BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0080.0002.p (744 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 868 0.0 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 422 e-119 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 414 e-116 gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 268 2e-72 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 213 4e-56 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 868 bits (2244), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/443 (94%), Positives = 435/443 (98%) Query: 1 MSQANIPNVPLYINGEHVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKEAFK 60 MSQANIPNVPLY+NGE VQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKE FK Sbjct: 1 MSQANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFK 60 Query: 61 TWRNTSLAQRMRIMLKFQQLLRDNIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 TWRNTSLAQRMRIMLKFQQLLR+NIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI Sbjct: 61 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 Query: 121 TSLQLGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 TSLQLGELAENVAGGVDVYT++QP+GVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS Sbjct: 121 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 Query: 181 EQDPTSSLFLVELANKAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTNVGTHVYR 240 EQDPTSSLFLVELAN+AGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGST+VGTH+YR Sbjct: 181 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR 240 Query: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKE 300 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVG+T+E Sbjct: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 Query: 301 WIPEIVERSKAMKVGPGSDRKADVGPLVSKAAKERVERLIGVGVEQGAKLLLDGRNCKVP 360 WIPEIVERSKAMKVGPG+DRKADVGPLVSKAAKERVERLI GVEQGAKLLLDGRNCKV Sbjct: 301 WIPEIVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVA 360 Query: 361 GNESGNFVGPTVFTGVKAGMDIYEQEIFGPAMCIVEVDTLDDAIAFINANPNGNGTSIFT 420 G+E+GNFVGPTVFTGVK MDIY QEIFGPAMCIVE+DTLD+AIAFINANPNGNGTSIFT Sbjct: 361 GSENGNFVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFT 420 Query: 421 SSGWAARKFQNEIDVGQVGINVP 443 SSG+AARKFQNEIDVGQVGINVP Sbjct: 421 SSGYAARKFQNEIDVGQVGINVP 443 Score = 181 bits (458), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 89/118 (75%), Positives = 96/118 (81%), Gaps = 5/118 (4%) Query: 576 LGLAVENALATGSSGPGPSRTRWL-----PAAAPPGVLNVVHGGPDVANMICDHPDIKAV 630 + +A N S P+ + +L A PPGVLNVVHGGPDVANMICDHPDIKAV Sbjct: 167 IAVACGNTFVLKPSEQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAV 226 Query: 631 SFIGSTNVGTHVYRRASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQ 688 SFIGST+VGTH+YRRASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQ Sbjct: 227 SFIGSTHVGTHIYRRASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQ 284 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 422 bits (1084), Expect = e-119, Method: Compositional matrix adjust. Identities = 204/433 (47%), Positives = 285/433 (65%), Gaps = 1/433 (0%) Query: 12 YINGEHVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKEAFKTWRNTSLAQRM 71 YI G+ V + S + DV NPA VA+V T +EVD AV AF W NT R Sbjct: 7 YIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPLTRA 66 Query: 72 RIMLKFQQLLRDNIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 R++ +F L++ A +I REHGKT DA+GEV RG+E+VE A I + GE + Sbjct: 67 RVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQ 126 Query: 132 VAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 191 +A G+D +++ Q +GV AGIT FNFP M+P +MFPIA+ACGN FVLKPSE+DP++SL + Sbjct: 127 IARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIA 186 Query: 192 ELANKAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTNVGTHVYRRASEAGKRAQC 251 +L +AGLP GV NVV G + + DHP +KA+SF+GST + ++Y R S GKR Q Sbjct: 187 DLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQA 246 Query: 252 MMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKT-KEWIPEIVERSK 310 + GAKNH +V+PDA A++ L+GAA+G+AG+RCMA SV + VG E + + ER++ Sbjct: 247 LGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVAALAERAR 306 Query: 311 AMKVGPGSDRKADVGPLVSKAAKERVERLIGVGVEQGAKLLLDGRNCKVPGNESGNFVGP 370 +K+ G A++GP+V+ AAKER+E LIG GVE+GA L++DGR +VPG E G FVG Sbjct: 307 KLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKGFFVGG 366 Query: 371 TVFTGVKAGMDIYEQEIFGPAMCIVEVDTLDDAIAFINANPNGNGTSIFTSSGWAARKFQ 430 T+ V M +Y++EIFGP +C++ + A+ INA+ GNG +++T G AR+F Sbjct: 367 TLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGVAREFV 426 Query: 431 NEIDVGQVGINVP 443 +I+VG VG+NVP Sbjct: 427 RQIEVGMVGVNVP 439 Score = 94.0 bits (232), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 42/88 (47%), Positives = 59/88 (67%) Query: 601 AAAPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTNVGTHVYRRASEAGKRAQCMMGAK 660 A P GV NVV G + + DHP +KA+SF+GST + ++Y R S GKR Q + GAK Sbjct: 192 AGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQALGGAK 251 Query: 661 NHCIVLPDAPKDQAINNLLGAAFGAAGQ 688 NH +V+PDA A++ L+GAA+G+AG+ Sbjct: 252 NHMVVMPDADMQLAVDALMGAAYGSAGE 279 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 414 bits (1063), Expect = e-116, Method: Compositional matrix adjust. Identities = 219/444 (49%), Positives = 300/444 (67%), Gaps = 5/444 (1%) Query: 5 NIPNVPLYINGEHVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKEAFKTWRN 64 ++P++ +ING+ +S S +DV NPAT A+V A+ +EV+ AVA A A W Sbjct: 2 SLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAE 61 Query: 65 TSLAQRMRIMLKFQQLLRDNIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQ 124 T+ +R R++ KF++L+ N LA+ IT EHGK DA+GEV RGLE+VE A I L Sbjct: 62 TAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLL 121 Query: 125 LGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDP 184 + +N+ GG+D + + QP+GV AGIT FNFPVM+P +M PIA+A GN+FVLKPSE+DP Sbjct: 122 KTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 Query: 185 TSSLFLVELANKAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTNVGTHVYRRASE 244 + SL L EL +AGLP GV NVV G + + +HP+++AVSF+GST + ++YR+A+E Sbjct: 182 SPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATE 241 Query: 245 -AGK---RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKE 300 AG RAQ + GAKNH +V+PDA DQA++ L+GAA+G+AG+RCMA SV V VG + Sbjct: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 Query: 301 WIPE-IVERSKAMKVGPGSDRKADVGPLVSKAAKERVERLIGVGVEQGAKLLLDGRNCKV 359 + E ++ R KA+KV G + A++GPLV+ A K +VE I GV+ GAKLL+DGR KV Sbjct: 302 KLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKV 361 Query: 360 PGNESGNFVGPTVFTGVKAGMDIYEQEIFGPAMCIVEVDTLDDAIAFINANPNGNGTSIF 419 PG+E G F+G T+F V M IY++EIFGP +C+V V A+ INA+ GNG S+F Sbjct: 362 PGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLF 421 Query: 420 TSSGWAARKFQNEIDVGQVGINVP 443 T+ G A +F I VG VGINVP Sbjct: 422 TADGNTAHEFSRRIQVGMVGINVP 445 Score = 98.6 bits (244), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 59/161 (36%), Positives = 89/161 (55%), Gaps = 9/161 (5%) Query: 569 VDLMLKDLGLAVENALATGSSGPGPSRTRWLP-----AAAPPGVLNVVHGGPDVANMICD 623 V L + + +A N+ S PS + L A P GV NVV G + + + Sbjct: 157 VPLWMAPIAIATGNSFVLKPSERDPSPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLE 216 Query: 624 