BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0056.p (158 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 250 4e-68 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 167 3e-43 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 103 8e-24 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 96 1e-21 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 96 2e-21 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 250 bits (639), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 112/150 (74%), Positives = 133/150 (88%) Query: 9 KNAVAKMEEHIADALGKGGKVITGGKRHALGQTFFEPTVVCDANSRMLVAKEETFGPLAP 68 + AV K+E+H+ADA+ KG +V+ GGKRHALG +FFEPTV+ D M VA+EETFGP+AP Sbjct: 335 EQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTPAMQVAREETFGPMAP 394 Query: 69 LFRFKSDDEVIGLANDTEFGLASYFYTRDIGRVWRIAEGLEYGMVGINTGVISNEMAPFG 128 LFRFK+D+EV+ LANDTEFGLASYFY+RDIGR+WR+AEGLE GMVGINTG+ISNE+APFG Sbjct: 395 LFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFG 454 Query: 129 GVKQSGIGREGSKYGLDDYHVTKYLCMGGI 158 GVKQSG+GREGS YG+DDY V KYLCMGGI Sbjct: 455 GVKQSGLGREGSHYGIDDYLVVKYLCMGGI 484 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 167 bits (424), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 79/153 (51%), Positives = 109/153 (71%), Gaps = 4/153 (2%) Query: 10 NAVAKMEEHIADALGKGGKVITGGKR---HALGQ-TFFEPTVVCDANSRMLVAKEETFGP 65 A+AK+++H+ DA+ +G +VITGGKR G ++ PTV+ D M + EETFGP Sbjct: 341 RAIAKIDQHVRDAIARGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGP 400 Query: 66 LAPLFRFKSDDEVIGLANDTEFGLASYFYTRDIGRVWRIAEGLEYGMVGINTGVISNEMA 125 +AP+ RF ++DEVI AN T FGLA+YFY+ D+ R+ R+ + LE G+VG+N G ++ E A Sbjct: 401 VAPITRFATEDEVIAAANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAA 460 Query: 126 PFGGVKQSGIGREGSKYGLDDYHVTKYLCMGGI 158 PFGGVK+SG GREGS +GLDDY TKY+C G + Sbjct: 461 PFGGVKESGYGREGSVHGLDDYLHTKYVCQGQL 493 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 103 bits (256), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 50/142 (35%), Positives = 83/142 (58%) Query: 14 KMEEHIADALGKGGKVITGGKRHALGQTFFEPTVVCDANSRMLVAKEETFGPLAPLFRFK 73 ++ +I L +G ++ GG F +PT+ DAN+ M +A+EE FGP+ + F Sbjct: 339 RVNRYIQQGLDEGATLLAGGVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFD 398 Query: 74 SDDEVIGLANDTEFGLASYFYTRDIGRVWRIAEGLEYGMVGINTGVISNEMAPFGGVKQS 133 +++E I LAND+++GLA+ +T ++ R R+A G++ G VG+N + P+GG+K S Sbjct: 399 TEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDS 458 Query: 134 GIGREGSKYGLDDYHVTKYLCM 155 G+GREG G Y K + + Sbjct: 459 GMGREGGLAGALAYTEEKTVTV 480 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 95.9 bits (237), Expect = 1e-21, Method: Composition-based stats. Identities = 48/130 (36%), Positives = 75/130 (57%), Gaps = 4/130 (3%) Query: 19 IADALGKGGKVITGGKRHALGQTFFEPTVVCDANSRMLVAKEETFGPLAPLFRFKSDDEV 78 I DAL KG ++ GG+ T T++ ++ ML+ +EE+FGP+ P+ R D+ Sbjct: 340 IDDALAKGATLVCGGRA---SDTLMPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAA 396 Query: 79 IGLANDTEFGLASYFYTRDIGRVWRIAEGLEYGMVGINTGVISNE-MAPFGGVKQSGIGR 137 I AND E+GL+S ++RD+ R ++ +E G+ IN + +E PFGGVK SG G Sbjct: 397 IACANDNEYGLSSALFSRDVARALALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFGH 456 Query: 138 EGSKYGLDDY 147 G K G+ ++ Sbjct: 457 FGGKAGIAEF 466 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 95.5 bits (236), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 54/142 (38%), Positives = 75/142 (52%), Gaps = 4/142 (2%) Query: 14 KMEEHI----ADALGKGGKVITGGKRHALGQTFFEPTVVCDANSRMLVAKEETFGPLAPL 69 K EHI A L G + G R+ F PT++ A++ M VA+ E FGP+A Sbjct: 345 KQYEHIQRMVARGLEAGATLAAGHTRYGEEGYFVRPTLLAHASNAMEVARSEIFGPVAVA 404 Query: 70 FRFKSDDEVIGLANDTEFGLASYFYTRDIGRVWRIAEGLEYGMVGINTGVISNEMAPFGG 129 F+ ++E I +AND+EFGLA +TRD+ R R+A + G +N+ N +PFGG Sbjct: 405 IPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVAASVNAGTFWVNSYKTINVASPFGG 464 Query: 130 VKQSGIGREGSKYGLDDYHVTK 151 +SG GR L DY TK Sbjct: 465 FNRSGYGRSSGMEALYDYTQTK 486 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 545,736 Number of extensions: 23196 Number of successful extensions: 79 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 74 Number of HSP's successfully gapped: 14 Length of query: 158 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 80 Effective length of database: 1,250,540 Effective search space: 100043200 Effective search space used: 100043200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)