BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0057.p (342 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 222 3e-59 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 198 6e-52 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 167 9e-43 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 151 6e-38 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 76 5e-15 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 222 bits (566), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 113/336 (33%), Positives = 186/336 (55%), Gaps = 5/336 (1%) Query: 3 GKSGRDAALLAIDEINARPLYIAGEKIVLKLLDQDDKADVNIAILTARYFIASKVVAVVG 62 GKS ++ LA+ E N R L I G+ L+L+ QDD+AD A AR + +V+AVVG Sbjct: 56 GKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRADPATAEFVARSLVQQQVIAVVG 115 Query: 63 HWNSAASIATAKIYSDAGIAEISPSSTSTEYTQQGLPTTFRMVGHDGIRTNYIGEHVLRT 122 HW S S+A A +Y AG+ +++PS+ S T P FR + ++ + + Sbjct: 116 HWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRIFRTIPNNESLGRLAAGYAVDK 175 Query: 123 LRAERIMVIDDATLFGKAIADQFINYIQANGGLISGRMSISNKTSNYALALESVRQARPD 182 L I+ IDD T FG+ +A+QF G + GR S+S+KTS++ AL R+ +PD Sbjct: 176 LETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYSVSDKTSDFNAALLEARKLQPD 235 Query: 183 LIFHAGRLYSGDLNHSESLLGGLRRLGFPKNMVLAEVTIDPESMREAALGDINIFSISPG 242 LIF G + + L +RRL ++ + T+ + A + PG Sbjct: 236 LIFFGGLDWQAGV-----LAKSIRRLKLHARLMASPGTLGLPFLMRAGPDANGALVLEPG 290 Query: 243 IPFEKLPRWKSFQKNFTARFSSRITPYTLSAYDAVYVLAEAMKQAGSLDPPRISAILHNI 302 P +K+P WKSF++ ++ F S + Y + AY+A + + ++QAGS DP RI+ +H + Sbjct: 291 PPQDKMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIRQAGSADPERIAEAMHRL 350 Query: 303 KYVGLTGPISFDAKGDVTSPSYTLYQIKQGAWVPIS 338 ++ G++GP++F+ +GD+ PS+T+Y++K W P++ Sbjct: 351 RFEGVSGPVAFNEEGDLLQPSFTMYEVKDQRWQPVA 386 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 198 bits (503), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 115/340 (33%), Positives = 182/340 (53%), Gaps = 8/340 (2%) Query: 3 GKSGRDAALLAIDEINARPLYIAGEKIVLKLLDQDDKADVNIAILTARYFIASKVVAVVG 62 GK + A +A+DE+NA+ I G+K+ +L+ +DD +D A A + KV AV+G Sbjct: 44 GKDNENGARMAVDELNAKGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIG 103 Query: 63 HWNSAASIATAKIYSDAGIAEISPSSTSTEYTQQGLPTTFRMVGHDGIRTNYIGEHVLRT 122 H NS +I +KIYSDAGI ++SPS+T+ +YTQQG T FR+V +D +G++ ++ Sbjct: 104 HLNSGTTIPASKIYSDAGIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQK 163 Query: 123 LRAERIMVIDDATLFGKAIADQFINYIQANGGLISGRMSISNKTSNYALALESVRQARPD 182 L ++I VIDD T +G+ +AD+F +A GG I G ++K +++ L S++ +PD Sbjct: 164 LGTKQIAVIDDRTAYGQGVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPD 223 Query: 183 LIFHAGRLYSGDLNHSESLLGGLRRLGFPKNMVLAE--VTIDPESMREAALGDINIFSIS 240 ++F G G +L +++LG + + T + LGD + Sbjct: 224 VVFFGGMDAVGG-----PMLRQMKQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAE 278 Query: 241 PGIPFEKLPR-WKSFQKNFTARFSSRITPYTLSAYDAVYVLAEAMKQAGSLDPPRISAIL 299 G E + F+ + +F + AYDA +A+AM +AGS DP + L Sbjct: 279 AGGVDEAGKKGMDDFRAAYKKKFGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPEL 338 Query: 300 HNIKYVGLTGPISFDAKGDVTSPSYTLYQIKQGAWVPISV 339 I + G+TG I+FDAKGD+ S TLY K G ++V Sbjct: 339 AKISHKGVTGVIAFDAKGDIKDGSLTLYTYKGGQRTLLAV 378 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 167 bits (424), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 96/338 (28%), Positives = 176/338 (52%), Gaps = 9/338 (2%) Query: 3 GKSGRDAALLAIDEINARPLYIAGEKIVLKLLDQDDKADVNIAILTARYFIASKVVAVVG 62 GK + ++AI+ +NA+P+ + G+KI ++ +DD+AD + A+ V A+VG Sbjct: 46 GKDNQGGLMMAIERLNAQPITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVG 105 Query: 63 HWNSAASIATAKIYSDAGIAEISPSSTSTEYTQQGLPTTFRMVGHDGIRTNYIGEHVLRT 122 +NS +I +++Y+DAGI ++ +++ + TQQG T FR+ D + + + Sbjct: 106 PYNSGVTIPASRVYNDAGIV-VATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKE 164 Query: 123 LRAERIMVIDDATLFGKAIADQFINYIQANGGLISGRMSISNKTSNYALALESVRQARPD 182 L+ +R+ VIDD T +G+ +A +FI +ANG + ++K +++ L S++ +PD Sbjct: 165 LKFKRVAVIDDRTAYGQGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPD 224 Query: 183 LIFHAGRLYSGDLNHSESLLGGLRRLGFPKNMVLAEVTIDPESMREA--ALGDINIFSIS 240 +F G G + +++LG ++ + PE R A+G+ +++ Sbjct: 225 AVFLGGYAPQGGPIKRQ-----MKQLGVDVPLMGGDGICSPEMGRLGGDAIGE-SVYCTQ 278 Query: 241 PGIPFEKLPRWKSFQKNFTARFSSRITPYTLSAYDAVYVLAEAMKQAGSLDPPRISAILH 300 G +K K F + +++ Y +S YD + ++A+AMKQA S+DP + L Sbjct: 279 GGTMLDKAKEGKVFSDEYQKKYNRPAETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALA 338 Query: 301 NIKYVGLTGPISFDAKGDVTSPSYTLYQIKQGAWVPIS 338 I Y G+ G FDA D+ T+Y+ K G VP++ Sbjct: 339 KISYKGVAGQYDFDANHDLKQSPVTVYRFKDGLPVPLT 376 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 151 bits (382), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 103/341 (30%), Positives = 172/341 (50%), Gaps = 17/341 (4%) Query: 4 KSGRDAALLAIDEINARPLYIAGEK---IVLKLLDQDDKADVNIAILTARYFIASKVVAV 60 +S A LAIDE NA +A ++ + +LL QDD + N A+ ARY++ KV V Sbjct: 45 QSALQGAQLAIDEANA----LAAKRRSPLRFELLPQDDHGNENFAVNIARYYVKEKVAGV 100 Query: 61 VGHWNSAASIATAKIYSDAGIAEISPSSTSTEYTQQGLPTTFRMVGHDGIRTNYIGEHVL 120 +G W+S A++ATA IY A I +I ++TS+++T G FR+VG + E Sbjct: 101 IGPWSSDAALATAAIYESAQIPQIGFTATSSQWTGMGNRMPFRVVGGTRELAATLAEVAT 160 Query: 121 RTLRAERIMVIDDATLFGKAIADQFINYIQANGGLIS-GRMSISNKTSNYALALESVRQA 179 R+L+ RI VI + +A+AD + + + I+ R + +++++ AL++ Sbjct: 161 RSLQGRRIFVIQSDAAYSRALADTLVENLGGSAQQIALARYQVGRRSTDFHAALKAAEAH 220 Query: 180 RPDLIFHAGRLYSGDLNHSESLLGGLRRLGFPKNMVLAEVTIDPESMREAALGDINIFSI 239 + D+I G LY + + + G R ++L T ++ A ++ + + Sbjct: 221 QADVIVFVG-LYP----QARAFIDGANRTRTTARLLL---TGGASNLSLPAPLELRLHVL 272 Query: 240 SPGIPFEKLPRWKSFQKNFTARFSSRITPYTLSAYDAVYVLAEAMKQAGSLDPPRISAIL 299 I P+WK + + RF + T Y+ AYDA VL A+ QAGS + +I+ L Sbjct: 273 EYEIARPHCPQWKVVTQTYQRRFGAAPTTYSFYAYDAASVLTAAIVQAGSDNGDKIATAL 332 Query: 300 HNIKYVGLTGPISFDAKGDVTSPSYTLYQIKQGAWVPISVL 340 H +++ GL G +SFDA G P YTL + ++ W + V Sbjct: 333 HRMRHAGLHGTLSFDATGANAHPRYTLLRYQE-RWQAVEVF 372 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 75.9 bits (185), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 77/332 (23%), Positives = 149/332 (44%), Gaps = 30/332 (9%) Query: 7 RDAALLAIDEINARPLYIAGEKIVLKLLDQDDKADVNIAILTARYFIASKVVAVVGHWNS 66 + AL AI++INA G K ++D D + A+ A ++ + V+GH S Sbjct: 49 KAGALTAIEQINAAG-GANGNKFEAVMMD--DACEPKQAVAVANKIVSQGIKYVIGHVCS 105 Query: 67 AASIATAKIYSDAGIAEISPSSTSTEYTQ-QGLPTTFRMVGHDGIRTNYIGEHVLRTLRA 125 ++I + IY + G+ I+PS+T+ + T+ + FR +G D + +V+ L+ Sbjct: 106 GSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKP 165 Query: 126 ERIMVIDDATLFGKAIADQFINYIQANGGLISGRMSISNKTSNYALALESVRQARPDLIF 185 +++ V+ D +G+ +A + A + I+ S+Y+ + ++ D ++ Sbjct: 166 KKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVY 225 Query: 186 HAGRLYSGDLNHSESLLGGLRRLGFPKNMVLAEVTIDPESMREAALGDINIFSI----SP 241 G H E +G + R + + V + PE +G+ +I +I S Sbjct: 226 FGGY-------HPE--MGLIMRQA--REQGIKAVFMGPEG-----VGNKDITAIAGPASE 269 Query: 242 GI------PFEKLPRWKSFQKNFTARFSSRITPYTLSAYDAVYVLAEAMKQAGSLDPPRI 295 G+ F P + K F A P+ + AY V ++A+A+ A S DP ++ Sbjct: 270 GMLVTLPADFAADPANAALVKAFAAAKRDPNGPFQMPAYSGVKIIADAIAGAKSTDPEKV 329 Query: 296 SAILHNIKYVGLTGPISFDAKGDVTSPSYTLY 327 +A +H + G + +D KGD+ S + ++ Sbjct: 330 AAYIHANSFKTPIGNVEYDKKGDLKSFKFVVF 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 989,241 Number of extensions: 37576 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 5 Length of query: 342 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 256 Effective length of database: 1,212,660 Effective search space: 310440960 Effective search space used: 310440960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)