BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0077.0003.p (217 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 86 2e-18 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 83 1e-17 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 81 9e-17 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 72 3e-14 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 72 5e-14 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 85.9 bits (211), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 61/203 (30%), Positives = 108/203 (53%), Gaps = 9/203 (4%) Query: 13 MTGAGDRAFCAGQDLEEAHGFD--EQRADEWMT-EWENYYRTLRSLKKPLVMALNGTAAG 69 +TG+ ++AF AG D+ G+D + E++T WE TLR ++KP++ A+ G A G Sbjct: 54 ITGS-EKAFAAGADISAMAGYDYMDVFKGEFITRNWE----TLRRIRKPVIAAVAGYALG 108 Query: 70 SAFQVTLLGDIRVGHAGSRMGQPEINSGI-PSVTGPWIMTQMLGLSRTIELTLTGRMMEA 128 ++ ++ D + ++ GQPEI GI P G + + + ++ ++L LTGRMM+A Sbjct: 109 GGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDA 168 Query: 129 EEAHRLGLIHHLVPAAEVMSKSLAIARELAAKPPVAMRLDKQRFYEITEPSFVDAIAAGR 188 EEA R GL+ +V A +++ +++A A +A P + K E + + + R Sbjct: 169 EEAERAGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYER 228 Query: 189 RIQGEAYATGEPARMMEEFFKKR 211 + +AT + M+ F +KR Sbjct: 229 ALFYSCFATEDQKEGMKAFLEKR 251 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 83.2 bits (204), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 49/165 (29%), Positives = 89/165 (53%), Gaps = 9/165 (5%) Query: 17 GDRAFCAGQDLEEAHGFDEQRADEWMTEWE---NYYRTLRSLKKPLVMALNGTAAGSAFQ 73 ++AF AG DL E D T+ + Y+R + KP++ A+NG A G+ + Sbjct: 62 NEKAFAAGADLNEM-----IHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCE 116 Query: 74 VTLLGDIRVGHAGSRMGQPEINSG-IPSVTGPWIMTQMLGLSRTIELTLTGRMMEAEEAH 132 + + DI + G+++GQPEIN G +P G + + +G +++ L+G + A++A Sbjct: 117 LLMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQAL 176 Query: 133 RLGLIHHLVPAAEVMSKSLAIARELAAKPPVAMRLDKQRFYEITE 177 + GL+ +V + ++LA+A +A K P+A+RL K+ + E Sbjct: 177 QAGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFE 221 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 80.9 bits (198), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 54/208 (25%), Positives = 92/208 (44%), Gaps = 1/208 (0%) Query: 5 SYGKIHQXMTGAGDRAFCAGQDLEEAHGFDEQRADEWMTE-WENYYRTLRSLKKPLVMAL 63 S + + A +AFCAG DL + +Q E + + ++LR L P++ + Sbjct: 48 SDSTVRCVILAANGKAFCAGHDLRQMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKV 107 Query: 64 NGTAAGSAFQVTLLGDIRVGHAGSRMGQPEINSGIPSVTGPWIMTQMLGLSRTIELTLTG 123 G A + Q+ D+ + +R IN G+ T +T+ L + R E+ +TG Sbjct: 108 QGLATAAGCQLVATCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTG 167 Query: 124 RMMEAEEAHRLGLIHHLVPAAEVMSKSLAIARELAAKPPVAMRLDKQRFYEITEPSFVDA 183 + ++A A GLI+ VP E+ S + A+ARE+ +KP +++ K Y + DA Sbjct: 168 KFIDAATAAAWGLINQAVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDA 227 Query: 184 IAAGRRIQGEAYATGEPARMMEEFFKKR 211 A + E + F KR Sbjct: 228 YAHAAEVMACNIMDPEAQEGITAFLDKR 255 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 72.4 bits (176), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 50/166 (30%), Positives = 81/166 (48%), Gaps = 6/166 (3%) Query: 13 MTGAGDRAFCAGQDLEEAHGFDEQRADEWMTEWENYY----RTLRSLKKPLVMALNGTAA 68 +TGAG R FCAGQDL + + + YY ++LR+L P++ A+NG AA Sbjct: 54 LTGAG-RGFCAGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAA 112 Query: 69 GSAFQVTLLGDIRVGHAGSRMGQPEINSG-IPSVTGPWIMTQMLGLSRTIELTLTGRMME 127 G+ + L DI + + + G IP G + + +++G +R + + L G + Sbjct: 113 GAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKIS 172 Query: 128 AEEAHRLGLIHHLVPAAEVMSKSLAIARELAAKPPVAMRLDKQRFY 173 AE+A R GLI V ++M ++ A+A A P + K Y Sbjct: 173 AEQAERWGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLY 218 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 71.6 bits (174), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 45/159 (28%), Positives = 78/159 (49%), Gaps = 1/159 (0%) Query: 13 MTGAGDRAFCAGQDLEEAHGFDEQRADEWMTEWENYYRTLRSLKKPLVMALNGTAAGSAF 72 +TG G++ F AG DL+ D+ A E + + TL + + + A+NG A G Sbjct: 54 VTGEGEKFFSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGL 113 Query: 73 QVTLLGDIRVGHAGSRMGQPEINSG-IPSVTGPWIMTQMLGLSRTIELTLTGRMMEAEEA 131 + L DIR+ ++M PE G +P G + ++G + L G + AE A Sbjct: 114 ECALACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETA 173 Query: 132 HRLGLIHHLVPAAEVMSKSLAIARELAAKPPVAMRLDKQ 170 R+GL+ +VP + ++LA+A++ + P ++ KQ Sbjct: 174 LRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASKQ 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 712,483 Number of extensions: 29544 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 7 Length of query: 217 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 136 Effective length of database: 1,236,335 Effective search space: 168141560 Effective search space used: 168141560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)