BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0075.0052.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 237 1e-63 gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 231 7e-62 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 179 4e-46 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 237 bits (605), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 167/476 (35%), Positives = 240/476 (50%), Gaps = 43/476 (9%) Query: 2 ENYDLVVVGGGPGGYVAAIRAAQLGLKTALVEKAQL-------GGICLNWGCIPTKALLR 54 +N+D+VV+GGGPGGY+AAIRAAQLG TA +++ + GG C N GCIP+KALL+ Sbjct: 3 KNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQ 62 Query: 55 SADILRLVKDA-GRFGVQVAAPQIDLAAMVKRSRDVAGQLQSGVTYLMKKNKIAVIAGHA 113 S++ G++V ++L M+ R V Q G+ YL KKNK++ G Sbjct: 63 SSEHYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVSFFHGRG 122 Query: 114 R-------------------------------ARARPLPGLTPDGESVWSYREALMPTRV 142 + AR LPG D + + S AL T V Sbjct: 123 SFVKGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSITEV 182 Query: 143 PTHLVVVGAGAIGIEFASFYHALGAKVSVVEMAERVLPVEDEDISRHMGDALRKQGMQLY 202 P L V+GAG IG+E S + LGA+V+V+E L DE I++ L KQG+ + Sbjct: 183 PKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQGLAVN 242 Query: 203 LGSRLTSSSKRRNGGVDEWTLQLDGRHKESLTADVVLTAAGIVGNIEELGLEATAVKID- 261 LG ++ + +N E+T H D ++ + G N L E +K+D Sbjct: 243 LGVKIGAIKAGKNSVSVEYTDAKGDAHTGEF--DKLIVSIGRTPNTIGLNAEGVGLKLDE 300 Query: 262 RSHVVTDAYGATGEPGVYAIGDLAGAPWLAHKASHEAVICVEKIAGMNPHALDATRIPAC 321 R + D T P V+A+GD+ P LAHKA E V E+IAG + H ++ IP Sbjct: 301 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGH-VNFNTIPWV 359 Query: 322 TYSHPQVASVGLTEAQAKANGHKVRIGTFPLAVNGKAIALGATGGFAKVVFDDASGELLG 381 Y+ P++A VG TE Q KA G + + GTFP NG+A ALG T G K + D + E+LG Sbjct: 360 IYTSPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILG 419 Query: 382 AHMVGEEVTEMIQGYAIARELESTEAELMETVFPHPTVSESMHEAVLAAYGRALHI 437 H+VG +E+I +A E ++ ++ HP++SE+ EA LA RAL+ Sbjct: 420 VHIVGPMASELISEAVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRALNF 475 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 231 bits (590), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 156/460 (33%), Positives = 231/460 (50%), Gaps = 34/460 (7%) Query: 5 DLVVVGGGPGGYVAAIRAAQLGLKTALVEK-AQLGGICLNWGCIPTKALLRSADILRLVK 63 D++V+GGGPGGY AA RAA LGL T +VE+ A LGG+CLN GCIP+KALL A ++ Sbjct: 128 DVMVLGGGPGGYSAAFRAADLGLNTVIVEREATLGGVCLNVGCIPSKALLHVAAVIDETA 187 Query: 64 DAGRFGVQVAAPQIDLAAMVKRSRDVAGQLQSGVTYLMKKNKIAVIAGHAR--------- 114 GV PQID+ + V G + G+ + K K+ V+ G + Sbjct: 188 AMASHGVTFGKPQIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQFAGPNHIEV 247 Query: 115 --------------------ARARPLPGLTPDGESVWSYREALMPTRVPTHLVVVGAGAI 154 + LP P + AL +VP ++V+G G I Sbjct: 248 TAADGSKKVVQFKHAIIAAGSSVVNLP-FVPQDPRIVDSTGALELRQVPKRMLVIGGGII 306 Query: 155 