BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0004.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 440 e-125 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 128 5e-31 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 124 9e-30 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 112 4e-26 gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 91 9e-20 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 440 bits (1131), Expect = e-125, Method: Compositional matrix adjust. Identities = 214/258 (82%), Positives = 232/258 (89%) Query: 5 TSYANLRLEKDGHTAIVTLANPPANTWTREGLVDLKRLVTDLNEDRDIYSLVVTGEGEKF 64 T+Y NL+LEK GHTA++TL+NPPANTWTR L DL RLV +LN DRDIY+LVVTGEGEKF Sbjct: 2 TTYTNLKLEKTGHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKF 61 Query: 65 FSAGADLKLFADGDKALAREMARRFGEAFETLSQFRGVSIAAINGYAMGGGLECALACDI 124 FSAGADLKLFADGDKA+AREMARRFGEAFETLS FRGVSIAAINGYAMGGGLECALACDI Sbjct: 62 FSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDI 121 Query: 125 RIAETQAQMALPEAAVGLLPCAGGTQLLPLLVGEGWAKRMILCGERVTAETALRIGLVEE 184 RIAE QAQMALPEAAVGLLPCAGGTQ LP LVGEGWAKRMILCGERV AETALRIGLVEE Sbjct: 122 RIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEE 181 Query: 185 VVEKGQARQRALALAKLVDKQSPSSVAASKKLIQGARHQPPMANLPVERELFLDLFSTED 244 VV +GQAR+RALALA+ +KQSPSSVAASK+LIQGARH P L ERELF+DLF T+D Sbjct: 182 VVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQD 241 Query: 245 QKEGVNAFLQKRSAEWKN 262 Q+EGV AFL+KRS +WKN Sbjct: 242 QREGVQAFLEKRSPQWKN 259 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 128 bits (321), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 90/262 (34%), Positives = 145/262 (55%), Gaps = 5/262 (1%) Query: 1 MTGSTSYANLRLEKDGHTAIVTLANPPA--NTWTREGLVDLKRLVTDLNEDRDIYSLVVT 58 M + +Y +L + G + N PA N + L ++ + ED + +V++ Sbjct: 1 MQDTVAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVIS 60 Query: 59 GEGEKFFSAGADLKLFADGDKALAREMARRFGEAFETLSQFRGVSIAAINGYAMGGGLEC 118 G EK F+AGADL D A+A ++ R + + +++F +AA+NGYA+G G E Sbjct: 61 G-NEKAFAAGADLNEMIHKD-AIATQLDVR-AQYWRAIARFPKPILAAVNGYALGAGCEL 117 Query: 119 ALACDIRIAETQAQMALPEAAVGLLPCAGGTQLLPLLVGEGWAKRMILCGERVTAETALR 178 + DI IA A++ PE VG LP AGGTQ L VG+ A +M+L GE ++A+ AL+ Sbjct: 118 LMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQ 177 Query: 179 IGLVEEVVEKGQARQRALALAKLVDKQSPSSVAASKKLIQGARHQPPMANLPVERELFLD 238 GLV EVV+ +R LALA + ++SP +V +K+ + + A L ER+ F Sbjct: 178 AGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSL 237 Query: 239 LFSTEDQKEGVNAFLQKRSAEW 260 + ++ D++EG+ AF +KR+A + Sbjct: 238 MAASADRQEGIAAFQEKRAAVF 259 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 124 bits (310), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 90/261 (34%), Positives = 133/261 (50%), Gaps = 7/261 (2%) Query: 6 SYANLRLEKDGHTAIVTLANPPANTWTREGLV-DLKRLVTDLNEDRDIYSLVVTGEGEKF 64 SY N+++E ++ L P A +GL+ +L + + +I +++TG EK Sbjct: 2 SYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGS-EKA 60 Query: 65 