BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0010.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 678 0.0 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 117 2e-27 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 111 8e-26 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 85 9e-18 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 65 7e-12 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 678 bits (1749), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/383 (85%), Positives = 351/383 (91%) Query: 1 MAYLLALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQ 60 MAYLLALDQGTSSSRSIIFD++GAIVATAQQEF QIFPQPGWVEHDPMDLW+SQLQT ++ Sbjct: 1 MAYLLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQK 60 Query: 61 VLAQAGIKAADLAGLGITNQRETTVVWDRASGKPVHNAIVWQDRRTEAICAGLRERGLTD 120 VLAQ+GIKA++L LGITNQRETTVVW+RA+GKPV+NA+VWQDRR E +CA LRERGL + Sbjct: 61 VLAQSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGE 120 Query: 121 AIQAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTAGRLHVTD 180 AIQ KTGLVLDPYFSGTKL WILD V GLR RA RGEL FGTVD+WL+WNLT GRLHV+D Sbjct: 121 AIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 Query: 181 VSNASRTMLWNIHTGSWDEELLEWLDVPRSLLPEVHPSAHLYGRTDAGHLGAAVMIGGIA 240 VSNASRTMLWNIHTG+WDEELL+WL +P ++LP+V PSAHLYG DA HLGAAV IGGIA Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEIDAQHLGAAVKIGGIA 240 Query: 241 GDQQSALFGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGERKQYALE 300 GDQQSALFGQAC +PGMAKNTYGTGCFMLLHTGDQCA S NGLISTAACQV E+KQYALE Sbjct: 241 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQYALE 300 Query: 301 GSVFIGGAVVQWLRDGLKAIKNSTDVEELAASAPDSGGVVFVPSFTGLGAPYWNPSAKGA 360 GSVFIGGAVVQWLRDGLKAIK STDVE LA S DSGGVVFVPSFTGLGAPYWNPSAKGA Sbjct: 301 GSVFIGGAVVQWLRDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNPSAKGA 360 Query: 361 IVGLSRGSTVAHIARAALESIAF 383 IVGLSRGSTVAHIARAALESIAF Sbjct: 361 IVGLSRGSTVAHIARAALESIAF 383 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 105/401 (26%), Positives = 179/401 (44%), Gaps = 23/401 (5%) Query: 1 MAYLLALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQ 60 M+YL +D GTS + ++ DE ++A+ R P P W E P WN+ L Sbjct: 1 MSYL-GIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAA 59 Query: 61 VLAQAGIKAADLAGLGITNQRETTVVWDRASGKPVHNAIVWQDRRTEAICAGLRERGLTD 120 + QA L G+G++ Q + DR +G + AI+W D R A C L E + D Sbjct: 60 LRTQAPQAFQALRGIGLSGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVEL-EALVPD 117 Query: 121 AIQAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTAGRLHVTD 180 A+ A TG P F+ KL W+ P + + L +L W LT V++ Sbjct: 118 AV-AITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLP----KDFLGWKLTGE--FVSE 170 Query: 181 VSNASRTMLWNIHTGSWDEELLEWLDVPRSLLPEVHPSAHLYG------RTDAGHLGAAV 234 +S+A+ T+ ++ W E +L + RS +P + + + G R + G G V Sbjct: 171 MSDAAGTLWLDVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVV 230 Query: 235 MIGGIAGDQQSALFGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGER 294 + GG AGD ++ G + G A + G+ + T ++ G+ + C G+ Sbjct: 231 VAGG-AGDNAASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQW 289 Query: 295 KQYALEGSVFIGGAVVQWLRDGLKA--IKNSTDVEELAASAPDSGGVVFVPSFTGLGAPY 352 Q ++ + A + WL G+ A + E ++ + +F+P G P+ Sbjct: 290 HQMSV---ILSAAASLHWL-SGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPH 345 Query: 353 WNPSAKGAIVGLSRGSTVAHIARAALESIAFXIATCRSCLR 393 + +AKG + G++ AH+A A +E +AF +A + L+ Sbjct: 346 NDAAAKGVLFGMTPAHESAHLAYAVMEGVAFAMADGYAALQ 386 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 111 bits (278), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 105/409 (25%), Positives = 168/409 (41%), Gaps = 31/409 (7%) Query: 3 YLLALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQVL 62 Y+L +D GT+S++S++F G +VA +E+ + +PG E DP + + L + + Sbjct: 9 YMLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAV 68 Query: 63 AQAGIKAADLAGLGITNQRETTVVWDRASGKPVHNAIVWQDRRTEAICAGLRERGLTDAI 122 A ++A L + + + D A + + N+I W D R ++ +AI Sbjct: 69 KAAKAAPQEIALLSFSAAMHSVIALD-AENRLLSNSITWGDIRASVWAERIKHEHDGNAI 127 Query: 123 QAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTAGRLHVTDVS 182 +TG + P KL W+ P + RA R F + +L++ L V D S Sbjct: 128 YRRTGTPVHPMSPLCKLMWMRHEKPDVFHRAAR----FVGIKEYLLYQLFGQW--VVDHS 181 Query: 183 NASRTMLWNIHTGSWDEELLEWLDVPRSLLPEVHPSAH---LYGRTDAGHLGAAVMIGGI 239 AS T ++N+ +WD+ L L V LP P+ H A LG +V I Sbjct: 182 IASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFI 241 Query: 240 AGDQQSAL--FGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGERKQY 297 G L G + G T GT M + + L C K + Sbjct: 242 IGANDGVLSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRLF----CYALTEKHW 297 Query: 298 ALEGSVFIGGAVVQWLRD-------------GLKAIKNSTDVEELAASAPDSGGVVFVPS 344 + G V GG + +W+RD G+ + T + E P + G++F P Sbjct: 298 VVGGPVNNGGNIFRWVRDELATAEAAAAREEGVDPYEALTRIAE--KVRPGAEGLLFHPF 355 Query: 345 FTGLGAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFXIATCRSCLR 393 G AP WN +G+ GL+ H+ RAALE + F + + L Sbjct: 356 MAGERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALE 404 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 85.1 bits (209), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 102/401 (25%), Positives = 173/401 (43%), Gaps = 29/401 (7%) Query: 5 LALDQGTSSSRSIIFDETGAIVATAQQEFRQIFPQPGWVEHDPMDLWNSQLQTCRQVLAQ 64 L +D GTS ++++ D G++VA A P P W E P D W + T ++ Q Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 65 AGI-KAADLAGLGITNQRETTVVWDRASGKPVHNAIVWQDRRTEAICAGLRERGLTDAIQ 123 G + + +G++ Q V+ D + + AI+W D R+ CA L R + Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDERD-EVLRPAILWSDSRSAPECAELESR--APRLH 119 Query: 124 AKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTV---DAWLIWNLTAGRLHVTD 180 G + P F+ KL W+ P L R TV WL +T GR V+D Sbjct: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRLKMT-GR-KVSD 170 Query: 181 VSNASRTMLWNIHTGSWDEELLEWLDVPRSLLPEVHPSAHLYGR-----TDAGHLGAAVM 235 S+A+ T+ ++ +W +ELL + R +P + + + G A L + V+ Sbjct: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVI 230 Query: 236 IGGIAGDQQSALFGQACFQPGMAKNTYGTGCFMLLHTGDQCAQSRNGLISTAACQVGERK 295 + G AGD ++ G +PG + GT + + D+ + G A C ++ Sbjct: 231 VAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 Query: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKNS--TDVEELAASAPDSGGVVFVPSFTGLGAPY 352 + + SV + A ++W + S ++E+L A ++ +F+P +G P+ Sbjct: 289 WHQM--SVMLSAASCLRWFCRLCSVDEKSLLAEIEQLDEQACNN-APLFLPYLSGERTPH 345 Query: 353 WNPSAKGAIVGLSRGSTVAHIARAALESIAFXIATCRSCLR 393 +P A G GL+ A + A LE +AF + LR Sbjct: 346 NDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDALR 386 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 57/205 (27%), Positives = 91/205 (44%), Gaps = 21/205 (10%) Query: 3 YLLALDQGTSSSRSIIFDETGAIVATAQQE---FRQIFPQPGWVEHDPMDLWNSQLQTCR 59 YL+ +D GT S+R+ +FD G ++A+A+ + FR + VE +W + Q R Sbjct: 4 YLIGIDVGTGSARAGVFDPQGQLLASARHDIDLFRD--ERRARVEQSSAQIWEAVCQAVR 61 Query: 60 QVLAQAGIKAADLAGLGITNQRETTVVWDRAS----GKPVHNAIVWQDRRTEAICAGLRE 115 + ++GI A +AG+G+ V A G P + IVW D R A+ R Sbjct: 62 AAVTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHR--ALEQAQRI 119 Query: 116 RGLTDAIQAKTGLVLDPYFSGTKLHWILDSVPGLRERARRGELAFGTVDAWLIWNLTAGR 175 + A+ + G V+ P KL W+ +++P + A F + +L W T G Sbjct: 120 NRMGHAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAH----FFDLTDFLTWKAT-GS 174 Query: 176 LHVTDVSNASRTMLWNI--HTGSWD 198 L S+ + T W H WD Sbjct: 175 LQ---RSSCTVTCKWTYLAHEAGWD 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,390,081 Number of extensions: 61350 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 5 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)