BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0003.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 573 e-165 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 251 8e-68 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 165 4e-42 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 133 2e-32 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 116 2e-27 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 573 bits (1477), Expect = e-165, Method: Compositional matrix adjust. Identities = 280/325 (86%), Positives = 304/325 (93%) Query: 1 MGLSRLDATVVFEELAHACPSTAAFLTIHNMVSWMIASWATPEVKAAWCGDLAAGKKIGS 60 +GLSRLDATVVFEELA AC STAA+LTIHNMVSWMIASW P +KA WC LAAG+KIGS Sbjct: 62 LGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIGS 121 Query: 61 YCLTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTGGDGAKGVSA 120 YCLTEPGSGSDAASLKTTA+L DGHY++NGSKAFISGAG+TDVLV+MARTGG+GA+GVSA Sbjct: 122 YCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVSA 181 Query: 121 IVVPANTPGISYGKKEVKMGWNSQPTRTISFDNVKVPANHLLGAEGEGFKFAMKGLDGGR 180 IVVP N PG+SYGKKE KMGWNSQPTRTISFDNVKVPA+HLLG EG+GF FAMKGLDGGR Sbjct: 182 IVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGR 241 Query: 181 INIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVRLAA 240 INIATCSVGAAQAALDAAHAYMKERKQFGRP+ADFQALQFKLADMQTELVAARQMVRLAA Sbjct: 242 INIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAA 301 Query: 241 VKLDSKSPNATTYCAMAKRFATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQIL 300 KLD+ SP ATTYCAMAKR ATDLGF++CNEALQ+HGGYGYIREYPLERYFRDVRVHQIL Sbjct: 302 TKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQIL 361 Query: 301 EGTNEIMRVIIARHLLNNESNEDIR 325 EGTNEIMRVII+R LL+ ++ +DIR Sbjct: 362 EGTNEIMRVIISRKLLDRDNLDDIR 386 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 251 bits (640), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 132/322 (40%), Positives = 200/322 (62%), Gaps = 3/322 (0%) Query: 2 GLSRLDATVVFEELAHACPSTAAFLTIHNMVSWMIAS-WATPEVKAAWCGDLAAGKKIGS 60 G+ + + EE+A +T+ +++ N ++ I + +P K W LA G+ +G Sbjct: 61 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGC 120 Query: 61 YCLTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTG-GDGAKGVS 119 +CLTEP +GSDAA+++T A+ +I+NG+K FI+ V ++ A T G KG+S Sbjct: 121 FCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS 180 Query: 120 AIVVPANTPGISYGKKEVKMGWNSQPTRTISFDNVKVPANHLLGAEGEGFKFAMKGLDGG 179 +VP +TPG G+ E KMG ++ T I DN +VPA LLG EGEG++ A+ L+ G Sbjct: 181 CFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAG 240 Query: 180 RINIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVRLA 239 RI IA SVG A+AA +AA +Y ++R+ FG P+ + QA+ F+LADM T L AAR MV A Sbjct: 241 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRA 300 Query: 240 AVKLDSKSPNATTYCAMAKRFATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQI 299 A D P +MAK FA++ KI ++A+QIHGG GY ++P+ER +RDVR+ QI Sbjct: 301 AQLKDQGRP-CLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQI 359 Query: 300 LEGTNEIMRVIIARHLLNNESN 321 EG N+I R++I R + +++++ Sbjct: 360 YEGANDIQRLVIGRAIASDQAH 381 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 165 bits (418), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 107/325 (32%), Positives = 168/325 (51%), Gaps = 9/325 (2%) Query: 2 GLSRLDATVVFEELAHACPSTAAFLTIH-NMVSWMIASWATPEVKAAWCGDLAAGKKIGS 60 GL L + EE++ A S H N+ I E K + L +G IG+ Sbjct: 69 GLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGA 128 Query: 61 YCLTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTGGD-GAKGVS 