FSAGADLKLFADGD--KALAREMARRFGEAFETLSQFRGVSIAAINGYAMGGGLECALAC 122 F+AGAD+ A D E R +ETL + R IAA+ GYA+GGG E A+ C Sbjct: 61 FAAGADISAMAGYDYMDVFKGEFITR---NWETLRRIRKPVIAAVAGYALGGGCELAMMC 117 Query: 123 DIRIAETQAQMALPEAAVGLLPCAGGTQLLPLLVGEGWAKRMILCGERVTAETALRIGLV 182 D IA A+ PE +G++P AGGTQ LP V + A + L G + AE A R GLV Sbjct: 118 DFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLV 177 Query: 183 EEVVEKGQARQRALALAKLVDKQSPSSVAASKKLIQGARHQPPMANLPVERELFLDLFST 242 +V + + A+A A ++ + K + A + ER LF F+T Sbjct: 178 SRIVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFAT 237 Query: 243 EDQKEGVNAFLQKRSAEWKNA 263 EDQKEG+ AFL+KR +K++ Sbjct: 238 EDQKEGMKAFLEKRQPVFKHS 258 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 112 bits (279), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 83/260 (31%), Positives = 128/260 (49%), Gaps = 6/260 (2%) Query: 6 SYANLRLEKDGHTAIVTLANPPA-NTWTREGLVDLKRLVTDLNEDRDIYSLVVTGEGEKF 64 S ++ LE A++TL P N++T ++L+ + L D + L++TG G + Sbjct: 2 SSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAG-RG 60 Query: 65 FSAGADL--KLFADGDKA--LAREMARRFGEAFETLSQFRGVSIAAINGYAMGGGLECAL 120 F AG DL + GD L + + +G ++L I A+NG A G G L Sbjct: 61 FCAGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 Query: 121 ACDIRIAETQAQMALPEAAVGLLPCAGGTQLLPLLVGEGWAKRMILCGERVTAETALRIG 180 ACDI +A A +GL+P GGT LP LVG A M L GE+++AE A R G Sbjct: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 Query: 181 LVEEVVEKGQARQRALALAKLVDKQSPSSVAASKKLIQGARHQPPMANLPVERELFLDLF 240 L+ + V+ Q + A A+A + +AA+K + + L +ER+ L Sbjct: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 Query: 241 STEDQKEGVNAFLQKRSAEW 260 + D +EGV AFL+KR+ ++ Sbjct: 241 RSRDYREGVTAFLEKRAPQF 260 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 90.9 bits (224), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 81/264 (30%), Positives = 123/264 (46%), Gaps = 14/264 (5%) Query: 5 TSYANLRLEKDGHTAIVTLANPP-ANTWTREGLVDLKRLVTDLNEDRDIYSLVVTGEGEK 63 +S A+L + G A +TL P N + + + L L+T +D L++ G K Sbjct: 2 SSAAHLDITPRG-LATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTG-K 59 Query: 64 FFSAGADL---KLFADGDKALAREMARRFGEAFETLSQFRGVSIAAINGYAMGGGLECAL 120 FSAGADL + AD + AR+ ETL+ F ++A + G MGGG+ Sbjct: 60 SFSAGADLAWMRRMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLVS 119 Query: 121 ACDIRIAETQAQMALPEAAVGLLPCAGGTQLLPLLVGEGWAKRMILCGERVTAETALRIG 180 CDI +A A AL E +GL P A ++ + G A+R + ER A A +G Sbjct: 120 CCDIVVASDAAFFALSEVKLGLAPAAISPYVIAKM-GVSQARRYFVSAERFPAARAAAMG 178 Query: 181 LVEEVVEKGQARQRALALAKLVDKQSPSSVAASKKLIQGARHQPPMANLPVERE----LF 236 LV EV + R L + P ++ A+K+L+Q A P+ P E + Sbjct: 179 LVHEVAPPDELDARTAQLIDTLLANGPQAMRAAKQLVQVAN---PLQVTPELSEYTAGVI 235 Query: 237 LDLFSTEDQKEGVNAFLQKRSAEW 260 DL ++ + +EG+ AFL K + W Sbjct: 236 ADLRASAEGREGLRAFLDKDAPAW 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 801,321 Number of extensions: 32773 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 9 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)