BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0080.0003.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 461 e-131 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 230 1e-61 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 228 4e-61 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 228 5e-61 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 67 2e-12 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 461 bits (1186), Expect = e-131, Method: Compositional matrix adjust. Identities = 235/256 (91%), Positives = 243/256 (94%), Gaps = 5/256 (1%) Query: 1 MNLEIAT---EQXP--GVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKEKGGIAMYSG 55 M +EI + + P GVTRAREWLDRDKVEAIADLTNSSVALAVQKLIK+KGGIAMYSG Sbjct: 75 MKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKGGIAMYSG 134 Query: 56 PATGALTTAECASNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASD 115 PATGALTTAECA NGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASD Sbjct: 135 PATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASD 194 Query: 116 VVKANGGTVLGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGG 175 VVKANGGTV+GSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGG Sbjct: 195 VVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGG 254 Query: 176 KDQRLVALLVFLSDVHALGLDAAQGLVYTDGFYWDYDEQTRGFSKRFEAQFKNNKPTMVQ 235 KDQRLVALLVFLSDVHALGL+AAQGLVYTDGFYWDYDEQTR FSKRFE+QFKN KPTMVQ Sbjct: 255 KDQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSKRFESQFKNIKPTMVQ 314 Query: 236 AGVYSSVYHFLKARAS 251 AGVYSSVY FLKARA+ Sbjct: 315 AGVYSSVYQFLKARAA 330 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 230 bits (586), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 115/234 (49%), Positives = 159/234 (67%), Gaps = 2/234 (0%) Query: 14 TRAREWLDRDKVEAIADLTNSSVALAVQKLIKEKGGIAMYSGPATGALTTAECASNGFHW 73 ++AREW D+ V+ + TNS ALA K+ EK + M G + LT EC+ H+ Sbjct: 87 SKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTVHY 146 Query: 74 MFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASDVVKANGGTVLGSVRHPLN 133 +DT + A G +A+ K G KSW+F+T DYAFGHSLE D ++VVKA+GGTV+G+V+HPL Sbjct: 147 AYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHPLG 206 Query: 134 ASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHAL 193 ASDFSSFLLQAQ+SKAQ+IGLA+ G D +N++K A F + K +L LLVF++DVH+L Sbjct: 207 ASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGI-TKTAKLAGLLVFINDVHSL 265 Query: 194 GLDAAQGLVYTDGFYWDYDEQTRGFSKRFEAQFKNNKPTMVQAGVYSSVYHFLK 247 L +GL TD +YWD +E+TR F+KR+ A + P+ +QA YS+ +LK Sbjct: 266 TLKNTEGLYLTDSWYWDANEETRAFAKRYFA-VRKKMPSSLQAADYSAALQYLK 318 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 228 bits (581), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 116/235 (49%), Positives = 158/235 (67%), Gaps = 2/235 (0%) Query: 14 TRAREWLDRDKVEAIADLTNSSVALAVQKLIKEKGGIAMYSGPATGALTTAECASNGFHW 73 TRAREW D ++ + NS+ AL++ K++ EK + + G T LT EC H+ Sbjct: 89 TRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINIGAGTARLTNEECTPYTVHY 148 Query: 74 MFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASDVVKANGGTVLGSVRHPLN 133 +DT + A G A+A+ K G KSW+F+ DYAFGHSL D S VVKA+GGTV+GS++HP Sbjct: 149 EYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLANDTSAVVKASGGTVVGSIKHPPQ 208 Query: 134 ASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHAL 193 +SD SSFLLQAQASKAQ++GLA+ G D VN+IK A+ F V K +LV LL+ ++D+HAL Sbjct: 209 SSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGV-TKTMKLVGLLMVINDIHAL 267 Query: 194 GLDAAQGLVYTDGFYWDYDEQTRGFSKRFEAQFKNNKPTMVQAGVYSSVYHFLKA 248 GL A+GL+ TD +YWD DE +R F++RF + K P+ QA YS+ +LKA Sbjct: 268 GLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMK-KMPSTHQAAAYSATTTYLKA 321 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 228 bits (581), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 117/234 (50%), Positives = 157/234 (67%), Gaps = 2/234 (0%) Query: 15 RAREWLDRDKVEAIADLTNSSVALAVQKLIKEKGGIAMYSGPATGALTTAECASNGFHWM 74 +AREW DRD V+ + NS+ +LA+ K+ EK + + G T T EC + + Sbjct: 90 KAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTIQYA 149 Query: 75 FDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASDVVKANGGTVLGSVRHPLNA 134 +DT + A G AA+T+ G KSWYF+T DYAFG SLEKD S+VVK+NGG V+G+ R PL Sbjct: 150 YDTTALARGTGAAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLAT 209 Query: 135 SDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALG 194 SDFSSFLLQAQ+SKAQ++GLA G DV+NAIK A F V K +L LL+F++D H+LG Sbjct: 210 SDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGV-NKTMKLAGLLIFINDTHSLG 268 Query: 195 LDAAQGLVYTDGFYWDYDEQTRGFSKRFEAQFKNNKPTMVQAGVYSSVYHFLKA 248 L QG+ TDG+YWD ++QTR ++ R+ + K P+M QAG S+V +LKA Sbjct: 269 LSLTQGMYLTDGWYWDLNDQTRAWANRYFMKMK-KMPSMFQAGDASAVGQYLKA 321 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 63/220 (28%), Positives = 92/220 (41%), Gaps = 9/220 (4%) Query: 34 SSVALAVQKLIKEKGGIAMYSGPATGALTTAECASNGFHWMFDTYSQAAGAAAAL-TKLG 92 S ALA+ K + G + + + A +T ++C F W T+ L K+ Sbjct: 104 SGEALAMGKEADKAGAVFITTAGAD-EITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMP 162 Query: 93 N-KSWYFVTVDYAFGHSLEKDASDVVKANGGTVLGSVRHPLNASDFSSFLLQAQASKAQV 151 N K WY +T Y FG L A ++ K G +G+ H L +FS +L A A+K V Sbjct: 163 NAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDV 222 Query: 152 IGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLDAAQGLVYTDGFYWDY 211 + L + G+ + ++QA SF + L+A L LG D G VY YW Sbjct: 223 LLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETLGADICDG-VYFGAQYWHA 281 Query: 212 DEQTRGFSKRFEAQFKNN---KPTMVQAGVYSSVYHFLKA 248 + F+K F +N P AG Y L A Sbjct: 282 IDAP--FNKEFVKLVNDNLKIDPNYSLAGSYICTKIILDA 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 908,188 Number of extensions: 35968 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 5 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)