119 ++EP +GSD S+K A Y++NGSK +I+ DVLV+ A+T + GA+G++ Sbjct: 129 LAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGMT 188 Query: 120 AIVVPANTPGISYGKKEVKMGWNSQPTRTISFDNVKVPANHLLGAEGEGFKFAMKGLDGG 179 A +V G S +K K+G T + F + +VP ++LG G G M GLD Sbjct: 189 AFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFE 248 Query: 180 RINIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAARQMVRLA 239 R ++ +G QA +D Y+ +RKQFG+ + +FQ +Q KLADM + ++A + V Sbjct: 249 RSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAV 308 Query: 240 AVKLDSKSPNATTYCAMAKRFATDLGFK------ICNEALQIHGGYGYIREYPLERYFRD 293 D ++ +A A+ K A + + + EA+Q GG GYI EYP+ R +RD Sbjct: 309 GQACD-RADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRD 367 Query: 294 VRVHQILEGTNEIMRVIIARHLLNN 318 ++++I GT+EI R++I R L + Sbjct: 368 AKLYEIGAGTSEIRRMLIGRELFAD 392 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 103/324 (31%), Positives = 170/324 (52%), Gaps = 14/324 (4%) Query: 2 GLSRLDATVVFEELAHAC-PSTAAFLTIHNMVSWMIASWATPEVKAAWCGDLAAGK-KIG 59 GL +A+V+ EE+ S A ++NM + + +PE K + +A+G+ ++ Sbjct: 64 GLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLL--RHGSPEQKERYLPRIASGELRLQ 121 Query: 60 SYCLTEPGSGSDAASLKTTAKLVDGHYIINGSKAFISGAGATDVLVLMARTG-----GDG 114 S +TEP +G+D +KT A Y+ING K +IS +D+++L+ART Sbjct: 122 SMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRK 181 Query: 115 AKGVSAIVVPANTPGISYGKKEVKMGWN--SQPTRTISFDNVKVPANHLLGAEGEGFKFA 172 ++G+S +V I +G EV+ N + T + FDN+++PA +L+G EG GF++ Sbjct: 182 SEGMSIFLVDLKE-AIGHGM-EVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYI 239 Query: 173 MKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRPLADFQALQFKLADMQTELVAA 232 + GL+ R IA +G +D A Y +ER F RP+ Q +QF LA+ E+ AA Sbjct: 240 LDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAA 299 Query: 233 RQMVRLAAVKLDSKSPNATTYCAMAKRFATDLGFKICNEALQIHGGYGYIREYPLERYFR 292 ++R A L + MAK A ++ N LQ HGG+G+ EY +ER FR Sbjct: 300 -NLMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFR 358 Query: 293 DVRVHQILEGTNEIMRVIIARHLL 316 + R++Q+ + ++ +A H+L Sbjct: 359 ETRLYQVAPISTNLIFSYVAEHVL 382 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 94/317 (29%), Positives = 153/317 (48%), Gaps = 37/317 (11%) Query: 36 IASWATPEVKAAWCGDLAAGKKIGSYCLTEPGSGSDAASLKTTA-KLVDGHYIINGSKAF 94 +A+ + ++A + +A+G+ + + CLTE +GSD L+T A DG Y ++G K F Sbjct: 139 LAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIF 198 Query: 95 ISGAGATD-----VLVLMAR--TGGDGAKGVSAIVVP-----ANTPGISYGKKEVKMGWN 142 ISG G D V +++AR G+KG+S + P + G+ E KMG + Sbjct: 199 ISG-GEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIH 257 Query: 143 SQPTRTISFDNVKVPANHLLGAEGEGFKFAMKGLDGGRINIATCSVGAAQAALDAAHAYM 202 T ++ F+ + L+G G G ++ R+++ +G A+AA A AY Sbjct: 258 GSATCSLHFEQAQA---WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYA 314 Query: 203 KERKQFGRP-------LADFQALQFKLADMQTELVAARQMVRLAAVKLD--SKSPNATTY 253 +ER+Q P + A+Q L + + + AR + A + LD P A Sbjct: 315 RERRQSRAPGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALR 374 Query: 254 CAMAKR----------FATDLGFKICNEALQIHGGYGYIREYPLERYFRDVRVHQILEGT 303 +R F TD GF+ + ALQ+ GG+GY+ E +E++ RD R+ I EGT Sbjct: 375 SRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGT 434 Query: 304 NEIMRV-IIARHLLNNE 319 NEI + + R +L +E Sbjct: 435 NEIQAIDFLMRKVLGDE 451 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,001,700 Number of extensions: 41358 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 12 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)