+LGL +WITLEYVAP+G WPPQLAGLL S+GGMV GSL+PQ++A
Sbjct: 421 ALMAMVLGLVSWITLEYVAPDGFWPPQLAGLLFSIGGMVVGSLMPQVLA 469


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.140    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,509,424
Number of extensions: 65106
Number of successful extensions: 318
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 317
Number of HSP's successfully gapped: 2
Length of query: 481
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 392
Effective length of database: 1,198,455
Effective search space: 469794360
Effective search space used: 469794360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)