HPDIKAVSFIGSTNVGTHVYRRASE-AGK---RAQCMMGAKNHCIVLPDAPKDQAINNLL 679 HP+++AVSF+GST + ++YR+A+E AG RAQ + GAKNH +V+PDA DQA++ L+ Sbjct: 217 HPEVQAVSFVGSTPIAEYIYRKATERAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALI 276 Query: 680 GAAFGAAGQPPXLLGAAFGAARSASTCRSRCLWRISALPVR 720 GAA+G+AG+ + A A + R+ AL V+ Sbjct: 277 GAAYGSAGERCMAISVAVAVGSVADKLVEALIPRVKALKVK 317 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 268 bits (684), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 132/155 (85%), Positives = 140/155 (90%) Query: 445 LDAPVSGGTGGATAGTLTFMVGGTDDGFALARPYLEKMGKAIYHAGASGSGQTVKVCNNM 504 LDAPVSGGT GATAGTLTFMVGG+ + F A+PYL+KMGKAIYHAG SGSGQTVKVCNNM Sbjct: 118 LDAPVSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNM 177 Query: 505 LLGILMIGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYA 564 LLGILMIGTSEAIRLG+ANGMDPKVLSE+MSKSSGRNWTLEVYNPCPGVME APASKGYA Sbjct: 178 LLGILMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYA 237 Query: 565 GGFGVDLMLKDLGLAVENALATGSSGPGPSRTRWL 599 GGFGVDLMLKDLGLAVENALAT + P + R L Sbjct: 238 GGFGVDLMLKDLGLAVENALATNCAIPLGATARNL 272 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 213 bits (542), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 142/436 (32%), Positives = 214/436 (49%), Gaps = 6/436 (1%) Query: 8 NVPLYINGEHVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKEAFKTWRNTSL 67 +V L+INGE S S DV+NPAT+EV+ ++ A + ++DRA+ A + F+TWRN S Sbjct: 4 DVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSA 63 Query: 68 AQRMRIMLKFQQLLRDNIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGE 127 +R +IM + LLR+ +A L+T E GK L +A+ E + + ++ G Sbjct: 64 FERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGR 123 Query: 128 LAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSS 187 L G I +P+G A T +NFP+ A+A G + ++K E+ P S Sbjct: 124 LVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 188 LFLVELANKAGLPPGVLNVVHGGP-DVANMICDHPDIKAVSFIGSTNVGTHVYRRASEAG 246 L+ AG+P GV+N+V+G P +++ + HP IK +SF GST VG + A + Sbjct: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 247 KRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMA-NSVVVLVGKTKEWIPEI 305 KRA +G +V DA D A L A F AGQ C++ +V G ++ + Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKF 303 Query: 306 VERSKAMKVGPGSDRKADVGPLVSKAAKERVERLIGVGVEQGAKLLLDGRNCKVPGNESG 365 V ++ +KVG G + +GP+ +K +E LI V QGA+L G+ GN+ G Sbjct: 304 VTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRI---GNK-G 359 Query: 366 NFVGPTVFTGVKAGMDIYEQEIFGPAMCIVEVDTLDDAIAFINANPNGNGTSIFTSSGWA 425 F PTV T + +E FGP I +TLD+ I N P G F S Sbjct: 360 YFFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHT 419 Query: 426 ARKFQNEIDVGQVGIN 441 ++ G + IN Sbjct: 420 KHALAARVESGMLTIN 435 Score = 57.4 bits (137), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 35/89 (39%), Positives = 47/89 (52%), Gaps = 1/89 (1%) Query: 601 AAAPPGVLNVVHGGP-DVANMICDHPDIKAVSFIGSTNVGTHVYRRASEAGKRAQCMMGA 659 A P GV+N+V+G P +++ + HP IK +SF GST VG + A + KRA +G Sbjct: 193 AGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATMELGG 252 Query: 660 KNHCIVLPDAPKDQAINNLLGAAFGAAGQ 688 +V DA D A L A F AGQ Sbjct: 253 HAPAMVFDDADVDVAAKMLATAKFRNAGQ 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,263,967 Number of extensions: 96213 Number of successful extensions: 381 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 27 Length of query: 744 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 652 Effective length of database: 1,184,250 Effective search space: 772131000 Effective search space used: 772131000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)