GIEFASFYHALGAKVSVVEMAERVLPVEDEDISRHMGDALRKQGMQLYLGSRLTSSSKRR 214 G+E A+ Y LGA++ VVEM + ++ D D+ + K+ + + ++ + Sbjct: 307 GLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKTKTVAVEALP 366 Query: 215 NG-GVDEWTLQLDGRHKESLTADVVLTAAGIVGNIEELGLEATAVKI-DRSHVVTDAYGA 272 G V + E D+VL A G N ++L + V + DR + D Sbjct: 367 EGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRGFIAVDKQMR 426 Query: 273 TGEPGVYAIGDLAGAPWLAHKASHEAVICVEKIAGMNPHALDATRIPACTYSHPQVASVG 332 T P ++AIGDL G P LAHKA HE + E IAG DA+ IP+ Y+ P+VA VG Sbjct: 427 TNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSF-FDASVIPSVAYTDPEVAWVG 485 Query: 333 LTEAQAKANGHKVRIGTFPLAVNGKAIALGATGGFAKVVFDDASGELLGAHMVGEEVTEM 392 +TE +AKA G K+ G FP A +G+A+A G + GF K++FD + ++G +VG +M Sbjct: 486 VTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGIVGTHAGDM 545 Query: 393 IQGYAIARELESTEAELMETVFPHPTVSESMHEAVLAAYG 432 I A+A E+ + ++ +T+ PHPT+ ES+ A A G Sbjct: 546 IGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAAEVAEG 585 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 179 bits (454), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 129/447 (28%), Positives = 209/447 (46%), Gaps = 27/447 (6%) Query: 3 NYDLVVVGGGPGGYVAAIRAAQLGLKTALVEKAQLGGICLNWGCIPTKALLRSADILRLV 62 +YDL +GGG GG AA A+Q G + L EK LGG C+N GCIP K + SA Sbjct: 5 DYDLFTIGGGSGGVRAARFASQAGARVGLAEKGDLGGTCVNLGCIPKKLMSYSAHYHEEF 64 Query: 63 KDAGRFGVQV-AAPQIDLAA-MVKRSRDVAGQLQSGVTYLMKKNKIAVIAGHARAR---- 116 DA +G + P D +A M + R++A L L+ K+++ G A+ Sbjct: 65 ADAAGYGWTLNGQPSFDWSALMANKDREIA-HLNDIYLRLLDNAKVSLHRGFAKVEDAHT 123 Query: 117 ------------------ARPLPGLTPDGESVWSYREALMPTRVPTHLVVVGAGAIGIEF 158 RP P E + + +P VV+G G I +E Sbjct: 124 VNVDGQRFTARHILVATGGRPDKPAIPGAELGITSDDFFHLKALPQRAVVLGGGYIAVEL 183 Query: 159 ASFYHALGAKVSVVEMAERVLPVEDEDISRHMGDALRKQGMQLYLGSRLTSSSKRRNGGV 218 AS + LG +V++V ER+L D D+ H+ D + K+G+++ + + + + G Sbjct: 184 ASILNGLGCEVTLVYRRERLLRNMDADLGIHLADEMAKKGIRIVFNASIEAIEAEQAGEA 243 Query: 219 DEWTLQ-LDGRHKESLTADVVLTAAGIVGNIEELGLEATAVKIDRSHVV-TDAYGATGEP 276 E +++ + + E+L+A VL A G N LGL+ VK+ + + + + P Sbjct: 244 SEASVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQEAGVKLKANGAIEVNQDFESSVP 303 Query: 277 GVYAIGDLAGAPWLAHKASHEAVICVEKIAGMNPHALDATRIPACTYSHPQVASVGLTEA 336 + A+GD+ L A EA+ V ++ G + IP +SHP V +VGL+E Sbjct: 304 SILAVGDVIDRVALTPVALAEAMAVVSRLFGKGERGMSYANIPTAVFSHPNVGTVGLSEE 363 Query: 337 QAKANGHKVRIGTFPLAVNGKAIALGATGGFAKVVFDDASGELLGAHMVGEEVTEMIQGY 396 +A+ ++RI ++ F K+V D + +LG HMVG + E+IQG+ Sbjct: 364 EARQQFGELRIFKTDFKPLKNTLSRNTERTFMKLVVDARTDRVLGVHMVGGDAGEVIQGF 423 Query: 397 AIARELESTEAELMETVFPHPTVSESM 423 A+A + +T+A+ T+ HPT +E Sbjct: 424 AVALQCGATKAQFDTTIGIHPTSAEEF 450 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,369,236 Number of extensions: 60366 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 4 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)